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Annals of Biomedical Engineering

, Volume 42, Issue 11, pp 2314–2321 | Cite as

Rapid Antimicrobial Susceptibility Testing with Electrokinetics Enhanced Biosensors for Diagnosis of Acute Bacterial Infections

  • Tingting Liu
  • Yi Lu
  • Vincent Gau
  • Joseph C. Liao
  • Pak Kin WongEmail author
Article

Abstract

Rapid pathogen detection and antimicrobial susceptibility testing (AST) are required in diagnosis of acute bacterial infections to determine the appropriate antibiotic treatment. Molecular approaches for AST are often based on the detection of known antibiotic resistance genes. Phenotypic culture analysis requires several days from sample collection to result reporting. Toward rapid diagnosis of bacterial infection in non-traditional healthcare settings, we have developed a rapid AST approach that combines phenotypic culture of bacterial pathogens in physiological samples and electrochemical sensing of bacterial 16S rRNA. The assay determines the susceptibility of pathogens by detecting bacterial growth under various antibiotic conditions. AC electrokinetic fluid motion and Joule heating induced temperature elevation are optimized to enhance the sensor signal and minimize the matrix effect, which improve the overall sensitivity of the assay. The electrokinetics enhanced biosensor directly detects the bacterial pathogens in blood culture without prior purification. Rapid determination of the antibiotic resistance profile of Escherichia coli clinical isolates is demonstrated.

Keywords

Electrokinetic enhancement Point-of-care Antimicrobial susceptibility testing Acute bacterial infections Electrochemical sensing 

Notes

Acknowledgments

This work was supported by NIH Health Director’s New Innovator Award (1DP2OD007161-01) and NIAID (1U01AI082457-01 and 2R44AI088756-03).

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Copyright information

© Biomedical Engineering Society 2014

Authors and Affiliations

  • Tingting Liu
    • 1
  • Yi Lu
    • 1
  • Vincent Gau
    • 2
  • Joseph C. Liao
    • 3
    • 4
  • Pak Kin Wong
    • 1
    Email author
  1. 1.Department of Aerospace and Mechanical EngineeringThe University of ArizonaTucsonUSA
  2. 2.GeneFluidics Inc.IrwindaleUSA
  3. 3.Department of UrologyStanford UniversityStanfordUSA
  4. 4.Veterans Affairs Palo Alto Health Care SystemPalo AltoUSA

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