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Annals of Biomedical Engineering

, Volume 42, Issue 4, pp 822–832 | Cite as

Quantification of a Thermal Damage Threshold for Astrocytes Using Infrared Laser Generated Heat Gradients

  • Rickard LiljemalmEmail author
  • Tobias NybergEmail author
Article

Abstract

The response of cells and tissues to elevated temperatures is highly important in several research areas, especially in the area of infrared neural stimulation. So far, only the heat response of neurons has been considered. In this study, primary rat astrocytes were exposed to infrared laser pulses of various pulse lengths and the resulting cell morphology changes and cell migration was studied using light microscopy. By using a finite element model of the experimental setup the temperature distribution was simulated and the temperatures and times to induce morphological changes and migration were extracted. These threshold temperatures were used in the commonly used first-order reaction model according to Arrhenius to extract the kinetic parameters, i.e., the activation energy, E a, and the frequency factor, A c, for the system. A damage signal ratio threshold was defined and calculated to be 6% for the astrocytes to change morphology and start migrating.

Keywords

Arrhenius Heating Cell damage Modeling Astrocytes 

Notes

Acknowledgments

Funding was received from the Product Innovation Engineering Program (PIEp) through the Innovation Driven Research Education (IDRE). We are grateful to Professor Hans Hebert at the School for Technology and Health at the Royal Institute of technology for providing the digital microscope.

Supplementary material

AVI (3502 KB)

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Copyright information

© Biomedical Engineering Society 2013

Authors and Affiliations

  1. 1.School of Technology and HealthRoyal Institute of TechnologyHuddingeSweden

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