High Sensitivity Micro-Elastometry: Applications in Blood Coagulopathy
- First Online:
- 321 Downloads
Highly sensitive methods for the assessment of clot structure can aid in our understanding of coagulation disorders and their risk factors. Rapid and simple clot diagnostic systems are also needed for directing treatment in a broad spectrum of cardiovascular diseases. Here we demonstrate a method for micro-elastometry, named resonant acoustic spectroscopy with optical vibrometry (RASOV), which measures the clot elastic modulus (CEM) from the intrinsic resonant frequency of a clot inside a microwell. We observed a high correlation between the CEM of human blood measured by RASOV and a commercial thromboelastograph (TEG), (R = 0.966). Unlike TEG, RASOV requires only 150 μL of sample and offers improved repeatability. Since CEM is known to primarily depend upon fibrin content and network structure, we investigated the CEM of purified clots formed with varying amounts of fibrinogen and thrombin. We found that RASOV was sensitive to changes of fibrinogen content (0.5–6 mg/mL), as well as to the amount of fibrinogen converted to fibrin during clot formation. We then simulated plasma hypercoagulability via hyperfibrinogenemia by spiking whole blood to 150 and 200% of normal fibrinogen levels, and subsequently found that RASOV could detect hyperfibrinogenemia-induced changes in CEM and distinguish these conditions from normal blood.
KeywordsElastometry Coagulation Thromboelastography Fibrin Thrombosis Acoustic spectroscopy Hyperfibrinogenemia
- 12.International Commission on Radiation Units and Measurements (ICRU). Tissue substitutes in radiation dosimetry and measurement, Report 44. Bethesda, MD, 1989.Google Scholar
- 21.Scrutton, M. C., S. B. Ross-Murphy, G. M. Bennett, Y. Stirling, and T. W. Meade. Changes in clot deformability—a possible explanation for the epidemiological association between plasma fibrinogen concentration and myocardial infarction. Blood Coagul. Fibrinolysis 5:719–723, 1994.PubMedCrossRefGoogle Scholar