Annals of Biomedical Engineering

, Volume 41, Issue 10, pp 2120–2129

High Sensitivity Micro-Elastometry: Applications in Blood Coagulopathy

  • Gongting Wu
  • Charles R. Krebs
  • Feng-Chang Lin
  • Alisa S. Wolberg
  • Amy L. Oldenburg
Article

Abstract

Highly sensitive methods for the assessment of clot structure can aid in our understanding of coagulation disorders and their risk factors. Rapid and simple clot diagnostic systems are also needed for directing treatment in a broad spectrum of cardiovascular diseases. Here we demonstrate a method for micro-elastometry, named resonant acoustic spectroscopy with optical vibrometry (RASOV), which measures the clot elastic modulus (CEM) from the intrinsic resonant frequency of a clot inside a microwell. We observed a high correlation between the CEM of human blood measured by RASOV and a commercial thromboelastograph (TEG), (R = 0.966). Unlike TEG, RASOV requires only 150 μL of sample and offers improved repeatability. Since CEM is known to primarily depend upon fibrin content and network structure, we investigated the CEM of purified clots formed with varying amounts of fibrinogen and thrombin. We found that RASOV was sensitive to changes of fibrinogen content (0.5–6 mg/mL), as well as to the amount of fibrinogen converted to fibrin during clot formation. We then simulated plasma hypercoagulability via hyperfibrinogenemia by spiking whole blood to 150 and 200% of normal fibrinogen levels, and subsequently found that RASOV could detect hyperfibrinogenemia-induced changes in CEM and distinguish these conditions from normal blood.

Keywords

Elastometry Coagulation Thromboelastography Fibrin Thrombosis Acoustic spectroscopy Hyperfibrinogenemia 

Supplementary material

10439_2013_817_MOESM1_ESM.docx (29 kb)
Supplementary material 1 (DOCX 29 kb)

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Copyright information

© Biomedical Engineering Society 2013

Authors and Affiliations

  • Gongting Wu
    • 1
  • Charles R. Krebs
    • 2
  • Feng-Chang Lin
    • 3
  • Alisa S. Wolberg
    • 4
  • Amy L. Oldenburg
    • 1
    • 2
    • 5
  1. 1.Department of Physics and AstronomyUniversity of North Carolina at Chapel HillChapel HillUSA
  2. 2.Department of Biomedical EngineeringUniversity of North Carolina at Chapel HillChapel HillUSA
  3. 3.Department of BiostatisticsUniversity of North Carolina at Chapel HillChapel HillUSA
  4. 4.Department of Pathology and Laboratory MedicineUniversity of North Carolina at Chapel HillChapel HillUSA
  5. 5.Biomedical Research Imaging CenterUniversity of North Carolina at Chapel HillChapel HillUSA

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