Annals of Biomedical Engineering

, Volume 41, Issue 4, pp 682–693 | Cite as

Endothelial Dysfunction, Arterial Stiffening, and Intima-Media Thickening in Large Arteries from HIV-1 Transgenic Mice

  • Laura Hansen
  • Ivana Parker
  • Roy L. Sutliff
  • Manu O. Platt
  • Rudolph L. GleasonJr.Email author


HIV patients on highly active antiretroviral therapy (HAART) exhibit elevated incidence of cardiovascular disease (CVD), including a higher risk of myocardial infarction and prevalence of atherosclerotic lesions, as well as increases in markers of subclinical atherosclerosis including increased carotid artery intima-media thickness (c-IMT), increased arterial stiffness, and impaired flow-mediated dilation. Both HAART and HIV-infection are independent risk factors for atherosclerosis and myocardial infarction. Studies implicate the HIV proteins tat, gp120, vpu, and nef in early on-set atherosclerosis. The objective of this study was to quantify the role of expression of HIV-1 proteins on the vascular function, biomechanics, and geometry of common carotid arteries and aortas. This study employed NL4-3Δ gag/pol transgenic mice (HIV-Tg), which contain the genetic sequence for the HIV-1 proteins env, tat, nef, rev, vif, vpr, and vpu but lacks the gag and pol genes and reports that HIV-Tg mice have impaired aortic endothelial function, increased c-IMT, and increased arterial stiffness. Further, HIV-Tg arteries show decreased elastin content, increased cathepsin K and cathepsin S activity, and increased mechanical residual stress. Thus, mice that express HIV proteins exhibit pre-clinical markers of atherosclerosis and these markers correlate with changes in markers of vascular remodeling. These findings are consistent with the hypothesis that HIV-proteins, independent of HAART treatment or HIV infection, could play a role in of the development of CVD.


HIV Atherosclerosis Vascular remodeling 



We gratefully acknowledge the support from the National Institutes of Health and the International AIDS Society through the Creative and Novel Ideas in HIV Research (CNIHR) Program, the American Heart Association (11GRNT7990055), and the National Science Foundation Graduate Research Fellowship Program (LH).


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Copyright information

© Biomedical Engineering Society 2012

Authors and Affiliations

  • Laura Hansen
    • 1
  • Ivana Parker
    • 2
  • Roy L. Sutliff
    • 3
  • Manu O. Platt
    • 1
    • 4
  • Rudolph L. GleasonJr.
    • 1
    • 2
    • 4
    Email author
  1. 1.The Wallace H. Coulter Department of Biomedical EngineeringGeorgia Institute of TechnologyAtlantaUSA
  2. 2.The George W. Woodruff School of Mechanical EngineeringGeorgia Institute of TechnologyAtlantaUSA
  3. 3.Department MedicineEmory University/Atlanta VAMCAtlantaUSA
  4. 4.The Petit Institute for Bioengineering and BiosciencesGeorgia Institute of TechnologyAtlantaUSA

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