Annals of Biomedical Engineering

, Volume 40, Issue 2, pp 277–291

Two-Photon and Second Harmonic Microscopy in Clinical and Translational Cancer Research


DOI: 10.1007/s10439-012-0512-9

Cite this article as:
Perry, S.W., Burke, R.M. & Brown, E.B. Ann Biomed Eng (2012) 40: 277. doi:10.1007/s10439-012-0512-9


Application of two-photon microscopy (TPM) to translational and clinical cancer research has burgeoned over the last several years, as several avenues of pre-clinical research have come to fruition. In this review, we focus on two forms of TPM—two-photon excitation fluorescence microscopy, and second harmonic generation microscopy—as they have been used for investigating cancer pathology in ex vivo and in vivo human tissue. We begin with discussion of two-photon theory and instrumentation particularly as applicable to cancer research, followed by an overview of some of the relevant cancer research literature in areas that include two-photon imaging of human tissue biopsies, human skin in vivo, and the rapidly developing technology of two-photon microendoscopy. We believe these and other evolving two-photon methodologies will continue to help translate cancer research from the bench to the bedside, and ultimately bring minimally invasive methods for cancer diagnosis and treatment to therapeutic reality.


Two-photon microscopy Cancer Second harmonic generation Collagen SHG Endoscopy 

Copyright information

© Biomedical Engineering Society 2012

Authors and Affiliations

  • Seth W. Perry
    • 1
    • 2
  • Ryan M. Burke
    • 1
  • Edward B. Brown
    • 1
  1. 1.Department of Biomedical EngineeringUniversity of RochesterRochesterUSA
  2. 2.Department of NeurologyUniversity of Rochester School of Medicine and DentistryRochesterUSA

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