Local Strain Distribution in Real Three-Dimensional Alveolar Geometries
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Mechanical ventilation is not only a life saving treatment but can also cause negative side effects. One of the main complications is inflammation caused by overstretching of the alveolar tissue. Previously, studies investigated either global strains or looked into which states lead to inflammatory reactions in cell cultures. However, the connection between the global deformation, of a tissue strip or the whole organ, and the strains reaching the single cells lining the alveolar walls is unknown and respective studies are still missing. The main reason for this is most likely the complex, sponge-like alveolar geometry, whose three-dimensional details have been unknown until recently. Utilizing synchrotron-based X-ray tomographic microscopy, we were able to generate real and detailed three-dimensional alveolar geometries on which we have performed finite-element simulations. This allowed us to determine, for the first time, a three-dimensional strain state within the alveolar wall. Briefly, precision-cut lung slices, prepared from isolated rat lungs, were scanned and segmented to provide a three-dimensional geometry. This was then discretized using newly developed tetrahedral elements. The main conclusions of this study are that the local strain in the alveolar wall can reach a multiple of the value of the global strain, for our simulations up to four times as high and that thin structures obviously cause hotspots that are especially at risk of overstretching.
KeywordsAlveoli Finite-element method Local strains Synchrotron-based X-ray tomographic microscopy
Support by the German Science Foundation/Deutsche Forschungsgemeinschaft DFG and the TUM Graduate School is gratefully acknowledged. We also gratefully acknowledge the help of Christian Martin and Stefan Uhlig for providing us with the PCLSs.
- 3.Chandel, N. S., and J. I. Sznajder. Stretching the lung and programmed cell death. Am. J. Physiol. Lung Cell Mol. Physiol. 279(6):1003–1004, 2000.Google Scholar
- 14.Holzapfel, G. A. Nonlinear Solid Mechanics: A Continuum Approach for Engineering. New York: Wiley, 2001.Google Scholar
- 22.Schittny, J. C., and P. H. Burri. Development and Growth of the Lung. Fishman’s Pulmonary Diseases and Disorders. New-York: McGraw-Hill, 2008.Google Scholar
- 25.Stampanoni, M., A. Groso, A. Isenegger, G. Mikuljan, Q. Chen, A. Bertrand, S. Henein, R. Betemps, U. Frommherz, P. Böhler, D. Meister, M. Lange, and R. Abela. Trends in synchrotron-based tomographic imaging: the SLS experience. In: Society of Photo-Optical Instrumentation Engineers (SPIE) Conference Series, 2006.Google Scholar
- 30.Vlahakis, N. E., M. A. Schroeder, A. H. Limper, and R. D. Hubmayr. Stretch induces cytokine release by alveolar epithelial cells in vitro. Am. J. Physiol. 277(1):167–173, 1999.Google Scholar
- 31.Wall, W. A., and M. Gee. Baci: A Parallel Multiphysics Simulation Environment. Technical Report, Institute for Computational Mechanics, TUM, 2010.Google Scholar
- 32.Wall, W. A., L. Wiechert, A. Comerford, and S. Rausch. Towards a comprehensive computational model for the respiratory system. Int. J. Numer. Methods Biomed. Eng. 26(7):807–827, 2010.Google Scholar