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Annals of Biomedical Engineering

, Volume 38, Issue 6, pp 1965–1976 | Cite as

Peptide Interfacial Biomaterials Improve Endothelial Cell Adhesion and Spreading on Synthetic Polyglycolic Acid Materials

  • Xin Huang
  • Stefan Zauscher
  • Bruce Klitzman
  • George A. Truskey
  • William M. Reichert
  • Daniel J. Kenan
  • Mark W. Grinstaff
Article

Abstract

Resorbable scaffolds such as polyglycolic acid (PGA) are employed in a number of clinical and tissue engineering applications owing to their desirable property of allowing remodeling to form native tissue over time. However, native PGA does not promote endothelial cell adhesion. Here we describe a novel treatment with hetero-bifunctional peptide linkers, termed “interfacial biomaterials” (IFBMs), which are used to alter the surface of PGA to provide appropriate biological cues. IFBMs couple an affinity peptide for the material with a biologically active peptide that promotes desired cellular responses. One such PGA affinity peptide was coupled to the integrin binding domain, Arg-Gly-Asp (RGD), to build a chemically synthesized bimodular 27 amino acid peptide that mediated interactions between PGA and integrin receptors on endothelial cells. Quartz crystal microbalance with dissipation monitoring (QCMD) was used to determine the association constant (K A 1 × 107 M−1) and surface thickness (~3.5 nm). Cell binding studies indicated that IFBM efficiently mediated adhesion, spreading, and cytoskeletal organization of endothelial cells on PGA in an integrin-dependent manner. We show that the IFBM peptide promotes a 200% increase in endothelial cell binding to PGA as well as 70–120% increase in cell spreading from 30 to 60 minutes after plating.

Keywords

PGA Surface modification IFBM Peptides RGD Biomaterials Scaffolds 

Notes

Acknowledgments

This work was supported by the National Institutes of Health (Grant 5R01EB000501 to MWG) and the North Carolina Biotechnology Center (Collaborative Funding Grant to DJK supporting XH). The authors thank Felix Yap for assistance with image analysis, and Erin Carruthers, PhD, for assistance with QCM-D.

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Copyright information

© Biomedical Engineering Society 2010

Authors and Affiliations

  • Xin Huang
    • 1
  • Stefan Zauscher
    • 2
  • Bruce Klitzman
    • 1
    • 3
  • George A. Truskey
    • 1
  • William M. Reichert
    • 1
  • Daniel J. Kenan
    • 4
  • Mark W. Grinstaff
    • 5
  1. 1.Department of Biomedical EngineeringDuke UniversityDurhamUSA
  2. 2.Department of Mechanical Engineering and Material ScienceDuke UniversityDurhamUSA
  3. 3.Kenan Plastic Surgery Research LabsDuke University Medical CenterDurhamUSA
  4. 4.Department of PathologyDuke University Medical CenterDurhamUSA
  5. 5.Departments of Biomedical Engineering and ChemistryBoston UniversityBostonUSA

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