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Annals of Biomedical Engineering

, Volume 38, Issue 3, pp 1164–1177 | Cite as

Microfluidic Platforms for Studies of Angiogenesis, Cell Migration, and Cell–Cell Interactions

Sixth International Bio-Fluid Mechanics Symposium and Workshop March 28–30, 2008 Pasadena, California
  • Seok Chung
  • Ryo Sudo
  • Vernella Vickerman
  • Ioannis K. Zervantonakis
  • Roger D. Kamm
Position Paper

Abstract

Recent advances in microfluidic technologies have opened the door for creating more realistic in vitro cell culture methods that replicate many aspects of the true in vivo microenvironment. These new designs (i) provide enormous flexibility in controlling the critical biochemical and biomechanical factors that influence cell behavior, (ii) allow for the introduction of multiple cell types in a single system, (iii) provide for the establishment of biochemical gradients in two- or three-dimensional geometries, and (iv) allow for high quality, time-lapse imaging. Here, some of the recent developments are reviewed, with a focus on studies from our own laboratory in three separate areas: angiogenesis, cell migration in the context of tumor cell-endothelial interactions, and liver tissue engineering.

Keywords

Cell culture Cancer Tissue engineering Liver Vascular networks 

Abbreviations

2D

Two-dimensional

3D

Three-dimensional

ECIS

Electric cell-substrate impedance sensing

ECM

Extracellular matrix

EC

Endothelial cell

SEC

Sinusoidal endothelial cell

EGF

Epidermal growth factor

hMVEC

Human microvascular endothelial cells

HSC

Hepatic stellate cell

PDMS

Polydimethylsiloxane

VEGF

Vascular endothelial growth factor

Notes

Acknowledgments

The authors would like to express their gratitude to Draper Laboratories (IR&D Project N. DL-H-550151), the National Science Foundation (EFRI-0735997), the NHLBI (EB003805), and the Singapore-MIT Alliance for Research and Technology.

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Copyright information

© Biomedical Engineering Society 2010

Authors and Affiliations

  • Seok Chung
    • 1
  • Ryo Sudo
    • 2
  • Vernella Vickerman
    • 3
  • Ioannis K. Zervantonakis
    • 4
  • Roger D. Kamm
    • 4
    • 5
  1. 1.School of Mechanical EngineeringKorea UniversitySeoulKorea
  2. 2.Department of System Design EngineeringKeio UniversityYokohamaJapan
  3. 3.Department of Chemical EngineeringMassachusetts Institute of TechnologyCambridgeUSA
  4. 4.Department of Mechanical EngineeringMassachusetts Institute of TechnologyCambridgeUSA
  5. 5.Department of Biological EngineeringMassachusetts Institute of TechnologyCambridgeUSA

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