Annals of Biomedical Engineering

, Volume 38, Issue 11, pp 3440–3448

A Parallel Excitation Based Fluorescence Molecular Tomography System for Whole-Body Simultaneous Imaging of Small Animals

Article

DOI: 10.1007/s10439-010-0093-4

Cite this article as:
Liu, F., Liu, X., Wang, D. et al. Ann Biomed Eng (2010) 38: 3440. doi:10.1007/s10439-010-0093-4

Abstract

Challenges remain in imaging complete dynamic physiological processes in vivo through the whole small animal body using fluorescence molecular tomography (FMT). In this article, a novel non-contact full-angle FMT system that enables whole-body simultaneous imaging of small animals is presented. The whole-body simultaneous imaging ability is achieved by employing a line-shaped parallel excitation source, which can provide extended spatial sampling dataset to reconstruct multiple fluorescent targets distributed in whole animal body during one full-angle FMT imaging process. The key performances of this system were evaluated by a series of experiments. Quantitation linearity for over two orders of magnitude of fluorescence markers concentration was demonstrated, and an accessible simultaneous imaging domain of 4.0 × 1.5 cm2 could be achieved utilizing the parallel excitation pattern. Moreover, the in vivo imaging feasibility and performance were validated by localizing two fluorescent targets implanted at different positions of a nude mouse. The results suggest that compared with conventional single point excitation FMT system, the proposed system can achieve a whole-body simultaneous imaging domain and impart the ability to image complete dynamic physiological processes in vivo.

Keywords

Fluorescence molecular tomography Image reconstruction techniques Medical and biological imaging 

Copyright information

© Biomedical Engineering Society 2010

Authors and Affiliations

  • Fei Liu
    • 1
  • Xin Liu
    • 1
  • Daifa Wang
    • 1
  • Bin Zhang
    • 1
  • Jing Bai
    • 1
  1. 1.Department of Biomedical Engineering, School of MedicineTsinghua UniversityBeijingChina

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