Fusion of Concentrically Layered Tubular Tissue Constructs Increases Burst Strength
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Tubular tissue constructs prepared from neonatal human dermal fibroblasts entrapped in fibrin gel were incubated on a mandrel for three weeks to allow for initial fibrin remodeling into tissue before being concentrically layered and incubated for an additional three weeks on the mandrel. Upon harvest, double layer constructs were not statistically different from single layer control constructs in terms of length, collagen density, cell density, tensile modulus, or ultimate tensile strength. However, the thickness and burst pressure were both approximately twice the single layer control values. Metabolically active cells were detected at the interface, and scanning electron microscopy revealed fiber structures bridging the two layers, co-localizing with the cells, which exhibited minimal migration across the layers. In contrast, double layer constructs where tissue fusion was prohibited by mechanical distraction of the layers showed no increase in burst pressure despite having increased thickness and the same collagen and cell densities of the single layer control constructs; moreover, the burst failure occurred sequentially in the layers in contrast to simultaneous failure for the fused double layer constructs. This study provides insight into the nature of the interface and the role of cell behavior when tissue fusion occurs between two layers of bioartificial tissue in vitro. It also suggests a method for improving the burst strength of fibrin-based tubular tissue constructs by increasing the construct thickness via concentrically layering and fusing two constructs.
KeywordsTissue engineering Vascular engineering Tissue-engineered vascular graft Tissue-engineered blood vessel Tissue fusion
- 1.American Heart Association. Cardiovascular Disease Statistics, 2009. www.americanheart.org/downloadable/heart/1240250946756LS-1982%20Heart%20and%20Stroke%20Update.042009.pdf.
- 3.Auger, F. A., M. Remy-Zolghadri, G. Grenier, and L. Germain. A truly new approach for tissue engineering: the LOEX self-assembly technique. Ernst Schering Res. Found. Workshop 35:73–88, 2002.Google Scholar
- 6.Fung, Y. C. Biomechanics: Mechanical Properties of Living Tissues. New York: Springer, pp. 2–22, 1993.Google Scholar
- 10.Hansen, M. E., K. Yucel, J. Megerman, G. J. L’Italien, W. M. Abbott, and A. C. Waltmaff. In vivo determination of human arterial compliance: preliminary investigation of a new technique. Cardiovasc. Intervent. Radiol. 17:22–26, 1994.Google Scholar
- 15.Jakab, K., C. Norotte, B. Damon, F. Marga, A. Neagu, C. L. Besch-Williford, A. Kachurin, K. H. Church, H. Park, V. Mironov, R. Markwald, G. Vunjak-Novakovic, and G. Forgacs. Tissue engineering by self-assembly of cells printed into topologically defined structures. Tissue Eng. Part A 14:413–421, 2008.CrossRefPubMedGoogle Scholar
- 17.Konig, G., T. McAllister, N. Dusserre, S. Garrido, C. Iyican, A. Marini, A. Fiorillo, H. Avila, W. Wystrychowski, K. Zagalski, M. Maruszewski, A. Jones, L. Cierpka, L. de la Fuente, and N. L’Heureux. Mechanical properties of completely autologous human tissue engineered blood vessels compared to human saphenous vein and mammary artery. Biomaterials 30(8):1542–1550, 2009 (Epub 2008 Dec 25).CrossRefPubMedGoogle Scholar
- 23.McAllister, T. N., M. Maruszewski, S. A. Garrido, W. Wystrychowski, N. Dusserre, A. Marini, K. Zagalski, A. Fiorillo, H. Avila, X. Manglano, J. Antonelli, A. Kocher, M. Zembala, L. Cierpka, L. M. de la Fuente, and N. L’Heureux. Effectiveness of haemodialysis access with an autologous tissue-engineered vascular graft: a multicentre cohort study. Lancet 373:1440–1446, 2009.CrossRefPubMedGoogle Scholar
- 27.Nieponice, A., L. Soletti, J. Guan, B. M. Deasy, J. Huard, W. R. Wagner, and D. A. Vorp. Development of a tissue-engineered vascular graft combining a biodegradable scaffold, muscle-derived stem cells and a rotational vacuum seeding technique. Biomaterials 29:825–833, 2008.Google Scholar
- 28.O’Cearbhaill, E., M. Murphy, F. Barry, P. McHugh, and V. Barron. Behavior of human mesenchymal stem cells in fibrin-based vascular tissue engineering constructs. Ann. Biomed. Eng., 2010 (Epub ahead of Print).Google Scholar
- 37.Tschoeke, B., T. C. Flanagan, M. Harwoko, S. Koch, T. Deichmann, V. Ellå, J. S. Sachweh, M. Kellomåki, T. Gries, T. Schmitz-Rode, and S. Jockenhoevel. Tissue-engineered small-caliber vascular graft based on a novel biodegradable composite fibrin-polylactide scaffold. Tissue Eng. Part A 15(8):1909–1918, 2009.Google Scholar