Effects of Hydroxyapatite and Biostite® on Osteogenic Induction of hMSC
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When isolated from the iliac crest human mesenchymal stem cells (hMSC) differentiate into osteoblast-like cells with appropriate stimulation in culture. This in vitro study tested the hypothesis that Biostite® and hydroxyapatite (HA) affect proliferation and differentiation of hMSC into osteoblastic cells. Cell proliferation was determined by measuring 3H-thymidine incorporation into DNA and typical markers of osteoblastic phenotype were determined by RT-PCR assay. No differences emerged in cell proliferation cultures with Biostite® or hydroxyapatite (HA), but gene expression analysis revealed higher expression of collagen, alkaline phosphatase (ALP), osteopontin and bone sialoprotein (BSP) in the presence of Biostite®. TGFβ2 production, as assessed by an Elisa kit, and Runx2 expression by RT-PCR, were greater in Biostite cultures, suggesting Biostite® provides a better environment for hMSC differentiation into osteoblasts and is, potentially, a more promising bone-filling material than HA.
KeywordsBiomaterials Osteoblastic differentiation Extracellular matrix production TGFβ2
We thank Dr. G.A. Boyd for help with the English translation. Moreover we thank the Fondazione Cassa di Risparmio of Perugia for providing the instruments for in vitro experiments.
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