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Annals of Biomedical Engineering

, Volume 38, Issue 4, pp 1399–1414 | Cite as

Incorporating Histology into a 3D Microscopic Computer Model of Myocardium to Study Propagation at a Cellular Level

  • Jeroen Stinstra
  • Rob MacLeod
  • Craig Henriquez
Article

Abstract

We introduce a 3D model of cardiac tissue to study at a microscopic level the relationship between tissue morphology and propagation of depolarization. Unlike the classical bidomain approach, in which tissue properties are described by the apparent conductivity of the tissue, in this “microdomain” approach, we included histology by modeling the actual shape of the intracellular and extracellular spaces that contain spatially distributed gap-junctions and membranes. The histological model of the tissue was generated by a computer algorithm that can be tuned to model different histological changes. For healthy tissue, the model predicted a realistic conduction velocity of 0.42 m/s based solely on the parameters derived from histology. A comparison with a brick-shaped, simplified model showed that conduction depended to a moderate extent on the shape of myocytes; a comparison with a one-dimensional bidomain model with the same overall shape and structure showed that the apparent conductivity of the tissue can be used to create an equivalent bidomain model. In summary, the microdomain approach offers a means of directly incorporating structural and functional parameters into models of cardiac activation and propagation and thus provides a valuable bridge between the cellular and tissue domains in the myocardium.

Keywords

Cardiac tissue Computer model Propagation Simulation 

Notes

Acknowledgments

We acknowledge support for this work through NIH grants RO1 HL076767 and P41-RR12553-07 as well as the Nora Eccles Treadwell Foundation.

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Copyright information

© Biomedical Engineering Society 2009

Authors and Affiliations

  1. 1.Scientific Computing and Imaging InstituteUniversity of UtahSalt Lake CityUSA
  2. 2.Scientific Computing and Imaging Institute, Department of BioengineeringUniversity of UtahSalt Lake CityUSA
  3. 3.Department of BioengineeringDuke UniversityDurhamUSA

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