High-resolution Mapping of In Vivo Gastrointestinal Slow Wave Activity Using Flexible Printed Circuit Board Electrodes: Methodology and Validation
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High-resolution, multi-electrode mapping is providing valuable new insights into the origin, propagation, and abnormalities of gastrointestinal (GI) slow wave activity. Construction of high-resolution mapping arrays has previously been a costly and time-consuming endeavor, and existing arrays are not well suited for human research as they cannot be reliably and repeatedly sterilized. The design and fabrication of a new flexible printed circuit board (PCB) multi-electrode array that is suitable for GI mapping is presented, together with its in vivo validation in a porcine model. A modified methodology for characterizing slow waves and forming spatiotemporal activation maps showing slow waves propagation is also demonstrated. The validation study found that flexible PCB electrode arrays are able to reliably record gastric slow wave activity with signal quality near that achieved by traditional epoxy resin-embedded silver electrode arrays. Flexible PCB electrode arrays provide a clinically viable alternative to previously published devices for the high-resolution mapping of GI slow wave activity. PCBs may be mass-produced at low cost, and are easily sterilized and potentially disposable, making them ideally suited to intra-operative human use.
KeywordsPCB Gastric electrical activity Smooth muscle Activation map Velocity
This work is partially supported by Grants from the NIH (R01 DK64775), NZ Society of Gastroenterology, the NZ Health Research Council and the Auckland Medical Research Foundation. We thank Linley Nisbett for her assistance with the validation studies in this report.
- 9.Cheng, L. K., G. O’Grady, P. Du, J. U. Egbuji, J. A. Windsor, and A. J. Pullan. Gastrointestinal system. Wiley Interdiscip. Rev.: Syst. Biol. Med., 2009, in press. doi: 10.1002/wnan.019.
- 10.Chou CC, Zhou S, Tan AY, Hayashi H, Nihei M, and Chen PS. High-density mapping of pulmonary veins and left atrium during ibutilide administration in a canine model ofsustained atrial fibrillation. Am J Physiol Heart Circ Physiol 289: H2704–2713, 2005. doi: 10.1152/ajpheart.00537.2005 PubMedCrossRefGoogle Scholar
- 23.Lammers, W. J., L. Ver Donck, B. Stephen, D. Smets, and J. A. Schuurkes. Focal activities and re-entrant propagations as mechanisms of gastric tachyarrhythmias. Gastroenterology 135:1601–1611, 2008.Google Scholar
- 29.Ordog T, Redelman D, Horvath VJ, Miller LJ, Horowitz B, and Sanders KM. Quantitative analysis by flow cytometry of interstitial cells of Cajal, pacemakers, and mediators of neurotransmission in the gastrointestinal tract. Cytometry A 62: 139–149, 2004. doi: 10.1002/cyto.a.20078 PubMedCrossRefGoogle Scholar
- 30.Shenasa M, Borggrefe M, and Breithardt G. Cardiac Mapping. New York: Futura Press, 2003.Google Scholar
- 32.Sih HJ and Berbari EJ. Chapter 3: Methodology of Cardiac Mapping. In Cardiac Mapping. New York: Futura Press, 2003.Google Scholar
- 35.Yang, C., Z. Fang, X. Wu, A. Lou, and J. Lu. Dynamic 3D epicardial mapping of whole-atrium. In: World Congress on Medical Physics and Biomedical Engineering. Berlin, Heidelberg: Springer, 2007, pp. 894–897.Google Scholar
- 36.Zhou S, Chang CM, Wu TJ, Miyauchi Y, Okuyama Y, Park AM, Hamabe A, Omichi C, Hayashi H, Brodsky LA, Mandel WJ, Ting CT, Fishbein MC, Karagueuzian HS, and Chen PS. Nonreentrant focal activations in pulmonary veins in canine model of sustained atrial fibrillation. Am J Physiol Heart Circ Physiol 283: H1244–1252, 2002.PubMedGoogle Scholar
- 37.Zhou, T., W. Lu, C. Yang, and Z. Fang. A visual expression to show epicardial electrical activity comprehensively. In: Bioinformatics and Biomedical Engineering, 2008. ICBBE 2008. The 2nd International Conference on 16–18 May 2008, pp. 808–811.Google Scholar