Novel Organotypic Cultures of Human Skin Explants with an Implant-tissue Biomaterial Interface
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A novel in vitro culture system of organotypic human skin explants interfacing with external fixator pins is presented. The system was used to observe changes in skin morphology on the skin at the pin interface. To evaluate the performance of this novel system, histological analysis of human skin explants cultured for 5 days at the air–liquid interface was performed. Compared to control explants, specimens interfaced with pins (treated or not with a physiological saline solution) showed a deteriorating basal layer, a disappearing stratum spinosum and increased lost of elastic fibers in the dermis. The model system makes it possible to perform rapid, repeatable studies of living skin response to chronically implanted materials and devices.
KeywordsOrganotypic culture Wound healing Skin biomaterial Implant interface External fixation
We thank Harald Eberhart, College of Engineering, University of Michigan, for help with the glass lid design. We thank Marta Dzaman, Morphology Core, University of Michigan for advice on specimen sectioning and staining. This report is presented as part of the research efforts within the Army Research Office Multidisciplinary University Research Initiative award on Bio-Integrating Structural and Neural Prosthetic Materials and we gratefully acknowledge the funding provided.
- 4.Campbell A. A., L. Song, X. S. Li, B. J. Nelson, C. Bottoni, D. E. Brooks, E. S. DeJong. Development, characterization, and anti-microbial efficacy of hydroxyapatite-chlorhexidine coatings produced by surface-induced mineralization. J. Biomed. Mater. Res. (Appl. Biomater.) 2000;53:400–407CrossRefGoogle Scholar
- 11.Moroni A., V. L. Caja, C. Maltarello. Enhancement of the bone fixation external pin interface: a biomechanical and morphological in vivo experimental study. Bioceram 1994;7:229–234Google Scholar
- 14.Peramo, A., C. Marcelo, S. Goldstein, and D. C. Martin. Novel In Vitro Tissue Culture System for Interfacing Biological Tissues to Implanted Devices. Invention Disclosure, University of Michigan Technology Transfer Office, 2007Google Scholar
- 26.Von Eiff C., W. Kohnen, K. Becker, B. Jansen. Modern strategies in the prevention of implant-associated infections. Int. J. Artif. Organs 2005;28:1146–1156Google Scholar
- 29.Xu W., D. H. Xu, D. A. Crocombe. Three-dimensional finite element stress and strain analysis of a transfemoral osseointegration implant. Proc. Inst. Mech. Eng. [H] 2006;220:661–670Google Scholar