Novel Organotypic Cultures of Human Skin Explants with an Implant-tissue Biomaterial Interface
A novel in vitro culture system of organotypic human skin explants interfacing with external fixator pins is presented. The system was used to observe changes in skin morphology on the skin at the pin interface. To evaluate the performance of this novel system, histological analysis of human skin explants cultured for 5 days at the air–liquid interface was performed. Compared to control explants, specimens interfaced with pins (treated or not with a physiological saline solution) showed a deteriorating basal layer, a disappearing stratum spinosum and increased lost of elastic fibers in the dermis. The model system makes it possible to perform rapid, repeatable studies of living skin response to chronically implanted materials and devices.
KeywordsOrganotypic culture Wound healing Skin biomaterial Implant interface External fixation
We thank Harald Eberhart, College of Engineering, University of Michigan, for help with the glass lid design. We thank Marta Dzaman, Morphology Core, University of Michigan for advice on specimen sectioning and staining. This report is presented as part of the research efforts within the Army Research Office Multidisciplinary University Research Initiative award on Bio-Integrating Structural and Neural Prosthetic Materials and we gratefully acknowledge the funding provided.
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