Applied Biochemistry and Microbiology

, Volume 41, Issue 2, pp 199–204 | Cite as

The use of liposomes for detection of the surface lipopolysaccharide antigen, Vibrio cholerae cells, and antibodies against them

  • S. N. Skopinskaya
  • S. P. Yarkov
  • E. N. Chramov
Article
Received:

Abstract

A test system for determination of Vibrio cholerae cells, surface O-antigen, and antibodies against them was developed on the basis of complement-dependent lysis of liposomes sensitized by the lipopolysaccharide antigen from Vibrio cholerae 569B. The factors that affect the function of the liposomal reagent were studied, and the conditions for detecting antibodies and antigenic material were optimized. This system is highly specific and sensitive to use for the determination of anticholeraic antibodies (30–50 times as effective as agglutination tests), lipopolysaccharide antigen (100 ng/ml, which corresponded to 3.0 ng of lipopolysaccharide in the sample studied), and Vibrio cholerae cells (3.3 × 107 m.b./ml, which corresponded to 106 m.b. in sample). It takes 30–40 min to detect the lipopolysaccharide antigen and antibodies and 90 min to detect V. cholerae cells.

Keywords

Vibrio Agglutination Test Antigenic Material Cholerae Cell Lipopolysaccharide Antigen 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© MAIK “Nauka/Interperiodica” 2005

Authors and Affiliations

  • S. N. Skopinskaya
    • 1
  • S. P. Yarkov
    • 1
  • E. N. Chramov
    • 1
  1. 1.State Research Institute of Biological Instrument DesignMoscowRussia

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