Oil-oxidizing bacteria were isolated from oil-polluted soil and water samples and identified as Acinetobacter calcoaceticus K-4, Nocardia vaceinii K-8, Rhodococcus erythropolis EK-1, and Mycobacterium sp. K-2. It was found that immobilization of the bacteria on an expanded clay aggregate accelerated their growth and consumption of hydrocarbon substrates. It was also found that water polluted with 100 mg/l oil could be purified with Rhodococcus erythropolis EK-1 and Nocardia vaceinii K-8 cells immobilized in this way. The dependence of the degree of water purification on its flow rate, aeration, and availability of nitrogen and phosphorus sources was determined. The efficiency of water purification from oil by immobilized Rhodococcus erythropolis EK-1 cells at high flow rates (of up to 0.68 l/h), low aeration (of 0.1 l/l per min) and an intermittent supply of 0.01% diammonium phosphate reached 99.5–99.8%.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Bitteeva, M.B., Murzakov, B.G., Morshchakova, G.N., Khamroev, O.Zh., Kapotina, L.N., Fadeeva, T.N., Pomazkova, V.A., Levandovskaya, Yu.B., Demidova, E.I., and Pospelov, M.E., RF Patent no. 2014286, 1994.
Kozhanova, G.A., RF Patent no. 2031860, 1995.
Murzakov, B.G., Morshchakova, G.N., and Kapotkina, L.N., RF Patent no. 2053204, 1996.
Ogurtsova, L.V., Morozova, T.N., and Zhdanova, E.V., RF Patent no. 2129604, 1999.
G.G. Yagafarova A.Kh. Safarov E.G. Il’ina I.R. Yagafarov V.B. Barakhnina M.E. Sukharevich (2002) Prikl. Biokhim. Mikrobiol. 38 IssueID5 518–522
F. Gerhardt (Eds) (1984) Metody obshchei bakteriologii Mir Moscow
J. Holt (Eds) (1997) Bergey’s Manual of Determinative Bacteriology Williams and Wilkins Baltimore
T. Kodama T. Nakakhara T. Omori N.T. Binkh K. Khashino I. Minoda (1977) Rost mikroorganizmov na S1-soedineniyakh. Tez. dokl. Simp. NTsBI AN SSSR Pushchino 213–215
S.J. Pirt (1975) Principles of Microbe and Cell Cultivation Blackwell Oxford
A.I. Iz”yurov (1987) Gig. Sanit. 6 IssueID5 15
D.L. Gutnick G.S. Wise (1987) Biopolymers 26 223–240
R. Atlas R. Bartha (1972) Biotechnol. Bioeng. 14 IssueID3 309
Translated from Prikladnaya Biokhimiya i Mikrobiologiya, Vol. 41, No. 1, 2005, pp. 58–63.
Original Russian Text Copyright © 2005 by Pirog, Shevchuk, Voloshina, Gregirchak.
About this article
Cite this article
Pirog, T.P., Shevchuk, T.A., Voloshina, I.N. et al. Use of claydite-immobilized oil-oxidizing microbial cells for purification of water from oil. Appl Biochem Microbiol 41, 51–55 (2005). https://doi.org/10.1007/s10438-005-0010-z
- Water Purification