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Fabrication of three-dimensional islet models by the geometry-controlled hanging-drop method

  • Bin Gao
  • Ce Jing
  • Kelvin Ng
  • Belinda Pingguan-Murphy
  • Qingzhen YangEmail author
Research Paper

Abstract

The hanging-drop method has been widely used to fabricate three-dimensional (3D) in vitro tissue models due to its advantages such as being easy to perform, inexpensive, and permitting precise control of cell spheroid formation. The geometry of hanging drop may play a critical role on the formation of cell spheroids, which, however, has not been explored. In this study, we developed a modified hanging-drop platform that enables the production of cell spheroids in a high-throughput manner by controlling hanging drop geometry with a defined spreading ring. The surface tension force is proportional to the spreading ring and the gravitational force is determined by droplet volume, and the geometry can be determined by the balance between surface tension and gravity. β-TC-6 cell spheroids with optimized diameters were fabricated as 3D in vitro islet models. The models showed morphology similar to primary islets and had functionality that more closely resembled primary islets than two-dimensional cell culture. The developed platform holds great potential for engineering well-controlled in vitro tissue models for various applications such as physiological and pathological studies, drug screening, as well as transplantation for treatment purpose.

Keywords

Hanging drop cell culture Mechanical microenvironment Drop geometry 3D islet spheroids 

Notes

Acknowledgements

This work was financially supported by the National Natural Science Foundation of China (Grants 51605377 and 81300696) and the Natural Science Foundation of Shaanxi Province (Grant 2017JQ5004).

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Copyright information

© The Chinese Society of Theoretical and Applied Mechanics and Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Bin Gao
    • 1
  • Ce Jing
    • 2
  • Kelvin Ng
    • 3
  • Belinda Pingguan-Murphy
    • 3
  • Qingzhen Yang
    • 4
    • 5
    Email author
  1. 1.Department of Endocrinology and Metabolism, The First Affiliated HospitalAir Force Military Medical UniversityXi’anChina
  2. 2.Outpatient Department of Lanzhou Military Region, 940 HospitalJoint Logistics Support Force of the People’s Liberation Army of ChinaLanzhouChina
  3. 3.Department of Biomedical Engineering, Faculty of EngineeringUniversity of MalayaKuala LumpurMalaysia
  4. 4.Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and TechnologyXi’an Jiaotong UniversityXi’anChina
  5. 5.Bioinspired Engineering and Biomechanics Center (BEBC)Xi’an Jiaotong UniversityXi’anChina

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