Advertisement

On-board control of wax valve on active centrifugal microfluidic chip and its application for plasmid DNA extraction

  • Yihui Wang
  • Zhongwen Li
  • Xinyu Huang
  • Wenbin Ji
  • Xinghai Ning
  • Kangkang Liu
  • Jie Tan
  • Jiachen Yang
  • Ho-pui Ho
  • Guanghui WangEmail author
Research Paper
  • 78 Downloads

Abstract

For the realization of bioassay with complex fluidic manipulation and logic operation on lab-on-a-disc platform, we present an active integrated centrifugal microfluidic chip based on the on-board control of wax valves within a multilayer complex chip. The multilayer hybrid structure including a microfluidic layer and a printing circuit board (PCB) layer utilizes the digital logic of electronic system to control the logic of liquid flow in microfluidic layer. The coupling mechanism between both layers is based on heat transfer, namely, the heating resistors in PCB layer are used to melt and open the paraffin wax valves in microfluidic layer. Without the limitation of surface tension-dependent valves, the application of active valve could be freely designed, which can largely extend the ability of integration on microfluidic chip. Many complex functional units including liquid sequential loading and switching of liquid flow are demonstrated. As an application, we also present a multilayer complex chip for plasmid DNA extraction based on our platform. In a word, our active centrifugal microfluidic platform provides a solution for the integration of complex bioassay on rotating disc, which has great potential in the applications of point-of-care diagnostics (POC).

Keywords

Active centrifugal microfluidics Paraffin wax valve Multilayer complex chip Lab-on-a-disc DNA extraction 

Notes

Acknowledgements

This work was sponsored by National Key Technologies R&D Program of China (2016YFC0800502), National Natural Science Foundation of China (nos. 61875083, 61535005) and Natural Science Foundation of Jiangsu Province (BK20180328).

Supplementary material

10404_2019_2278_MOESM1_ESM.mp4 (12.1 mb)
Supplementary material 1 (MP4 12406 kb)

References

  1. Al-Faqheri W, Ibrahim F, Thio TH, Moebius J, Joseph K, Arof H, Madou M (2013) Vacuum/compression valving (VCV) using paraffin-wax on a centrifugal microfluidic CD platform. PLoS One 8:e58523CrossRefGoogle Scholar
  2. Amasia M, Cozzens M, Madou MJ (2012) Centrifugal microfluidic platform for rapid PCR amplification using integrated thermoelectric heating and ice-valving. Sens Actuators B Chem 161:1191–1197CrossRefGoogle Scholar
  3. Chang N, Zhai J, Liu B, Zhou J, Zeng Z, Zhao X (2018) Low cost 3D microfluidic chips for multiplex protein detection based on photonic crystal beads. Lab Chip 18:3638–3644.  https://doi.org/10.1039/c8lc00784e CrossRefGoogle Scholar
  4. Chin CD, Linder V, Sia SK (2006) Lab-on-a-chip devices for global health: past studies and future opportunities. Lab Chip 7:41–57CrossRefGoogle Scholar
  5. Daniel M, Stefan H, Günter R, Felix VS, Roland Z (2010) Microfluidic lab-on-a-chip platforms: requirements, characteristics and applications. Chem Soc Rev 39:1153–1182CrossRefGoogle Scholar
  6. Delgado SM, Kinahan DJ, Sandoval FS, Julius LA, Kilcawley NA, Ducree J, Mager D (2016) Fully automated chemiluminescence detection using an electrified-lab-on-a-disc (eLoaD) platform. Lab Chip 16:4002–4011.  https://doi.org/10.1039/c6lc00973e CrossRefGoogle Scholar
  7. Ducrée J, Haeberle S, Lutz S, Pausch S, Stetten FV, Zengerle R (2007) The centrifugal microfluidic bio-disk platform. J Micromech Microeng 17:S103–S115CrossRefGoogle Scholar
  8. Garcia-Cordero JL, Kurzbuch D, Benito-Lopez F, Diamond D, Lee LP, Ricco AJ (2010) Optically addressable single-use microfluidic valves by laser printer lithography. Lab Chip 10:2680–2687.  https://doi.org/10.1039/c004980h CrossRefGoogle Scholar
  9. Gorkin R et al (2010) Centrifugal microfluidics for biomedical applications. Lab Chip 10:1758–1773.  https://doi.org/10.1039/b924109d CrossRefGoogle Scholar
  10. Gorkin R 3rd et al (2012) Centrifugo-pneumatic valving utilizing dissolvable films. Lab Chip 12:2894–2902.  https://doi.org/10.1039/c2lc20973j CrossRefGoogle Scholar
  11. Haeberle S, Mark D, Stetten FV, Zengerle R (2007) Microfluidic platforms for lab-on-a-chip applications. Lab Chip 7:1094–1110CrossRefGoogle Scholar
  12. Hofflin J, Torres Delgado SM, Suarez Sandoval F, Korvink JG, Mager D (2015) Electrifying the disk: a modular rotating platform for wireless power and data transmission for lab on a disk application. Lab Chip 15:2584–2587.  https://doi.org/10.1039/c5lc00138b CrossRefGoogle Scholar
  13. Kim TH, Park J, Kim CJ, Cho YK (2014) Fully integrated lab-on-a-disc for nucleic acid analysis of food-borne pathogens. Anal Chem 86:3841–3848.  https://doi.org/10.1021/ac403971h CrossRefGoogle Scholar
  14. Kong LX, Perebikovsky A, Moebius J, Kulinsky L, Madou M (2016) Lab-on-a-CD: a fully integrated molecular diagnostic system. J Lab Autom 21:323–355.  https://doi.org/10.1177/2211068215588456 CrossRefGoogle Scholar
  15. Lee BS, Lee JN, Park JM, Lee JG, Kim S, Cho YK, Ko C (2009) A fully automated immunoassay from whole blood on a disc. Lab Chip 9:1548–1555CrossRefGoogle Scholar
  16. Lee BS et al (2011) Fully integrated lab-on-a-disc for simultaneous analysis of biochemistry and immunoassay from whole blood. Lab Chip 11:70–78.  https://doi.org/10.1039/c0lc00205d CrossRefGoogle Scholar
  17. Maguire I, O’Kennedy R, Ducrée J, Regan F (2018) A review of centrifugal microfluidics in environmental monitoring. Anal Methods 10:1497–1515CrossRefGoogle Scholar
  18. Mark D, Metz T, Haeberle S, Lutz S, Ducree J, Zengerle R, von Stetten F (2009) Centrifugo-pneumatic valve for metering of highly wetting liquids on centrifugal microfluidic platforms. Lab Chip 9:3599–3603.  https://doi.org/10.1039/b914415c CrossRefGoogle Scholar
  19. Myung JH, Hong S (2015) Microfluidic devices to enrich and isolate circulating tumor cells. Lab Chip 15:4500–4511.  https://doi.org/10.1039/c5lc00947b CrossRefGoogle Scholar
  20. Park JM, Cho YK, Lee BS, Lee JG, Ko C (2007) Multifunctional microvalves control by optical illumination on nanoheaters and its application in centrifugal microfluidic devices. Lab Chip 7:557–564.  https://doi.org/10.1039/b616112j CrossRefGoogle Scholar
  21. Park J, Sunkara V, Kim TH, Hwang H, Cho YK (2012) Lab-on-a-disc for fully integrated multiplex immunoassays. Anal Chem 84:2133–2140.  https://doi.org/10.1021/ac203163u CrossRefGoogle Scholar
  22. Srinivasan V, Pamula VK, Fair RB (2004) An integrated digital microfluidic lab-on-a-chip for clinical diagnostics on human physiological fluids. Lab Chip 4:310–315CrossRefGoogle Scholar
  23. Strohmeier O et al (2015) Centrifugal microfluidic platforms: advanced unit operations and applications. Chem Soc Rev 44:6187–6229.  https://doi.org/10.1039/c4cs00371c CrossRefGoogle Scholar
  24. Torres Delgado SM et al (2018a) Wirelessly powered and remotely controlled valve-array for highly multiplexed analytical assay automation on a centrifugal microfluidic platform. Biosens Bioelectron 109:214–223.  https://doi.org/10.1016/j.bios.2018.03.012 CrossRefGoogle Scholar
  25. Torres Delgado SM, Korvink JG, Mager D (2018b) The eLoaD platform endows centrifugal microfluidics with on-disc power and communication. Biosens Bioelectron 117:464–473.  https://doi.org/10.1016/j.bios.2018.05.056 CrossRefGoogle Scholar
  26. Wang G et al (2013) A lab-in-a-droplet bioassay strategy for centrifugal microfluidics with density difference pumping, power to disc and bidirectional flow control. Lab Chip 13:3698–3706.  https://doi.org/10.1039/c3lc50545f CrossRefGoogle Scholar
  27. Wang G et al (2018a) Binary centrifugal microfluidics enabling novel, digital addressable functions for valving and routing. Lab Chip.  https://doi.org/10.1039/c8lc00026c CrossRefGoogle Scholar
  28. Wang Y, Wang H, Deng P, Chen W, Guo Y, Tao T, Qin J (2018b) In situ differentiation and generation of functional liver organoids from human iPSCs in a 3D perfusable chip system. Lab Chip 18:3606–3616.  https://doi.org/10.1039/c8lc00869h CrossRefGoogle Scholar
  29. Xi Y, Duford DA, Salin ED (2010) Automated liquid–solid extraction of pyrene from soil on centrifugal microfluidic devices. Talanta 82:1072–1076.  https://doi.org/10.1016/j.talanta.2010.06.007 CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Key Laboratory of Intelligent Optical Sensing and Manipulation of Ministry of EducationNanjing UniversityJiangsuChina
  2. 2.College of Engineering and Applied SciencesNanjing UniversityJiangsuChina
  3. 3.Institute of Optical Communication EngineeringNanjing UniversityJiangsuChina
  4. 4.Department of Biomedical EngineeringThe Chinese University of Hong KongHong KongChina

Personalised recommendations