A successive laminar flow extraction for plant medicine preparation by microfluidic chip
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Liquid–liquid solvent extraction is frequently used in the purification of active ingredients from plants, and repeated extraction by different solvents with different polarity is often demanded to improve purity. According to this demand, two kinds of laminar flow extraction were designed on the microfluidic chip, i.e. a three-phase laminar chip and a successive laminar flow chip, and compared with a current two-phase chip. P. ginseng was used as a model plant to demonstrate extraction performance, and ether and n-butanol were used as the degrease solvent and extraction solvent, respectively. The results demonstrated that the extraction efficiency of a successive laminar flow chip is higher than that of a three-phase laminar flow chip, and that of both chips are higher than that of a two-phase chip. The chip method provided a new way to extract active ingredients from plants, which should be easily automated and integrated with other units to develop a complex system for sample preparation and ingredient detection.
KeywordsLaminar flow Microfluidics Solvent extraction P. ginseng
The research was funded by the National Natural Science Foundation of China (No:81001600).
- Mao ZL, Li XB, Gong WM, Fan W (2008) Optimization of extraction process of Ginsenoside. Lishizhen Med Materia Med Res 19(11):2762–2763Google Scholar
- Plianwong S, Sripattanaporn A, Waewsa-nga K, Buacheen P, Opanasopit P, Ngawhirunpat T, Rojanarata T (2012) Operator care and eco-concerned development of a fast, facile and economical assay for basic nitrogenous drugs based on simplified ion-pair mini-scale extraction using safer solvent combined with drop-based spectrophotometry. Talanta 98:220–225CrossRefGoogle Scholar
- Sun YS, Li W, Wang JH, Bi JJ, Liu ZB, Wang Y, Guo ZD (2010) Optimization of supercritical fluid extraction of saikosaponins from Bupleurum falcatum with orthogonal array design. J Sep Sci 33(8):1161–1166Google Scholar
- Süntar I, Akkol EK, Keles H, Yesilada E, Sarker SD, Baykal T (2012) Comparative evaluation of traditional prescriptions from Cichorium intybus, L. for wound healing: stepwise isolation of an active component by in vivo bioassay and its mode of activity. J Ethnopharmacol 143(1):299–309CrossRefGoogle Scholar
- Xu ZA, Wang TBO, Li CY, Bao LY, Ma QM, Miao YN (2002) Brief introduction to the orthogonal test design. Sci/Tech Inform Dev Econ 12(5):148–150Google Scholar
- Yang LF, Liu HC, Feng FQ, Li DN, Jiang MG (2014) HPLC fingerprint and antitumor activity of different polar parts extracted from crude stems of Derris eriocarpa How. Chin J Pharm Anal 34(12):2112–2118Google Scholar
- Yang MY, Gu YH, Wu XL, Xi XF, Yang XL, Zhou WF, Zeng HZ, Zhang SB, Lu RH, Gao HX, Li J (2018) Rapid analysis of fungicides in tea infusions using ionic liquid immobilized fabric phase sorptive extraction with the assistance of surfactant fungicides analysis using IL-FPSE assisted with surfactant. Food Chem 239:797–805CrossRefGoogle Scholar