Advertisement

A successive laminar flow extraction for plant medicine preparation by microfluidic chip

  • Weiwei Qin
  • Yang He
  • Jinyi Xiao
  • Shengwang Liang
  • Shumei Wang
  • Paul C. H. LiEmail author
  • Yue SunEmail author
Research Paper
  • 83 Downloads

Abstract

Liquid–liquid solvent extraction is frequently used in the purification of active ingredients from plants, and repeated extraction by different solvents with different polarity is often demanded to improve purity. According to this demand, two kinds of laminar flow extraction were designed on the microfluidic chip, i.e. a three-phase laminar chip and a successive laminar flow chip, and compared with a current two-phase chip. P. ginseng was used as a model plant to demonstrate extraction performance, and ether and n-butanol were used as the degrease solvent and extraction solvent, respectively. The results demonstrated that the extraction efficiency of a successive laminar flow chip is higher than that of a three-phase laminar flow chip, and that of both chips are higher than that of a two-phase chip. The chip method provided a new way to extract active ingredients from plants, which should be easily automated and integrated with other units to develop a complex system for sample preparation and ingredient detection.

Keywords

Laminar flow Microfluidics Solvent extraction P. ginseng 

Notes

Acknowledgements

The research was funded by the National Natural Science Foundation of China (No:81001600).

References

  1. Arora A, Simone G, Salieb-Beugelaar GB, Kim JT, Manz A (2010) Latest developments in micro total analysis systems. Anal Chem 82(12):4830–4847CrossRefGoogle Scholar
  2. Beard NP, Edel JB, Demello AJ (2010) Integrated on-chip derivatization and electrophoresis for the rapid analysis of biogenic amines. Electrophoresis 25(14):2363–2373CrossRefGoogle Scholar
  3. Hatch A, Kamholz AE, Hawkins KR, Munson MS, Schilling EA, Weigl BH, Yager P (2001) A rapid diffusion immunoassay in a T-sensor. Nat Biotechnol 19(5):461–465CrossRefGoogle Scholar
  4. Hibara A, Nonaka M, Hisamoto H, Uchiyama K, Kikutani Y, Tokeshi AM, Kitamori T (2003) Stabilization of liquid interface and control of two-phase confluence and separation in glass microchips by utilizing octadecylsilane modification of microchannels. Anal Chem 74(7):1724–1728CrossRefGoogle Scholar
  5. Hisamoto H, Shimizu Y, Uchiyama K, Tokeshi M, Kikutani Y, Hibara A, Kitamori T (2003) Chemicofunctional membrane for integrated chemical processes on a microchip. Anal Chem 75(2):350–354CrossRefGoogle Scholar
  6. Hu YZ, Peng HD, Yan YS, Guan SY, Wang SM, Li PCH, Sun Y (2017) Integration of laminar flow extraction and capillary electrophoretic separation in one microfluidic chip for detection of plant alkaloids in blood samples. Anal Chim Acta 985:121–128CrossRefGoogle Scholar
  7. Huie CW (2002) A review of modern sample-preparation techniques for the extraction and analysis of medicinal plants. Anal Bioanal Chem 373(1–2):23–30CrossRefGoogle Scholar
  8. Mao ZL, Li XB, Gong WM, Fan W (2008) Optimization of extraction process of Ginsenoside. Lishizhen Med Materia Med Res 19(11):2762–2763Google Scholar
  9. Mi JN, Zhang M, Ren GX, Zhang HY, Wang YR, Hu P (2012) Enriched separation of protopanaxatriol ginsenosides, malonyl ginsenosides and protopanaxadiol ginsenosides from Panax ginseng using macroporous resins. J Food Eng 113(4):577–588CrossRefGoogle Scholar
  10. Mu X, Liang QL, Hu P, Ren KN, Wang YM, Luo GA (2010) Selectively modified microfluidic chip for solvent extraction of Radix Salvia miltiorrhiza using three-phase laminar flow to provide double liquid–liquid interface area. Microfluid Nanofluid 9(2–3):365–373CrossRefGoogle Scholar
  11. Plianwong S, Sripattanaporn A, Waewsa-nga K, Buacheen P, Opanasopit P, Ngawhirunpat T, Rojanarata T (2012) Operator care and eco-concerned development of a fast, facile and economical assay for basic nitrogenous drugs based on simplified ion-pair mini-scale extraction using safer solvent combined with drop-based spectrophotometry. Talanta 98:220–225CrossRefGoogle Scholar
  12. Shi W, Wang YT, Li J, Zhang HQ, Ding L (2007) Investigation of ginsenosides in different parts and ages of panax ginseng. Food Chem 102(3):664–668CrossRefGoogle Scholar
  13. Shui LL, Eijkel JCT, van DBA (2007) Multiphase flow in microfluidic systems-control and 8 applications of droplets and interfaces. Adv Colloid Interface Sci 133(1):35–49CrossRefGoogle Scholar
  14. Sun YS, Li W, Wang JH, Bi JJ, Liu ZB, Wang Y, Guo ZD (2010) Optimization of supercritical fluid extraction of saikosaponins from Bupleurum falcatum with orthogonal array design. J Sep Sci 33(8):1161–1166Google Scholar
  15. Sun Y, Yuan JC, Pang JL, Li XN, Wang SM, Zhou YL, Xu F, Li PCH, Jiang SS, Chen H (2018) Millifluidic chip with a modular design used as a sample pretreatment cartridge for flour and flour food products. Talanta 179:719–725CrossRefGoogle Scholar
  16. Süntar I, Akkol EK, Keles H, Yesilada E, Sarker SD, Baykal T (2012) Comparative evaluation of traditional prescriptions from Cichorium intybus, L. for wound healing: stepwise isolation of an active component by in vivo bioassay and its mode of activity. J Ethnopharmacol 143(1):299–309CrossRefGoogle Scholar
  17. Tetala KK, Swarts JW, Chen B, Janssen AE, van Beek TA (2009) A three-phase microfluidic chip for rapid sample clean-up of alkaloids from plant extracts. Lab Chip 9(14):2085–2092CrossRefGoogle Scholar
  18. Toh YC, Zhang C, Zhang J, Khong YM, Chang S, Samper VD, Van ND, Hutmacher DW, Yu H (2007) A novel 3D mammalian cell perfusion-culture system in microfluidic channels. Lab Chip 7(3):302–309CrossRefGoogle Scholar
  19. Tokeshi M, Minagawa T, Uchiyama K, Hibara A, Sato K, Hisamoto H, Kitamori T (2002) Continuous-flow chemical processing on a microchip by combining microunit operations and a multiphase flow network. Anal Chem 74(7):1565–1571CrossRefGoogle Scholar
  20. Vitalini S, Madeo M, Tava A, Iriti M, Vallone L, Avato P, Cocuzza CE, Simonetti P, Argentieri MP (2016) Chemical profile, antioxidant and antibacterial activities of achillea Moschatawulfen, an endemic species from the alps. Molecules 21(7):830CrossRefGoogle Scholar
  21. Xu ZA, Wang TBO, Li CY, Bao LY, Ma QM, Miao YN (2002) Brief introduction to the orthogonal test design. Sci/Tech Inform Dev Econ 12(5):148–150Google Scholar
  22. Yang LF, Liu HC, Feng FQ, Li DN, Jiang MG (2014) HPLC fingerprint and antitumor activity of different polar parts extracted from crude stems of Derris eriocarpa How. Chin J Pharm Anal 34(12):2112–2118Google Scholar
  23. Yang R, Pagaduan JV, Yu M, Woolley AT (2015) On chip preconcentration and fluorescence labeling of model proteins by use of monolithic columns: device fabrication, optimization, and automation. Anal Bioanal Chem 407(3):737–747CrossRefGoogle Scholar
  24. Yang MY, Gu YH, Wu XL, Xi XF, Yang XL, Zhou WF, Zeng HZ, Zhang SB, Lu RH, Gao HX, Li J (2018) Rapid analysis of fungicides in tea infusions using ionic liquid immobilized fabric phase sorptive extraction with the assistance of surfactant fungicides analysis using IL-FPSE assisted with surfactant. Food Chem 239:797–805CrossRefGoogle Scholar
  25. Zhang J, Wei XF, Zeng R, Xu F, Li XJ (2017) Stem cell culture and differentiation in microfluidic devices toward organ-on-a-chip. Future Sci OA 3(2):FSO187CrossRefGoogle Scholar
  26. Zhao B, Viernes NO, Moore JS, Beebe DJ (2002) Control and applications of immiscible liquids in microchannels. J Am Chem Soc 124(19):5284–5285CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.School of Traditional Chinese MedicineGuangdong Pharmaceutical UniversityGuangzhouChina
  2. 2.Key Laboratory of State Administration of TCM for Digital Quality Evaluation of Chinese Materia MedicaGuangzhouChina
  3. 3.Engineering & Technology Research Center for Chinese Materia Medica Quality of Guangdong ProvinceGuangzhouChina
  4. 4.School of Chemistry and Chemical EngineeringGuangdong Pharmaceutical UniversityGuangzhouChina
  5. 5.Department of ChemistrySimon Fraser UniversityBurnabyCanada
  6. 6.Zhengzhou Yellow River Nursing Vocational CollegeZhengzhouChina

Personalised recommendations