Multi-axial non-contact in situ micromanipulation by steady streaming around two oscillating cylinders on holonomic miniature robots Research Paper First Online: 20 July 2018 Abstract
In the field of micromanipulation, an in situ three-axial rotation of a microscale object remains difficult to realize, with rotational resolution and repeatability remaining low. In this paper, we describe the fundamental principle, properties, and experimental results of multi-axial non-contact in situ micromanipulation of an egg cell driven by steady streaming generated around an oscillating cylinder. A continuously oscillating cylinder generates the steady streaming that draws an egg cell toward the cylinder. If it is trapped by an eddy near the tip of the cylinder, it continuously rotates around the vertical axis at a fixed point. If it is trapped by a swirl flow generated around the side of the cylinder, it rotates around the horizontal axis. We define Reynolds number,
R e, as ar c ω/ ν, where a is half of the oscillation’s amplitude, r c is the cylinder’s radius, ω is the oscillation’s angular frequency, and ν is the kinematic viscosity. We demonstrate that the conditions of the vertical and horizontal rotations are determined by two dimensionless numbers: R e and a/ r c. In our experiments, we obtained rotational resolutions of 0.05° and 0.11° and maximal angular velocities of 34.8°/s and 188°/s for the vertical and horizontal rotations, respectively. We also developed unique micromanipulation methods using two oscillating pipettes attached to holonomic miniature robots. We successfully manipulated five degrees of freedom (DoF) of the cell (three posture angles and two translational displacements along the X and Y axes) with the steady streaming. The proposed method enables a multi-axial, non-contact, in situ, and compact micromanipulation independent of the electrical, optical, magnetic, shape, and stiffness properties of the objects; moreover, it can be applied in microfluidics, biomedical, and heterogeneous microassembly applications. Keywords In situ multi-axial micromanipulation Steady streaming Oscillating cylinder Holonomic miniature robot Electronic supplementary material
The online version of this article (
) contains supplementary material, which is available to authorized users. https://doi.org/10.1007/s10404-018-2098-5 Notes Acknowledgements
We wish to thank Mr. Naoto Chiba for providing a significant amount of experimental data over 3 years. We also thank Dr. Shizue Iwasaki for the support and for providing the experimental data related to the processing of egg cells, which aided us to apply our technology to biomedical problems. We also thank Dr. Binghu Piao for computing the alternating torque and forces exerted on the cell by an oscillating cylinder, which aided us to understand the elusive microfluidics phenomena. This work was partially supported by the Industrial Technology Research Grant Program (Grant for Young Researchers) of New Energy and Industrial Technology Development Organization (NEDO), Japan (2004–2006).
Compliance with ethical standards Conflict of interest
The authors declare that they have no conflict of interest.
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