Multi-axial non-contact in situ micromanipulation by steady streaming around two oscillating cylinders on holonomic miniature robots Research Paper First Online: 20 July 2018 Received: 17 April 2018 Accepted: 09 July 2018 Abstract
In the field of micromanipulation, an in situ three-axial rotation of a microscale object remains difficult to realize, with rotational resolution and repeatability remaining low. In this paper, we describe the fundamental principle, properties, and experimental results of multi-axial non-contact in situ micromanipulation of an egg cell driven by steady streaming generated around an oscillating cylinder. A continuously oscillating cylinder generates the steady streaming that draws an egg cell toward the cylinder. If it is trapped by an eddy near the tip of the cylinder, it continuously rotates around the vertical axis at a fixed point. If it is trapped by a swirl flow generated around the side of the cylinder, it rotates around the horizontal axis. We define Reynolds number,
R e, as ar c ω/ ν, where a is half of the oscillation’s amplitude, r c is the cylinder’s radius, ω is the oscillation’s angular frequency, and ν is the kinematic viscosity. We demonstrate that the conditions of the vertical and horizontal rotations are determined by two dimensionless numbers: R e and a/ r c. In our experiments, we obtained rotational resolutions of 0.05° and 0.11° and maximal angular velocities of 34.8°/s and 188°/s for the vertical and horizontal rotations, respectively. We also developed unique micromanipulation methods using two oscillating pipettes attached to holonomic miniature robots. We successfully manipulated five degrees of freedom (DoF) of the cell (three posture angles and two translational displacements along the X and Y axes) with the steady streaming. The proposed method enables a multi-axial, non-contact, in situ, and compact micromanipulation independent of the electrical, optical, magnetic, shape, and stiffness properties of the objects; moreover, it can be applied in microfluidics, biomedical, and heterogeneous microassembly applications. Keywords In situ multi-axial micromanipulation Steady streaming Oscillating cylinder Holonomic miniature robot Electronic supplementary material
The online version of this article (
) contains supplementary material, which is available to authorized users. https://doi.org/10.1007/s10404-018-2098-5 Notes Acknowledgements
We wish to thank Mr. Naoto Chiba for providing a significant amount of experimental data over 3 years. We also thank Dr. Shizue Iwasaki for the support and for providing the experimental data related to the processing of egg cells, which aided us to apply our technology to biomedical problems. We also thank Dr. Binghu Piao for computing the alternating torque and forces exerted on the cell by an oscillating cylinder, which aided us to understand the elusive microfluidics phenomena. This work was partially supported by the Industrial Technology Research Grant Program (Grant for Young Researchers) of New Energy and Industrial Technology Development Organization (NEDO), Japan (2004–2006).
Compliance with ethical standards Conflict of interest
The authors declare that they have no conflict of interest.
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Amit R, Abadi A, Kosa G (2016) Characterization of steady streaming for a particle manipulation system. Biomed Microdevices 18:39.
https://doi.org/10.1007/s10544-016-0055-1 CrossRef Google Scholar
Aoyama H, Fuchiwaki O (2001) Flexible micro-processing by multiple micro robots in SEM. Proc ICRA:3429–3434.
Aoyama H, Chiba N, Fuchiwaki O et al (2006) Non-contact bio cell manipulation by nonlinear micro flow around the vibrated pipette on micro robot. In: Proc of ASPE Annual Meeting, Poster sessions mechatronics-1
Benhal P, Chase JG, Gaynor P et al (2014) AC Electric field induced dipole-based on-chip 3D cell rotation. Lab Chip 14(15):2717–2727.
https://doi.org/10.1039/c4lc00312h CrossRef Google Scholar
Bianchi V et al (2007) Differential sucrose concentration during dehydration (0.2 mol/l) and rehydration (0.3 mol/l) increases the implantation rate of frozen human oocytes. RBM Online 14(1):64–71
MathSciNet Google Scholar
Borini A et al (2006) Clinical outcome of oocyte cryopreservation after slow cooling with a protocol utilizing a high sucrose concentration. Hum Reprod 21(2):512–517.
https://doi.org/10.1093/humrep/dei346 CrossRef Google Scholar
Boudaoud M, Haddab Y, Gorrec YL (2012) Modeling and optimal force control of a nonlinear electrostatic microgripper. TMECH 18(3):1130–1139.
https://doi.org/10.1109/TMECH.2012.2197216 Google Scholar
Büyükkoçak S, Özer MB, Çetin B (2014) Numerical modeling of ultrasonic particle manipulation for microfluidic applications. Microfluid Nanofluid 17(6):1025–1037.
https://doi.org/10.1007/s10404-014-1398-7 CrossRef Google Scholar
Carroll J et al (1990) Freeze-thaw-induced changes of the zona pellucida explains decreased rates of fertilization in frozen-thawed mouse oocytes. J Reprod Fertil 90:547–553
CrossRef Google Scholar
Cecil J, Kumar MBBR, Lu Y et al (2016) A review of micro-devices assembly techniques and technology. Int J Adv Manuf Tech 83(9):1569–1581.
https://doi.org/10.1007/s00170-015-7698-6 CrossRef Google Scholar
Chen T, Sun L, Chen L et al (2010) A hybrid-type electrostatically driven microgripper with an integrated vacuum tool. Sensor Actuatators A-Phys 158(2):320–327.
https://doi.org/10.1016/j.sna.2010.01.001 CrossRef Google Scholar
Chen H, Wang C, Sun D (2013) Dynamics calibration of optically trapped cells with adaptive control technology. Proc ICRA.
Chowdhury S, Thakur A, Wang C et al (2013) Automated indirect manipulation of irregular shaped cells with optical tweezers for studying collective cell migration. Proc ICRA.
Coenen W (2016) Steady streaming around a cylinder pair. Proc R Soc A 472(2195):20160522.
https://doi.org/10.1098/rspa.2016.0522 MathSciNet CrossRef zbMATH Google Scholar
Destgeera G, Sung HJ (2015) Recent advances in microfluidic actuation and micro-object manipulation via surface acoustic waves. Lab Chip 15:2722–2738.
https://doi.org/10.1039/c5lc00265f CrossRef Google Scholar
Elbuken C, Khamesee MB, Yavuz M (2009) Design and implementation of a micromanipulation system using a magnetically levitated. MEMS Robot TMECH 14:4.
https://doi.org/10.1109/TMECH.2009.2023648 Google Scholar
Fan Z, Wang L, Rong W, Sun L (2015) Dropwise condensation on a hydrophobic probe-tip for manipulating micro-objects. Appl Phys Lett 106:084105.
https://doi.org/10.1063/1.4913849 CrossRef Google Scholar
Fatikow S, Seyfried J, Fahlbusch S et al (2000) A flexible microrobot-based microassembly station. J Intell Robot Syst 27(1):135–169.
https://doi.org/10.1023/A:1008106331459 CrossRef zbMATH Google Scholar
Floyd S, Pawashe C, Sitti (2009) Two-dimensional contact and noncontact micromanipulation in liquid using an untethered mobile magnetic microrobot. M TRO 25(6):1332–1342.
https://doi.org/10.1109/TRO.2009.2028761 Google Scholar
Friend J, Yeo L (2011) Microscale acoustofluidics: microfluidics driven via acoustics and ultrasonics. Rev Mod Phys 83(2):647–687.
https://doi.org/10.1103/RevModPhys.83.647 CrossRef Google Scholar
Fuchiwaki O (2013) Insect-sized holonomic robots for precise, omnidirectional, and flexible microscopic processing: identification, design, development, and basic experiments. PE 37(1):88–106.
https://doi.org/10.1016/j.precisioneng.2012.07.004 Google Scholar
Fuchiwaki O, Kumagai K (2013) Development of wet tweezers based on capillary force for complex-shaped and heterogeneous micro-assembly. Proc IROS.
Fuchiwaki O, Ito A, Misaki D, Aoyama H (2008) Multi-axial micromanipulation organized by versatile micro robots and micro tweezers. Proc ICRA.
Fuchiwaki O, Chiba N, Aoyama H (2010) U.S. Patent 2010 US 7726210
Fuchiwaki O, Arafuka K, Omura S (2012) Development of 3-DOF inchworm mechanism for flexible, compact, low-inertia, and omnidirectional precise positioning: dynamical analysis and improvement of the maximum velocity within no slip of electromagnets. TMECH 17(4):697–708.
https://doi.org/10.1109/TMECH.2011.2118764 Google Scholar
Fuchiwaki O, Yatsurugi M, Sato T (2014) The basic performance of a miniature omnidirectional 6-legged inchworm robot from cm- to µm-scale precise positioning. Trans Mat Res Soc Japan 39(2):211–215.
https://doi.org/10.14723/tmrsj.39.211 CrossRef Google Scholar
Fuchiwaki O, Yamagiwa T, Omura S, Hara Y (2015) In-situ repetitive calibration of microscopic probes maneuvered by holonomic inchworm robot for flexible microscopic operations. Proc IROS.
Gijs MAM (2004) Magnetic bead handling on-chip: new opportunities for analytical applications. Microfluid Nanofluid 1(1):22–40.
https://doi.org/10.1007/s10404-004-0010-y Google Scholar
Grier GD (2003) A revolution in optical manipulation. Nature 424:810–816.
https://doi.org/10.1038/nature01935 CrossRef Google Scholar
Hatamura Y, Nakao M, Sato T (1995) Construction of nano manufacturing world. Microsyst Technol 1:155–162
CrossRef Google Scholar
Hattori T, Kamiyama K, Kojima M et al (2015) Generation of swirl flow by needle vibration for micro manipulation. Proc IROS.
Hayakawa T, Sakuma S, Fukuhara T et al (2014) A single cell extraction chip using vibration-induced
Hayakawa T et al (2015) On-chip cell transportation based on vibration-induced local flow in open chip environment. Proc IROS.
Hyakutake T, Nagai S (2015) Numerical simulation of red blood cell distributions in three-dimensional microvascular bifurcations. Microvasc Res 97:115–123.
https://doi.org/10.1016/j.mvr.2014.10.001 CrossRef Google Scholar
Hyakutake T, Suzuki H, Yamamoto S (2015) Effect ofnon-Newtonian fluid properties on bovine sperm motility. J Biomech 48:2941–2947.
https://doi.org/10.1016/j.jbiomech.2015.08.005 CrossRef Google Scholar
Jeong JS, Lee JW, Lee CY et al (2011) Particle manipulation in a microfluidic channel using acoustic trap. Biomed Microdevices 13(4):779–788.
https://doi.org/10.1007/s10544-011-9548-0 CrossRef Google Scholar
Kim K, Liu X, Zhang Y, Sun Y (2008) Nanonewton force-controlled manipulation of biological cells using a monolithic MEMS microgripper with two-axis force feedback. J Micromech Microeng 18(5):055013.
https://doi.org/10.1088/0960-1317/18/5/055013 CrossRef Google Scholar
Kumagai K, Fuchiwaki O (2012) A development of dispenser for high-viscosity liquid and pick and place of micro objects using capillary force. KEM 516:48–53.
https://doi.org/10.4028/www.scientific.net/KEM.516.48 CrossRef Google Scholar
Leibacher I, Hahn P, Dual J (2015) Acoustophoretic cell and particle trapping on microfluidic sharp edges. Microfluid Nanofluid 19(4):923–933.
https://doi.org/10.1007/s10404-015-1621-1 CrossRef Google Scholar
Leung C, Lu Z, Zhang XP et al (2012) Three-dimensional rotation of mouse embryos. IEEE Trans Biomed Eng 59(4):1049–1056
https://doi.org/10.1109/TBME.2012.2182995 CrossRef Google Scholar
Lieu VH, House TA, Schwartz DT et al (2012) Hydrodynamic tweezers: impact of design geometry on flow and microparticle trapping. Anal Chem 84:1963–1968.
https://doi.org/10.1021/ac203002z CrossRef Google Scholar
Misaki D, Naoto C, Takeshi U, Fuchiwaki O et al (2004) 3D micro image recognition for in bio-cell operation by micro robots. In: Proceedings of ASPE Annual Meeting, pp 273–276
Misaki D, Oguri A, Zhu N, Fuchiwaki O et al (2007) 3D model reconstruction with non-contact rotation mechanism using micro robots. In: Proceedings of ASPE Annual Meeting, pp 260–263
Nakagata N (1989) High survival rate of unfertilized mouse oocytes after vitrification. J Reprod Fertil 87:479–483
CrossRef Google Scholar
Nuriev A, Zaitseva O (2014) Analysis of the secondary stationary flow around an oscillating circular cylinder. Int J Mech Aero Ind Mechatron Manuf Eng 8:11.
https://doi.org/10.1999/1307-6892/9999758 Google Scholar
Obata KJ, Motokado T, Saito S et al (2004) A scheme for micro-manipulation based on capillary force. J Fluid Mech 498:113–121.
https://doi.org/10.1017/S0022112003006955 MathSciNet CrossRef zbMATH Google Scholar
Ozkan M, Wang M, Ozkan C et al (2003) Optical manipulation of objects and biological cells in microfluidic devices. Biomed Microdevices 5:61–67.
https://doi.org/10.1023/A:1024467417471 CrossRef Google Scholar
Rong W, Fan Z, Wang L et al (2014) A vacuum microgripping tool with integrated vibration releasing capability. Rev Sci Instrum 85:085002.
https://doi.org/10.1063/1.4891695 CrossRef Google Scholar
Sadhal SS (2012) Acoustofluidics 13: analysis of acoustic streaming by perturbation methods. Lab Chip 12:2292–2300.
https://doi.org/10.1039/c2lc40202e CrossRef Google Scholar
Savia M, Koivo HN (2009) Contact micromanipulation—survey of strategies. TMECH 14(4):504–514.
https://doi.org/10.1109/TMECH.2008.2011986 Google Scholar
Shafiee H, Caldwell JL, Sano MB et al (2009) Contactless dielectrophoresis: a new technique for cell manipulation. Biomed Microdevices 11:997–1006.
https://doi.org/10.1007/s10544-009-9317-5 CrossRef Google Scholar
Tamadazte B, Piat NL, Dembele S (2011) Robotic micromanipulation and microassembly using monoview and multiscale visual servoing. TMECH 16(2):277–287.
https://doi.org/10.1109/TMECH.2010.2040900 Google Scholar
Tatsuno M (1973) Circulatory streaming around an oscillating circular cylinder at low reynolds numbers. J Phys Soc Jpn 35:915–920
CrossRef Google Scholar
Tatsuno M (1981) Secondary flow induced by a circular cylinder performing unharmonic oscillation. J Phys Soc Jpn 50:330–337
CrossRef Google Scholar
Walker R, Gralinski I, Lay KK et al (2012) Particle manipulation using an ultrasonic micro-gripper. Appl Phys Lett 101:163504.
https://doi.org/10.1063/1.4759127 CrossRef Google Scholar
Wang CY, Drachman B (1982) The steady streaming generated by a vibrating plate parallel to a fixed plate. Appl Sci Res 39(1):55–68
CrossRef zbMATH Google Scholar
Whirling Flow and a Thermo-Responsive Gel Pattern. Micromachines 5(3):681–696.
Wiklund M, Green R, Ohlin M (2012) Acoustofluidics 14: applications of acoustic streaming in microfluidic devices. Lab Chip 12:2438–2451.
https://doi.org/10.1039/c2lc40203c CrossRef Google Scholar
Xie H, Regnier S (2011) Development of a flexible robotic system for multiscale applications of micro/nanoscale manipulation and assembly. TMECH 16(2):266–276.
https://doi.org/10.1109/TMECH.2010.2040483 Google Scholar
Yabugaki H, Ohara K, Kojima M et al (2013) Automated stable grasping with two-fingered microhand using micro force sensor. Proc ICRA.
Yalikun Y, Kanda Y, Morishima K (2016a) Hydrodynamic vertical rotation method for a single cell in an open space. Microfluid Nanofluid 20(74)
Yalikun Y, Kanda Y, Morishima K (2016b) A method of three-dimensional micro-rotational flow generation for biological applications. Micromachines 7(8):140.
https://doi.org/10.3390/mi7080140 CrossRef Google Scholar
Ye Z, Diller E, Sitti M (2012) Micro-manipulation using rotational fluid flows induced by remote magnetic micromanipulators. J Appl Phys 112(064912).
Yesin KB, Vollmers K, Nelson BJ (2006) Modeling and control of untethered biomicrorobots in a fluidic environment using electromagnetic fields. Robot Res 25(5–6):527–536.
https://doi.org/10.1177/0278364906065389 CrossRef Google Scholar
Zhang Y, Chen BK, Liu X, Sun Y (2010) autonomous robotic pick-and-place of microobjects. TRO 26(1):200–207.
https://doi.org/10.1109/TRO.2009.2034831 Google Scholar Copyright information
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