Single-cell compressibility quantification for assessing metastatic potential of cancer cells through multi-frequency acoustophoresis
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Analyzing cancer cell compressibility with single-cell resolution is of high interest in understanding cancer metastasis as well as in other cell biology applications. Here, a multi-frequency acoustophoretic cell alignment technique to precisely control cell positions in 3D space was developed, and combined with our recently developed numerical acoustophoresis model, which allowed extracting the compressibility of cells with high accuracy. This technology was applied to measure the compressibility of different head and neck cancer (HNC) cell lines that have different metastatic potential. This method can be a simple, non-contact, accurate, and low-cost solution for studying cell biomechanics and utilizing such biomechanical properties in evaluating the metastatic potential of cancer cells.
KeywordsCell compressibility Multi-frequency acoustophoresis Metastatic potential of cancer cells Acoustofluidics
This work was supported by the National Science Foundation (NSF) Grant No. ECCS 1232251.
- Augustsson P, Barnkob R, Grenvall C, Deierborg T, Brundin P, Bruus H, Laurell T (2010) Measuring the acoustophoretic contrast factor of living cells in microchannels. In: Proceedings of the 14 International Conference on Miniaturized Systems for Chemistry and Life Sciences, pp 1337–1339Google Scholar
- Barnkob R, Augustsson P, Magnusson C, Lilja H, Laurell T, Bruus H (2011) Measuring density and compressibility of white blood cells and prostate cancer cells by microchannel acoustophoresis. In: Proceedings of the 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences, pp 127–129Google Scholar
- Cross SE, Jin Y-S, Rao J, Gimzewski JK (2007) Nanomechanical analysis of cells from cancer patients. Nat Nano 2:780–783. http://www.nature.com/nnano/journal/v2/n12/suppinfo/nnano.2007.388_S1.html
- Gorkov LP (1962) On the forces acting on a small particle in an acoustical field in an ideal fluid. Sov Phys Dokl 6:773Google Scholar
- Hur SC, Henderson-MacLennan NK, McCabe ERB, Di Carlo D (2011) Deformability-based cell classification and enrichment using inertial microfluidics. LabChip 11:912–920Google Scholar
- Kim W, Han AA (2010) Micro-aspirator chip using vacuum expanded microchannels for high-throughput mechanical characterization of biological cells. In: The 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences, Groningen, The Netherlands, pp 253–255Google Scholar
- Weigelt B, Peterse JL, van’t Veer LJ (2005) Breast cancer metastasis: markers and models Nat Rev Cancer 5:591–602. http://www.nature.com/nrc/journal/v5/n8/suppinfo/nrc1670_S1.html