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Use microfluidic chips to study the effects of ultraviolet lights on human fibroblasts

  • Chien-Hsun Huang
  • Hsien-San Hou
  • Kai-Yin Lo
  • Ji-Yen Cheng
  • Yung-Shin Sun
Research Paper

Abstract

The ultraviolet (UV) radiation from sunlight exposure is highly related to skin damages such as photoaging and skin cancers. UVA radiation can cause altered expression of extracellular matrix proteins, and UVB is believed to be an apoptosis induction factor. In the past, many experiments have been conducted in vitro to investigate how UV lights are responsible for cell damages. However, most of these studies were done under static conditions such as culture dishes, which were quite different from the in vivo dynamic circulatory system. Also, in these studies, only one UV dose was applied at one time, limiting the experimental throughput. To best mimic the physiological condition as well as to increase the throughput, a microfluidic chip was designed and fabricated to create five different UV doses in one single experimental run. This biocompatible chip is used for seeding and culturing cells, together with observing cell morphology in a circulating condition. By using the “Christmas tree” structure, five different concentrations of blue dye solutions were generated within a microfluidic chip. And by illuminating this chip with the UV light, five different UV doses could be attained on cells cultured within this chip. The effects of UVB dose on NIH/3T3 fibroblasts, mainly in terms of morphology and fluorescence-based cell damage rate, were studied.

Keywords

Microfluidic chips Ultraviolet light NIH/3T3 cells Cell morphology Cell damage 

Notes

Acknowledgements

This work was financially supported by the Ministry of Science and Technology of Taiwan under Contract No. MOST 105-2112-M-030-002-MY2. The authors would like to thank Dr. Huai-Fang Chang for help in cell culturing.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2017

Authors and Affiliations

  1. 1.Department of PhysicsFu-Jen Catholic UniversityNew Taipei CityTaiwan, ROC
  2. 2.Research Center for Applied SciencesAcademia SinicaTaipei CityTaiwan
  3. 3.Department of Agricultural ChemistryNational Taiwan UniversityTaipei CityTaiwan

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