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Flow rate-insensitive microparticle separation and filtration using a microchannel with arc-shaped groove arrays

  • Qianbin Zhao
  • Dan Yuan
  • Sheng Yan
  • Jun Zhang
  • Haiping Du
  • Gursel Alici
  • Weihua Li
Research Paper
Part of the following topical collections:
  1. 2016 International Conference of Microfluidics, Nanofluidics and Lab-on-a-Chip, Dalian, China

Abstract

Inertial microfluidics can separate microparticles in a continuous and high-throughput manner, and is very promising for a wide range of industrial, biomedical and clinical applications. However, most of the proposed inertial microfluidic devices only work effectively at a limited and narrow flow rate range because the performance of inertial particle focusing and separation is normally very sensitive to the flow rate (Reynolds number). In this work, an innovative particle separation method is proposed and developed by taking advantage of the secondary flow and particle inertial lift force in a straight channel (AR = 0.2) with arc-shaped groove arrays patterned on the channel top surface. Through the simulation results achieved, it can be found that a secondary flow is induced within the cross section of the microchannel and guides different-size particles to the corresponding equilibrium positions. On the other hand, the effects of the particle size, flow rate and particle concentration on particle focusing and separation quality were experimentally investigated. In the experiments, the performance of particle focusing, however, was found relatively insensitive to the variation of flow rate. According to this, a separation of 4.8 and 13 µm particle suspensions was designed and successfully achieved in the proposed microchannel, and the results show that a qualified particle separation can be achieved at a wide range of flow rate. This flow rate-insensitive microfluidic separation (filtration) method is able to potentially serve as a reliable biosample preparation processing step for downstream bioassays.

Keywords

Equilibrium Position Particle Suspension Secondary Flow Straight Channel Particle Separation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

This work is partially supported by UOW-CSC Scholarship.

References

  1. Adams AA et al (2008) Highly efficient circulating tumor cell isolation from whole blood and label-free enumeration using polymer-based microfluidics with an integrated conductivity sensor. J Am Chem Soc 130:8633–8641CrossRefGoogle Scholar
  2. Amini H, Lee W, Di Carlo D (2014) Inertial microfluidic physics. Lab Chip 14:2739–2761CrossRefGoogle Scholar
  3. Asmolov ES (1999) The inertial lift on a spherical particle in a plane Poiseuille flow at large channel Reynolds number. J Fluid Mech 381:63–87CrossRefzbMATHGoogle Scholar
  4. Bhagat AAS, Kuntaegowdanahalli SS, Papautsky I (2008) Continuous particle separation in spiral microchannels using dean flows and differential migration. Lab Chip 8:1906–1914CrossRefGoogle Scholar
  5. Bhagat AAS, Bow H, Hou HW, Tan SJ, Han J, Lim CT (2010) Microfluidics for cell separation. Med Biol Eng Comput 48:999–1014CrossRefGoogle Scholar
  6. Çetin B, Li D (2011) Dielectrophoresis in microfluidics technology. Electrophoresis 32:2410–2427CrossRefGoogle Scholar
  7. Choi S, Song S, Choi C, Park J-K (2007) Continuous blood cell separation by hydrophoretic filtration. Lab Chip 7:1532–1538CrossRefGoogle Scholar
  8. Choi S, Song S, Choi C, Park JK (2008) Sheathless focusing of microbeads and blood cells based on hydrophoresis. Small 4:634–641CrossRefGoogle Scholar
  9. Choi YS, Seo KW, Lee SJ (2011) Lateral and cross-lateral focusing of spherical particles in a square microchannel. Lab Chip 11:460–465CrossRefGoogle Scholar
  10. Chung AJ, Gossett DR, Di Carlo D (2013) Three dimensional, sheathless, and high-throughput microparticle inertial focusing through geometry-induced secondary flows. Small 9:685–690CrossRefGoogle Scholar
  11. Di Carlo D (2009) Inertial microfluidics. Lab Chip 9:3038–3046CrossRefGoogle Scholar
  12. Gerlach T (1998) Microdiffusers as dynamic passive valves for micropump applications. Sens Actuators A 69:181–191CrossRefGoogle Scholar
  13. Hoshino K, Huang Y-Y, Lane N, Huebschman M, Uhr JW, Frenkel EP, Zhang X (2011) Microchip-based immunomagnetic detection of circulating tumor cells. Lab Chip 11:3449–3457CrossRefGoogle Scholar
  14. Huang LR, Cox EC, Austin RH, Sturm JC (2004) Continuous particle separation through deterministic lateral displacement. Science 304:987–990CrossRefGoogle Scholar
  15. Hur SC, Brinckerhoff TZ, Walthers CM, Dunn JC, Di Carlo D (2012) Label-free enrichment of adrenal cortical progenitor cells using inertial microfluidics. PLoS ONE 7:e46550CrossRefGoogle Scholar
  16. Jin C, McFaul SM, Duffy SP, Deng X, Tavassoli P, Black PC, Ma H (2014) Technologies for label-free separation of circulating tumor cells: from historical foundations to recent developments. Lab Chip 14:32–44CrossRefGoogle Scholar
  17. Jin T, Yan S, Zhang J, Yuan D, Huang X-F, Li W (2016) A label-free and high-throughput separation of neuron and glial cells using an inertial microfluidic platform. Biomicrofluidics 10:034104CrossRefGoogle Scholar
  18. Kuntaegowdanahalli SS, Bhagat AAS, Kumar G, Papautsky I (2009) Inertial microfluidics for continuous particle separation in spiral microchannels. Lab Chip 9:2973–2980CrossRefGoogle Scholar
  19. Lee MG, Choi S, Kim H-J, Lim HK, Kim J-H, Huh N, Park J-K (2011a) Inertial blood plasma separation in a contraction–expansion array microchannel. Appl Phys Lett 98:253702CrossRefGoogle Scholar
  20. Lee MG, Choi S, Park J-K (2011b) Inertial separation in a contraction–expansion array microchannel. J Chromatogr A 1218:4138–4143CrossRefGoogle Scholar
  21. Lee MG, Shin JH, Bae CY, Choi S, Park J-K (2013) Label-free cancer cell separation from human whole blood using inertial microfluidics at low shear stress. Anal Chem 85:6213–6218CrossRefGoogle Scholar
  22. Li M, Li S, Cao W, Li W, Wen W, Alici G (2012) Continuous particle focusing in a waved microchannel using negative dc dielectrophoresis. J Micromech and Microeng 22:095001CrossRefGoogle Scholar
  23. Liu C, Stakenborg T, Peeters S, Lagae L (2009) Cell manipulation with magnetic particles toward microfluidic cytometry. J Appl Phys 105:102014CrossRefGoogle Scholar
  24. Loutherback K, D’Silva J, Liu L, Wu A, Austin RH, Sturm JC (2012) Deterministic separation of cancer cells from blood at 10 mL/min. AIP Adv 2:042107CrossRefGoogle Scholar
  25. Martel JM, Toner M (2012) Inertial focusing dynamics in spiral microchannels. Phys Fluids (1994-present) 24:032001CrossRefGoogle Scholar
  26. McGrath J, Jimenez M, Bridle H (2014) Deterministic lateral displacement for particle separation: a review. Lab Chip 14:4139–4158CrossRefGoogle Scholar
  27. Nagrath S et al (2007) Isolation of rare circulating tumour cells in cancer patients by microchip technology. Nature 450:1235–1239CrossRefGoogle Scholar
  28. Segre G (1961) Radial particle displacements in Poiseuille flow of suspensions. Nature 189:209–210CrossRefGoogle Scholar
  29. Segre G, Silberberg A (1962) Behaviour of macroscopic rigid spheres in Poiseuille flow Part 2. Experimental results and interpretation. J Fluid Mech 14:136–157CrossRefzbMATHGoogle Scholar
  30. Shi J, Mao X, Ahmed D, Colletti A, Huang TJ (2008) Focusing microparticles in a microfluidic channel with standing surface acoustic waves (SSAW). Lab Chip 8:221–223CrossRefGoogle Scholar
  31. Wang MM et al (2005) Microfluidic sorting of mammalian cells by optical force switching. Nat Biotechnol 23:83–87CrossRefGoogle Scholar
  32. Xie Y, Zheng D, Li Q, Chen Y, Lei H, Pu LL (2010) The effect of centrifugation on viability of fat grafts: an evaluation with the glucose transport test. J Plast Reconstr Aesthet Surg 63:482–487CrossRefGoogle Scholar
  33. Yamada M, Nakashima M, Seki M (2004) Pinched flow fractionation: continuous size separation of particles utilizing a laminar flow profile in a pinched microchannel. Anal Chem 76:5465–5471CrossRefGoogle Scholar
  34. Yan S, Zhang J, Alici G, Du H, Zhu Y, Li W (2014) Isolating plasma from blood using a dielectrophoresis-active hydrophoretic device. Lab Chip 14:2993–3003CrossRefGoogle Scholar
  35. Zhang J, Yan S, Li W, Alici G, Nguyen N-T (2014a) High throughput extraction of plasma using a secondary flow-aided inertial microfluidic device. RSC Adv 4:33149–33159CrossRefGoogle Scholar
  36. Zhang J, Yan S, Sluyter R, Li W, Alici G, Nguyen N-T (2014b) Inertial particle separation by differential equilibrium positions in a symmetrical serpentine micro-channel. Sci Rep 4:4527Google Scholar
  37. Zhang J, Yan S, Yuan D, Alici G, Nguyen N-T, Warkiani ME, Li W (2016) Fundamentals and applications of inertial microfluidics: a review. Lab Chip 16:10–34CrossRefGoogle Scholar
  38. Zhou J, Giridhar PV, Kasper S, Papautsky I (2013) Modulation of aspect ratio for complete separation in an inertial microfluidic channel. Lab Chip 13:1919–1929CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2017

Authors and Affiliations

  1. 1.School of Mechanical, Materials and Mechatronic EngineeringUniversity of WollongongWollongongAustralia
  2. 2.School of Mechanical EngineeringNanjing University of Science and TechnologyNanjingChina
  3. 3.School of Electric, Computer and Telecommunication EngineeringUniversity of WollongongWollongongAustralia

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