Microfluidics and Nanofluidics

, Volume 19, Issue 4, pp 883–890 | Cite as

Deformation of a single mouse oocyte in a constricted microfluidic channel

  • ZhengYuan Luo
  • Sinan Güven
  • Irep Gozen
  • Pu Chen
  • Savas Tasoglu
  • Raymond M. Anchan
  • BoFeng Bai
  • Utkan Demirci
Research Paper

Abstract

Single oocyte manipulation in microfluidic channels via precisely controlled flow is critical in microfluidics-based in vitro fertilization. Such systems can potentially minimize the number of transfer steps among containers for rinsing as often performed during conventional in vitro fertilization and can standardize protocols by minimizing manual handling steps. To study shape deformation of oocytes under shear flow and its subsequent impact on their spindle structure is essential for designing microfluidics for in vitro fertilization. Here, we developed a simple yet powerful approach to (1) trap a single oocyte and induce its deformation through a constricted microfluidic channel, (2) quantify oocyte deformation in real time using a conventional microscope and (3) retrieve the oocyte from the microfluidic device to evaluate changes in their spindle structures. We found that oocytes can be significantly deformed under high flow rates, e.g., 10 μL/min in a constricted channel with a width and height of 50 and 150 μm, respectively. Oocyte spindles can be severely damaged, as shown here by immunocytochemistry staining of the microtubules and chromosomes. The present approach can be useful to investigate underlying mechanisms of oocyte deformation exposed to well-controlled shear stresses in microfluidic channels, which enables a broad range of applications for reproductive medicine.

Keywords

Oocyte deformation Spindle damage Single cell trapping Microfluidics 

Supplementary material

10404_2015_1614_MOESM1_ESM.doc (218 kb)
Supplementary material 1 (DOC 218 kb)

Supplementary material 2 (AVI 9398 kb)

Supplementary material 3 (AVI 18789 kb)

Supplementary material 4 (AVI 10837 kb)

Supplementary material 5 (AVI 10003 kb)

Supplementary material 6 (AVI 2470 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • ZhengYuan Luo
    • 1
    • 2
  • Sinan Güven
    • 1
    • 4
    • 6
  • Irep Gozen
    • 1
  • Pu Chen
    • 1
    • 4
  • Savas Tasoglu
    • 1
    • 5
  • Raymond M. Anchan
    • 3
  • BoFeng Bai
    • 2
  • Utkan Demirci
    • 1
    • 4
  1. 1.Division of Biomedical Engineering, Department of Medicine, Brigham and Women’s HospitalHarvard Medical SchoolBostonUSA
  2. 2.State Key Laboratory of Multiphase Flow in Power EngineeringXi’an Jiaotong UniversityXi’anPeople’s Republic of China
  3. 3.Center for Infertility and Reproductive Surgery, Obstetrics Gynecology and Reproductive Biology, Brigham and Women’s HospitalHarvard Medical SchoolBostonUSA
  4. 4.Demirci Bio-Acoustic MEMS in Medicine (BAMM) Lab, Department of Radiology, Canary Center for Early Cancer DetectionStanford University School of MedicineStanfordUSA
  5. 5.Department of Mechanical EngineeringUniversity of ConnecticutStorrsUSA
  6. 6.Izmir Biomedicine and Genome CenterDokuz Eylul UniversityIzmirTurkey

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