Microfluidics and Nanofluidics

, Volume 19, Issue 1, pp 9–18 | Cite as

Direct, one-step molding of 3D-printed structures for convenient fabrication of truly 3D PDMS microfluidic chips

  • Ho Nam Chan
  • Yangfan Chen
  • Yiwei Shu
  • Yin Chen
  • Qian Tian
  • Hongkai WuEmail author
Research Paper


In this work, we developed a convenient, one-step soft-lithographic-based molding technique for molding truly 3D microfluidic channels in polydimethylsiloxane (PDMS) by overcoming two grand challenges. We optimized the post-treatment conditions for 3D-printed resin structures to facilitate the use of them as masters for PDMS replica molding. What is more important, we demonstrated a novel method for single-step molding from 3D-printed microstructures to generate truly 3D microfluidic networks easily. With this technique, we fabricated some key, functional 3D microfluidic structures and components including a basket-weaving network, a 3D chaotic advective mixer and microfluidic peristaltic valves. Furthermore, an interesting “injection-on-demand” microfluidic device was also demonstrated. Our technique offers a simple, fast route to the fabrication of 3D microfluidic chips in a short time without clean-room facilities.


3D printing Soft lithography One-step molding 3D microfluidic chip PDMS 



The authors are grateful for the funding provided by Hong Kong Research Grants Council (#605210, #604712 and CUHK4/CRF/12G).

Supplementary material

10404_2014_1542_MOESM1_ESM.docx (1.2 mb)
Supplementary material 1 (DOCX 1271 kb)

Supplementary material 2 (MPG 24392 kb)

Supplementary material 3 (MPG 3296 kb)

Supplementary material 4 (MPG 1366 kb)


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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Ho Nam Chan
    • 1
  • Yangfan Chen
    • 1
  • Yiwei Shu
    • 1
  • Yin Chen
    • 1
  • Qian Tian
    • 1
  • Hongkai Wu
    • 1
    Email author
  1. 1.Department of ChemistryHong Kong University of Science and TechnologyClear Water Bay, KowloonChina

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