Advertisement

Microfluidics and Nanofluidics

, Volume 17, Issue 6, pp 1025–1037 | Cite as

Numerical modeling of ultrasonic particle manipulation for microfluidic applications

  • Süleyman Büyükkoçak
  • Mehmet Bülent Özer
  • Barbaros Çetin
Research Paper

Abstract

A numerical simulation methodology for ultrasonic particle/cell separation and cell washing processes is introduced and validated by comparing with the results from the literature. In this study, a finite element approach is used for modeling fluid flow in a microchannel and analytical relations are utilized for the calculation of the ultrasonic radiation forces. The solutions in acoustic and fluidic domains are coupled, and the particle separation under the influence of ultrasonic waves is numerically simulated. In order to simulate the cell washing process, diffusion and fluid dynamics solutions are coupled and solved. A Monte Carlo approach is chosen where statistical distributions are implemented in the simulations. Uniform distributions for the starting locations of particles/cells in the microchannel and normal distributions for the size of the particles are used in numerical simulations. In each case, 750 particles are used for the simulation, and the performance of separation process is evaluated by checking how many microparticles resulted in the targeted outlet channels. Channel geometries for the numerical simulations are adapted from the experimental studies in literature, and comparison between the reported experimental results and the numerical estimations is performed. It has been observed that the numerical estimations and experimental results from the literature are in good agreement, and the proposed methodology may be implemented as a design tool for ultrasonic particle manipulation for microfluidic applications.

Keywords

Microfluidics Acoustophoresis Particle separation Acoustic radiation force Acoustic standing wave 

Notes

Acknowledgments

Financial support from the Turkish Scientific and Technical Research Council, Grant No. 112M102, is greatly appreciated.

References

  1. Adams JD, Soh HT (2010) Tunable acoustophoretic band-pass particle sorter. Appl Phys Lett 97Google Scholar
  2. Bazou D, Castro A, Hoyos M (2012) Controlled cell aggregation in a pulsed acoustic field. Ultrasonics 52(7):842–850CrossRefGoogle Scholar
  3. Bhagat lAAS, Papautsky I, (2008) Enhancing particle dispersion in a passive planar micromixer using rectangular obstacles. J Micromech Microeng 18:1–9Google Scholar
  4. Dron O, Ratier C, Hoyos M, Aider J-L (2009) Parametric study of acoustic focusing of particles in a micro-channel in the perspective to improve micro-PIV measurements. Microfluid Nanofluid 7:857–867CrossRefGoogle Scholar
  5. Evander M, Johansson L, Lilliehorn T, Piskur J, Lindvall M, Johansson S, Almqvist M, Laurell T, Nilsson J (2007) Noninvasive acoustic cell trapping in a microfluidic perfusion system for online bioassays. Anal Chem 79:2984–2991CrossRefGoogle Scholar
  6. Glynne-Jones P, Mishra PP, Boltryk RJ, Hill M (2013) Efficient finite element modeling of radiation forces on elastic particles of arbitrary size and geometry. J Acoust Soc Am 133:1885–1893CrossRefGoogle Scholar
  7. Gorkov LP (1962) On the forces acting on a small particle in an acoustic field in an ideal fluid. Sov Phys Doklady 6:773–776Google Scholar
  8. Gralinski I, Alan T, Neild A (2012) Non-contact acoustic trapping in circular cross-section glass capillaries: a numerical study. J Acoust Soc Am 132:2978–2987CrossRefGoogle Scholar
  9. Haake A, Neild A, Kim D, Ihm J, Sun Y, Dual J, Ju B (2005) Manipulation of cells using an ultrasonic pressure field. Ultrasound Med Biol 31:857–864CrossRefGoogle Scholar
  10. Hawkes JJ, Barrow D, Coakley WT (1998) Micro-particle manipulation in millimetre scale ultrasonic standing wave chambers. Ultrasonics 36:925–931CrossRefGoogle Scholar
  11. Hawkes JJ, Barber RW, Emerson DR, Coakley WT (2004) Continuous cell washing and mixing driven by an ultrasound standing wave within a microfluidic channel. Lab Chip 4:446–452CrossRefGoogle Scholar
  12. Johnson DA, Feke DL (1995) Methodology for fractionating suspended particles using ultrasonic standing wave and divided flow fields. Separ Technol 5:251–258CrossRefGoogle Scholar
  13. Kumar M, Feke DL, Belovich JM (2005) Fractionation of cell mixtures using acoustic and laminar flow fields. Biotechnol Bioeng 89. doi: 10.1002/bit.20294
  14. Limaye S, Coakley WT (1998) Clarification of small volume microbial suspensions in an ultrasonic standing wave. J Appl Microbiol 84:1035–1042CrossRefGoogle Scholar
  15. Martinez-Duarte R, Gorkin RA III, Abi-Samra K, Madou MJ (2010) The integration of 3D carbone-electrode dielectrophoresis on a CD-like centrifugal microfluidic platform. Lab Chip 10:1030–1043CrossRefGoogle Scholar
  16. Nam J, Lee Y, Shin S (2011) Size-dependent microparticles separation through standing surface acoustic waves. Microfluid Nanofluid 11:317–326CrossRefGoogle Scholar
  17. Neild A, Oberti S, Haake A, Dual J (2006) Finite element modeling of a micro-particle manipulator. Ultrasonics 44:455–460CrossRefGoogle Scholar
  18. Neild A, Oberti S, Dual J (2007) Design, modeling and characterization of microfluidic devices for ultrasonic manipulation. Sens Actuators B 121:452–461CrossRefGoogle Scholar
  19. Petersson F, Nilsson A, Holm C, Jnsson H, Laurell T (2005a) Continuous separation of lipid particles from erythrocytes by means of laminar flow and acoustic standing wave forces. Lab Chip 5:20–22CrossRefGoogle Scholar
  20. Petersson F, Nilsson A, Jonsson H, Laurell T (2005b) Carrier medium exchange through ultrasonic particle switching in microfluidic channels. Anal Chem 77:1216–1221CrossRefGoogle Scholar
  21. Petersson F, Berg LA, Sward-Nilsson A, Laurell T (2007) Free flow acoustophoresis: microfluidic-based mode of particle and cell separation. Anal Chem 79:5117–5123CrossRefGoogle Scholar
  22. Settnes M, Bruus H (2012) Forces acting on a small particle in an acoustic field in a viscous fluid. Phys Rev E 85Google Scholar
  23. Shi J, Huang H, Stratton Z, Huang Y, Huang TJ (2009) Continuous particle separation in a microfluidic channel via standing surface acoustic waves (SSAW). Lab Chip 9:3354–3359CrossRefGoogle Scholar
  24. Smith DM, Wiggins TA (1972) Sound speeds and laser induced damage in polystyrene. Appl Optics 11:2681Google Scholar
  25. Townsend RJ, Hill M, Harris NR, White NM (2004) Modeling of particle paths passing through an ultrasonic standing wave. Ultrasonics 42:319–324CrossRefGoogle Scholar
  26. Tripp G, Ventikos Y, Taggart DP, Coussios C-C (2011) CFD modeling of an ultrasonic separator for the removal of lipid particles from pericardial suction blood. IEEE Trans Biomed Eng 58:282–290CrossRefGoogle Scholar
  27. Trujillo FJ, Eberhardt S, Möller D, Dual J, Knoerzer K (2013) Multiphysics modelling of the separation of suspended particles via frequency ramping of ultrasonic standing waves. Ultrason Sonochem 20:655–666CrossRefGoogle Scholar
  28. Yosioka K, Kawasima Y (1955) Acoustic radiation pressure on a compressible sphere. Acoustica 5:167–173Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Süleyman Büyükkoçak
    • 1
  • Mehmet Bülent Özer
    • 1
  • Barbaros Çetin
    • 2
  1. 1.Department of Mechanical EngineeringTOBB University of Economics and TechnologyAnkaraTurkey
  2. 2.Microfluidics and Lab-on-a-chip Research Group, Mechanical Engineering Departmentİhsan Doğramacı  Bilkent UniversityAnkaraTurkey

Personalised recommendations