Microfluidics and Nanofluidics

, Volume 16, Issue 5, pp 895–905 | Cite as

Centrifugal automation of a triglyceride bioassay on a low-cost hybrid paper-polymer device

  • Neus Godino
  • Elizaveta Vereshchagina
  • Robert GorkinIII
  • Jens Ducrée
Research Paper


We present a novel paper-polymer hybrid construct for the simple automation of fundamental microfluidic operations in a lab-on-a-disc platform. The novel design, we term a paper siphon, consists of chromatographic paper strips embedded along a siphon microchannel. The paper siphon relies on two main interplaying forces to create unique valving and liquid-sampling methods in centrifugal microfluidics. At sufficiently low speeds, the inherent wicking of the paper overcomes the rotationally induced centrifugal force to drive liquids towards inwards positions of the disc. At elevated speeds, the dominant centrifugal force will extract liquid from the siphon paper strip towards the edge of the disc. Distinct modes of flow control have been developed to account for water (reagent) and more viscous plasma samples. The system functionality is demonstrated by the automation of sequential sample preparation steps in a colorimetric triglyceride assay: plasma is metered from a whole blood sample and incubated with a specific enzymatic mixture, followed by detection of triglyceride levels through (off-disc) absorbance measurements. The successful quantification of triglycerides and the simple fabrication offer attractive directions for such hybrid devices in low-cost bioanalysis.


Lab-on-a-disc Centrifugal microfluidics Paper microfluidics Point of care diagnostic Triglyceride assay 



This work has been supported in part by the FP-7 ENIAC programme CAJAL4EU, Enterprise Ireland under Grant No. IR/2010/0002 and the Science Foundation of Ireland (Grant No. 10/CE/B1821).


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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Neus Godino
    • 1
    • 2
  • Elizaveta Vereshchagina
    • 1
    • 3
  • Robert GorkinIII
    • 1
    • 4
  • Jens Ducrée
    • 1
  1. 1.Biomedical Diagnostics Institute, National Centre for Sensor Research, School of Physical SciencesDublin City UniversityDublinIreland
  2. 2.Fraunhofer Institute for Biomedical Engineering IBMTPotsdamGermany
  3. 3.Microsystems Centre of Tyndall National InstituteCorkIreland
  4. 4.ARC Centre of Excellence for Electromaterials Science, Intelligent Polymer Research InstituteUniversity of WollongongWollongongAustralia

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