Centrifugal automation of a triglyceride bioassay on a low-cost hybrid paper-polymer device
- 795 Downloads
We present a novel paper-polymer hybrid construct for the simple automation of fundamental microfluidic operations in a lab-on-a-disc platform. The novel design, we term a paper siphon, consists of chromatographic paper strips embedded along a siphon microchannel. The paper siphon relies on two main interplaying forces to create unique valving and liquid-sampling methods in centrifugal microfluidics. At sufficiently low speeds, the inherent wicking of the paper overcomes the rotationally induced centrifugal force to drive liquids towards inwards positions of the disc. At elevated speeds, the dominant centrifugal force will extract liquid from the siphon paper strip towards the edge of the disc. Distinct modes of flow control have been developed to account for water (reagent) and more viscous plasma samples. The system functionality is demonstrated by the automation of sequential sample preparation steps in a colorimetric triglyceride assay: plasma is metered from a whole blood sample and incubated with a specific enzymatic mixture, followed by detection of triglyceride levels through (off-disc) absorbance measurements. The successful quantification of triglycerides and the simple fabrication offer attractive directions for such hybrid devices in low-cost bioanalysis.
KeywordsLab-on-a-disc Centrifugal microfluidics Paper microfluidics Point of care diagnostic Triglyceride assay
This work has been supported in part by the FP-7 ENIAC programme CAJAL4EU, Enterprise Ireland under Grant No. IR/2010/0002 and the Science Foundation of Ireland (Grant No. 10/CE/B1821).
- Ballerini DR, Li X, Shen W (2012) Patterned paper and alternative materials as substrates for low-cost microfluidic diagnostics. Microfluid Nanofluidics :769–787. doi: 10.1007/s10404-012-0999-2
- Czugala M, Gorkin R III, Phelan T, Gaughran J, Curto VF, Ducrée J, Diamond D, Benito-López F (2012) Optical sensing system based on wireless paired emitter detector diode device and ionogels for lab-on-a-disc water quality analysis. Lab Chip 12:5069–5078. doi: 10.1039/c2lc40781g CrossRefGoogle Scholar
- Fu E, Liang T, Spicar-Mihalic P, Houghtaling J, Ramachandran S, Yager P (2012) Two-dimensional paper network format that enables simple multistep assays for use in low-resource settings in the context of malaria antigen detection. Anal Chem 84:4574–4579. doi: 10.1021/ac300689s CrossRefGoogle Scholar
- Godino N, Comaskey E, Gorkin R, Ducrée JD (2012a) Centrifugally enhanced paper microfluidics. In: The 25th International Conference on Micro Electro Mechanical Systems, 29 January–2 February 2012, Paris, France, p 1017–1020Google Scholar
- Godino N, Vereshchagina E, Gorkin R, Ducrée J (2012b) Hybrid paper-polymer lab-on-a-disc for bioassay integration. In: Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS 2012) 0:1369–1371Google Scholar
- Lowe GDO, Barbebel JC (1988) Plasma and blood viscosity. Clin Blood Rheol 1:11–44Google Scholar
- Pelton R (2009) Bioactive paper provides a low-cost platform for diagnostics. Trends Anal Chem 28:925–942Google Scholar
- Vereshchagina E, Bourke K, Meehan L, Dixit C, Glade DM, Ducrée J (2012) Multi-material paper-disc devices for low cost biomedical diagnostics. In: The proceedings of the 26th International Conference on Micro Electro Mechanical Systems, 20–24 January, TaipeiGoogle Scholar