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Microfluidics and Nanofluidics

, Volume 13, Issue 4, pp 675–681 | Cite as

Rotationally controlled magneto-hydrodynamic particle handling for bead-based microfluidic assays

  • Robert Burger
  • Patrick Reith
  • Victor Akujobi
  • Jens Ducrée
Research Paper

Abstract

This work presents a novel magnetic actuation scheme for advanced particle handling on our previously introduced, centrifugal microfluidic platform for array-based analysis of individual cells and beads. The conceptually simple actuation is based on the reciprocating motion of an elastomeric membrane featuring an integrated permanent magnet and a stationary magnet aligned along the orbit of a disc-based chamber. This compression chamber is placed at the downstream end of the particle capture chamber to induce centripetally directed, hydrodynamic lift forces on particles trapped in V-shaped geometrical barriers. Towards high frequencies of rotation, the on-disc magnet ceases to follow the rapidly oscillating magnetic field, so that the magnetic actuator is disabled during the initial, sedimentation-based filling of the trap array. At reduced spin speeds, the residence time of the magnetic actuator is sufficient to displace the magnetic actuator, resulting in a flow through the V-cup array that re-distributes, and eventually fully depletes, the previously trapped beads from the array. The same magnetic deflection scheme is also demonstrated to accelerate mixing, e.g. for upstream sample preparation.

Keywords

PDMS Spindle Motor Potassium Thiocyanate Artificial Gravity Magnetic Actuator 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This work was supported by the Science Foundation Ireland under Grant No. 10/CE/B1821.

Supplementary material

10404_2012_994_MOESM1_ESM.docx (344 kb)
Supplementary material 1 (DOCX 343 kb)

Supplementary material 2 (M4V 34054 kb)

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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Robert Burger
    • 1
  • Patrick Reith
    • 1
  • Victor Akujobi
    • 1
  • Jens Ducrée
    • 1
  1. 1.Biomedical Diagnostics Institute, National Centre for Sensor Research, School of Physical SciencesDublin City UniversityDublinIreland

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