Microfluidics and Nanofluidics

, Volume 10, Issue 3, pp 685–690 | Cite as

Laser microstructuration of three-dimensional enzyme reactors in microfluidic channels

  • Monica Iosin
  • Teodora Scheul
  • Clément Nizak
  • Olivier Stephan
  • Simion Astilean
  • Patrice Baldeck
Short Communication


In this paper, we report on the fabrication of three-dimensional (3D) enzymatic microreactors within polydimethylsiloxane microfluidic channels through a photocrosslinking mechanism mediated by the two-photon absorption process at the focal point of pulse lasers, i.e., a sub-nanosecond Nd:YAG microlaser or a femtosecond Ti:Sapphire laser. This approach allows the building of localized 3D trypsin structures with submicron resolution. The fabrication of two different trypsin structures was successfully demonstrated using Eosin Y and Flavin Adenine Dinucleotide as biological photosensitizers: (i) arrays of 3D cylindrical rows and (ii) 3D woodpile structure. The enzymatic activity of the fabricated structures was evaluated by fluorescence spectroscopy using BODIPY FL casein as fluorogenic substrate. The real time investigation of the peptide cleavage into the microfluidic channel demonstrated that the fabricated trypsin microstructures maintain their catalytic activity. This approach opens up the way to complex multistep enzymatic reactions in well-localized regions of microfluidic devices, with great importance in health screening and biomedical diagnostics.


Laser microfabrication Two-photon absorption Microreactors Enzymatic activity Microfluidic channels 



This work was supported by Agence Universitaire de la Francophonie (AUF).


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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Monica Iosin
    • 1
    • 2
  • Teodora Scheul
    • 1
    • 2
  • Clément Nizak
    • 2
  • Olivier Stephan
    • 2
  • Simion Astilean
    • 1
  • Patrice Baldeck
    • 2
  1. 1.Nanobiophotonics Laboratory, Institute for Interdisciplinary Experimental ResearchBabes-Bolyai UniversityCluj-NapocaRomania
  2. 2.Laboratoire de Spectrométrie PhysiqueUniversité Joseph FourierSaint Martin d’Hères CedexFrance

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