Microfluidics and Nanofluidics

, Volume 10, Issue 1, pp 59–67

Analysis of sperm concentration and motility in a microfluidic device

  • Yu-An Chen
  • Zi-Wei Huang
  • Fang-Sheng Tsai
  • Chang-Yu Chen
  • Cheng-Ming Lin
  • Andrew M. Wo
Research Paper

Abstract

A home-use device that allows rapid and quantitative sperm quality analysis is desirable but not yet fully realized. To aid this effort, this article presents a microfluidic device capable of quantifying sperm quality in terms of two critical fertility-related parameters—motile sperm concentration and motility. The microdevice produces flow field for sperms to swim against, and sperms that overcame the flow within a specified time are propelled along in a separate channel and counted via resistive pulse technique. Data are compared to two control methods clinically utilized for sperm quality exam—hemocytometer and the sperm quality analyzer. Results reveal the numbers of pulses generated by passage of sperms correlates strongly with the two control methods: pulse number from 0 to 335 corresponds to progressively motile sperm concentrations from 0 to 19 × 106/ml (hemocytometer) and Sperm Motility Index from 0 to 204 (sperm quality analyzer). The microdevice should be applicable to facilitate self-assessment of sperm quality at home.

Keywords

Microfluidics Sperm Infertility Resistive pulse technique 

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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Yu-An Chen
    • 1
  • Zi-Wei Huang
    • 1
  • Fang-Sheng Tsai
    • 2
  • Chang-Yu Chen
    • 1
  • Cheng-Ming Lin
    • 1
  • Andrew M. Wo
    • 1
  1. 1.Institute of Applied MechanicsNational Taiwan UniversityTaipei 106Taiwan, ROC
  2. 2.Department of UrologyNational Taiwan University HospitalTaipeiTaiwan, ROC

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