Microfluidics and Nanofluidics

, Volume 9, Issue 2–3, pp 411–426

Dielectrophoretic-activated cell sorter based on curved microelectrodes

  • Khashayar Khoshmanesh
  • Chen Zhang
  • Francisco J. Tovar-Lopez
  • Saeid Nahavandi
  • Sara Baratchi
  • Arnan Mitchell
  • Kourosh Kalantar-Zadeh
Research Paper
  • 1.3k Downloads

Abstract

This article presents the numerical and experimental analysis of a dielectrophoretic-activated cell sorter (DACS), which is equipped with curved microelectrodes. Curved microelectrodes offer unique advantages, since they create strong dielectrophoretic (DEP) forces over the tips and maintain it over a large portion of their structure, as predicted by simulations. The performance of the system is assessed using yeast (Saccharomyces cerevisiae) cells as model organisms. The separation of the live and dead cells is demonstrated at different medium conductivities of 0.001 and 0.14 S/m, and the sorting performance was assessed using a second array of microelectrodes patterned downstream the microchannel. Further, microscopic cell counting analysis reveals that a single pass through the system yields a separating efficiency of ~80% at low medium conductivities and ~85% at high medium conductivities.

Keywords

Dielectrophoresis Microfluidics Yeast Sorting 

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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Khashayar Khoshmanesh
    • 1
  • Chen Zhang
    • 2
  • Francisco J. Tovar-Lopez
    • 2
  • Saeid Nahavandi
    • 1
  • Sara Baratchi
    • 3
  • Arnan Mitchell
    • 2
  • Kourosh Kalantar-Zadeh
    • 2
  1. 1.Centre for Intelligent Systems ResearchDeakin UniversityGeelongAustralia
  2. 2.School of Electrical and Computer EngineeringRMIT UniversityMelbourneAustralia
  3. 3.Institute of Biotechnology (BioDeakin)Deakin UniversityGeelongAustralia

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