Milliseconds microfluidic chaotic bubble mixer

  • Xiaole Mao
  • Bala Krishna Juluri
  • Michael Ian Lapsley
  • Zackary Stoeri Stratton
  • Tony Jun Huang
Short Communication

Abstract

In this study, we report a rapid microfluidic mixing device based on chaotic advection induced by microbubble–fluid interactions. The device includes inlets for to-be-mixed fluids and nitrogen gas. A side-by-side laminar flow segmented by monodisperse microbubbles is generated when the fluids and the nitrogen are co-injected through a flow focusing micro-orifice. The flow subsequently enters a series of hexagonal expansion chambers, in which the hydrodynamic interaction among the microbubbles results in the stretch and fold of segmented fluid volumes and rapid mixing and homogenization. We characterize the performance of the microfluidic mixer and demonstrate rapid mixing within 20 ms. We further show that bubbles can be conveniently removed from the mixed fluids using a microfluidic comb structure on completion of the mixing.

Keywords

Chaotic advection Microbubble Microfluidics Rapid mixing 

Notes

Acknowledgments

Authors thank Daniel Ahmed and Aitan Lawit for help in the manuscript preparation. This research was supported by National Science Foundation (ECCS-0824183 and ECCS-0801922) and the Penn State Center for Nanoscale Science (MRSEC). Components of this study were conducted at the Penn State node of the NSF-funded National Nanotechnology Infrastructure Network (NNIN).

References

  1. Biddiss E, Erickson D, Li D (2004) Heterogeneous surface charge enhanced micromixing for electrokinetic flows. Anal Chem 76:3208–3213CrossRefGoogle Scholar
  2. Chang CC, Yang RJ (2007) Electrokinetic mixing in microfluidic systems. Microfluid Nanofluid 3:501–525CrossRefMathSciNetGoogle Scholar
  3. de Mello J, de Mello A (2004) Focus microscale reactors: nanoscale products. Lab Chip 4:11N–15NCrossRefGoogle Scholar
  4. Floyd-Smith TM, Golden JP, Howell PB, Ligler FS (2006) Characterization of passive microfluidic mixers fabricated using soft lithography. Microfluid Nanofluid 2:180CrossRefGoogle Scholar
  5. Garstecki P, Fischbach MA, Whitesides GM (2005) Design for mixing using bubbles in branched microfluidic channels. Appl Phys Lett 86:244108CrossRefGoogle Scholar
  6. Garstecki P, Fuerstman MJ, Fischbach MA, Sia SK, Whitesides GM (2006) Mixing with bubbles: a practical technology for use with portable microfluidic devices. Lab Chip 6:207–212CrossRefGoogle Scholar
  7. Günther A, Khan SA, Thalmann M, Trachsel F, Jensen KF (2004) Transport and reaction in microscale segmented gas–liquid flow. Lab Chip 4:278–286CrossRefGoogle Scholar
  8. Hardt S, Drese KS, Hessel V, Schönfeld F (2005) Passive micromixers for applications in the microreactor and μTAS fields. Microfluid Nanofluid 1:108CrossRefGoogle Scholar
  9. Hosokawa K, Fujii T, Endo I (1999) Handling of picoliter liquid samples in a poly(dimethylsiloxane)-based microfluidic device. Anal Chem 71:4781–4785CrossRefGoogle Scholar
  10. Kang T, Singh M, Kwon T, Anderson P (2008) Chaotic mixing using periodic and aperiodic sequences of mixing protocols in a micromixer. Microfluid Nanofluid 4:589–599CrossRefGoogle Scholar
  11. Knight JB, Vishwanath A, Brody JP, Austin RH (1998) Hydrodynamic focusing on a silicon chip: mixing nanoliters in microseconds. Phys Rev Lett 80:3863–3866CrossRefGoogle Scholar
  12. Liu RH, Stremler MA, Sharp KV, Olsen MG, Santiago JG, Adrian RJ, Aref H, Beebe DJ (2000) Passive mixing in a three-dimensional serpentine microchannel. J Microelectromech Syst 9:190–197CrossRefGoogle Scholar
  13. Mao X, Waldeisen JR, Huang TJ (2007a) “Microfluidic drifting”—implementing three-dimensional hydrodynamic focusing with a single-layer planar microfluidic device. Lab Chip 7:1260–1262CrossRefGoogle Scholar
  14. Mao X, Waldeisen JR, Juluri BK, Huang TJ (2007b) Hydrodynamically tunable optofluidic cylindrical microlens. Lab Chip 7:1303–1308CrossRefGoogle Scholar
  15. Mao X, Lin SS, Dong C, Huang TJ (2009a) Single-layer planar on-chip flow cytometer using microfluidic drifting based three-dimensional (3D) hydrodynamic focusing. Lab Chip 9:1583–1589CrossRefGoogle Scholar
  16. Mao X, Lin SS, Lapsley MI, Shi J, Juluri BK, Huang TJ (2009b) Tunable liquid gradient refractive index (L-GRIN) lens with two degrees of freedom. Lab Chip 9:2050–2058CrossRefGoogle Scholar
  17. Miller EM, Wheeler AR (2008) A digital microfluidic approach to homogeneous enzyme assays. Anal Chem 80:1614–1619CrossRefGoogle Scholar
  18. Ottino JM (1989) The kinematics of mixing: stretching, chaos, and transport. Cambridge University Press, CambridgeMATHGoogle Scholar
  19. Ottino JM (1994) Mixing and chemical reactions a tutorial. Chem Eng Sci 49:4005–4027CrossRefGoogle Scholar
  20. Ottino JM, Wiggins S (2004) Designing optimal micromixers. Science 305:485–486CrossRefGoogle Scholar
  21. Park HY, Qiu X, Rhoades E, Korlach J, Kwok LW, Zipfel WR, Webb WW, Pollack L (2006) Achieving uniform mixing in a microfluidic device: hydrodynamic focusing prior to mixing. Anal Chem 78:4465–4473CrossRefGoogle Scholar
  22. Pollack L, Tate MW, Darnton NC, Knight JB, Gruner SM, Eaton WA, Austin RH (1999) Compactness of the denatured state of a fast-folding protein measured by submillisecond small-angle x-ray scattering. Proc Natl Acad Sci USA 96:10115–10117CrossRefGoogle Scholar
  23. Puleo CM, Wang T-H (2009) Microfluidic means of achieving attomolar detection limits with molecular beacon probes. Lab Chip. doi: 10.1039/b819605b
  24. Schonfeld F, Hessel V, Hofmann C (2004) An optimised split-and-recombine micro-mixer with uniform ‘chaotic’ mixing. Lab Chip 4:65–69CrossRefGoogle Scholar
  25. Shi J, Mao X, Ahmed D, Colletti A, Huang TJ (2008) Focusing microparticles in a microfluidic channel with standing surface acoustic waves (SSAW). Lab Chip 8:221–223CrossRefGoogle Scholar
  26. Song H, Ismagilov RF (2003) Millisecond kinetics on a microfluidic chip using nanoliters of reagents. J Am Chem Soc 125:14613–14619CrossRefGoogle Scholar
  27. Song H, Bringer MR, Tice JD, Gerdts CJ, Ismagilov RF (2003) Experimental test of scaling of mixing by chaotic advection in droplets moving through microfluidic channels. Appl Phys Lett 83:4664–4666CrossRefGoogle Scholar
  28. Stroock AD, Dertinger SKW, Ajdari A, Mezic I, Stone HA, Whitesides GM (2002) Chaotic mixer for microchannels. Science 295:647–651CrossRefGoogle Scholar
  29. Therriault D, White SR, Lewis JA (2003) Chaotic mixing in three-dimensional microvascular networks fabricated by direct-write assembly. Nat Mater 2:265–271CrossRefGoogle Scholar
  30. Wu Z, Nguyen N-T (2005) Convective–diffusive transport in parallel lamination micromixers. Microfluid Nanofluid 1:208–217CrossRefGoogle Scholar
  31. Yang J-T, Huang K-J, Lin Y-C (2005) Geometric effects on fluid mixing in passive grooved micromixers. Lab Chip 5:1140–1147CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Xiaole Mao
    • 1
    • 2
  • Bala Krishna Juluri
    • 1
  • Michael Ian Lapsley
    • 1
  • Zackary Stoeri Stratton
    • 1
  • Tony Jun Huang
    • 1
    • 2
  1. 1.Department of Engineering Science and MechanicsPennsylvania State UniversityUniversity ParkUSA
  2. 2.Department of BioengineeringPennsylvania State UniversityUniversity ParkUSA

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