Microfluidics and Nanofluidics

, Volume 5, Issue 5, pp 689–694 | Cite as

Fluorescence detection in a micro flow cytometer without on-chip fibers

Short Communication

Abstract

This paper describes a novel concept of integrated on-chip fiber free laser-induced fluorescence detection system. The poly-dimethylsiloxane (PDMS) chip was fabricated using soft lithography and was bonded with a glass substrate of 150 μm thickness that reduced the distance of channel-to-sidewall to less than 180 μm. The cells and particles detection was conducted by an external single fiber close to the glass substrate that transmitted laser light for simultaneous excitation and receipt of the emission light signals. The performance of the proposed device was demonstrated using fluorescence beads, stained white blood cells, and yeast cells. The experimental results showed the simplicity and flexibility of the proposed device configuration which can provide convenient on-chip integration interface for fast, high throughput, and low-cost laser-induced fluorescence detection micro flow cytometer.

Keywords

Microchip Micro flow cytometer Microfluidic PDMS 

References

  1. Ateya DA, Erickson JS, Howell PB Jr, Hilliard LR, Golden JP, Ligler FS (2008) The good, the bad, the tiny: a review of microflow cytometry. Anal Bioanal Chem. doi:10.1007/S00216-007-1827-5
  2. Cui L, Zhang T, Morgan H (2002) Optical particle detection integrated in a dielectrophoretic lab-on-a-chip. J Micromech Microeng 12:7–12CrossRefGoogle Scholar
  3. Duffy DC, McDonald JC, Schueller OJA, Whitesides GM (1998) Rapid prototyping of microfluidic systems in Poly(dimethylsiloxane). Anal Chem 70(23):4974–4984CrossRefGoogle Scholar
  4. Erickson D, Li D (2004) Integrated microfluidic devices. Anal Chim Acta 507:11–26CrossRefGoogle Scholar
  5. Ferris MM, McCabe MO, Doan LG, Rowlen KL (2002) Rapid enumeration of respiratory viruses. Anal Chem 74:1849–1856CrossRefGoogle Scholar
  6. Figeys D, Aebersold R (1998) High sensitivity analysis of proteins and peptides by capillary electrophoresis-tandem mass spectrometry: recent developments in technology and applications. Electrophoresis 19:885–892CrossRefGoogle Scholar
  7. Fu L-M, Yang R-J, Lin C-H, Pan Y-J, Lee G-B (2004) Electrokinetically driven micro flow cytometers with integrated fiber optics for on-line cell/particle detection. Anal Chim Acta 507:163–169CrossRefGoogle Scholar
  8. Holmes D, Morgan H, Green NG (2006) High throughput analysis: Combining dielectrophoretic particle focusing with confocal optical detection. Biosens Bioelectron 21:1621–1630CrossRefGoogle Scholar
  9. Huh D, Tung Y-C, Wei H-H, Grotberg JB, Zhang M, Skerlos SJ, Kurabayashi K, Takayama S (2002) Use of air-liquid two-phase flow in hydrophobic microfluidic channels for disposable flow cytometers. Biomed Microdevices 4(2):141–149CrossRefGoogle Scholar
  10. Huh D, Gu W, Kamotani Y, Grotberg JB, Takayama S (2005) Misrofluidics for flow cytometric analysis of cells and particles. Physiol Meas 26:R73–R98CrossRefGoogle Scholar
  11. Lin C-H, Lee G-B, Fu L-M, Hwey B-H (2004) Vertical focusing device utilizing dielectrophoretic force and its application on microflow cytometer. J Microelectromech Syst 13:923–932CrossRefGoogle Scholar
  12. McClain MA, Culbertson CT, Jacobson SC, Ramsey JM (2001) Flow cytometry of Escherichia coli on microfluidic devices. Anal Chem 73:5334–5338CrossRefGoogle Scholar
  13. Mogensen KB, Klank H, Kutter JP (2004) Recent developments in detection for microfluidic systems. Electrophoresis 25:3498–3512CrossRefGoogle Scholar
  14. Morgan H, Holmes D, Green NG (2006) High speed simultaneous single particle impedance and fluorescence analysis on a chip. Curr Appl Phys 6:367–370CrossRefGoogle Scholar
  15. Pamme N, Koyama R, Manz A (2003) Counting and sizing of particle agglomerates in a microfluidic device using laser light scattering: application to a particle-enchanced immunoassay. Lab Chip 3:187–192CrossRefGoogle Scholar
  16. Sun T, Holmes D, Gawad S, Green NG, Morgan H (2007) High speed multi-frequency impedance analysis of single particles in a microfuidic cytometer using maximum length sequences. Lab Chip 7:1034–1040CrossRefGoogle Scholar
  17. Trumbull JD, Glasgow IK, Beebe DJ, Magin RL (2000) Integrating microfabricated fluidic systems and NMR spectroscopy. IEEE Trans Biomed Eng 47:3–7CrossRefGoogle Scholar
  18. Tung Y-C, Zhang M, Lin C-T, Kurabayashi K, Skerlos SJ (2004) PDMS-based opto-fluidic micro flow cytometer with two-color, multi-angle fluorescence detection capability using PIN photodiodes. Sens Actuators B 98:356–367CrossRefGoogle Scholar
  19. Xu D, Kang Y, Sridhar M, Hmelo AB, Feldman LC, Li D, Li D (2007) Wide-spectrum ultrasensitive fluidic sensors with amplification from both fluidic circuits and metal oxide semiconductor field effect transistors. Appl Phys Lett 91:013901CrossRefGoogle Scholar
  20. Yao B, Luo G-A, Feng X, Wang W, Chen L-X, Wang Y-M (2004) A microfluidic device based on gravity and electric force driving for flow cytometry and fluorescence activated cell sorting. Lab Chip 4:603–607CrossRefGoogle Scholar
  21. Yi C, Zhang Q, Li C-W, Yang J, Zhao J, Yang M (2006) Optical and electrochemical detection techniques for cell-based microfluidic systems. Anal Bioanal Chem 384:1259–1268CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  1. 1.Department of Mechanical EngineeringNational Cheng-Kung UniversityTainanTaiwan

Personalised recommendations