Fabrication of bio/nano interfaces between biological cells and carbon nanotubes using dielectrophoresis
The authors have previously demonstrated the manipulation of bacteria and carbon nanotubes (CNTs) using dielectrophoresis (DEP) and its application for various types of biological and chemical sensors. This paper demonstrates simultaneous DEP handling of bacteria and CNTs, which are mixed and suspended in water. The CNTs were solubilized in water using microplasma-based treatment. When a microelectrode was energized with an ac voltage in the suspension of Escherichia coli (E. coli) cells and multi-walled CNTs (MWCNTs), both of them were simultaneously trapped in the microelectrode gap. Scanning electron microscopy (SEM) images revealed that E. coli cells were trapped on the surface or the tip of MWCNTs, where the electric field strength was intensified due to high aspect ratio of MWCNTs. As a result, bio/nano interfaces between bacteria and MWCNTs were automatically formed in a self-assembly manner. A potential application of the DEP-fabricated bio/nano interfaces is a drug delivery system (DDS), which is realized by transporting drug molecules from CNTs to cells across the cell membrane, which can be electroporated by the local high electric field formed on the CNT surface.
KeywordsDielectrophoresis Carbon nanotube Solubilization Biological cell Drug delivery system (DDS)
- Imasaka K, Suehiro J, Kato Y (2007) Enhancement of microplasma-based water-solubilization of single-walled carbon nanotubes using gas bubbling in water. Nanotechnology 18, Paper No.335602Google Scholar
- Pethig R (2007) BioMEMS and biomedical nanotechnology, vol II: micro/nano technologies for genomics and proteomics. Springer, USGoogle Scholar