Microfluidics and Nanofluidics

, Volume 3, Issue 2, pp 217–225 | Cite as

Lab-on-a-display: a new microparticle manipulation platform using a liquid crystal display (LCD)

Research Paper

Abstract

This paper reports a new portable microfluidic platform, “lab-on-a-display,” that microparticles are manipulated by optoelectronic tweezers (OET) on a liquid crystal display (LCD). The OET has been constructed by assembling a ground layer, a liquid chamber, and a photoconductive layer. Without lens or optical alignments, the LCD image directly forms virtual electrodes on the photoconductive layer for dielectrophoretic manipulation. The lab-on-a-display was first realized by a conventional monochromatic LCD module and a light source brighter than 5,000 lux. It was successfully applied to the programmable manipulation of 45 μm polystyrene beads; more than 100 particles were transported with an optical image-driven control, following the moving edge of the image at every moment. The effects of bead size and bias voltage on the manipulation speed were also investigated. Due to the portability and compatibility for disposable applications, this new platform has potential for programmable particle manipulation or chip-based bioprocessing including cell separation and bead-based analysis.

Keywords

Microfluidics Lab-on-a-display Liquid crystal display (LCD) Optoelectronic tweezers Dielectrophoresis Particle manipulation 

References

  1. Abe M, Orita M, Yamazaki H, Tsukamoto S, Teshima Y, Sakai T, Ohkubo T, Momozawa N, Sakai H (2004) Three-dimensional arrangements of polystyrene latex particles with a hyperbolic quadruple electrode system. Langmuir 20:5046–5051CrossRefGoogle Scholar
  2. Chiou PY, Ohta AT, Wu MC (2005) Massively parallel manipulation of single cells and microparticles using optical images. Nature 436:370–372CrossRefGoogle Scholar
  3. Choi S, Park J-K (2005) Microfluidic system for dielectrophoretic separation based on a trapezoidal electrode array. Lab Chip 5:1161–1167CrossRefGoogle Scholar
  4. Dittrich PS, Manz A (2006) Lab-on-a-Chip: microfluidics in drug discovery. Nat Rev Drug Discov 5:210–218CrossRefGoogle Scholar
  5. Doh I, Cho Y-H (2005) A continuous cell separation chip using hydrodynamic dielectrophoresis (DEP) process. Sens Actuators A, Phys 121:59–65CrossRefGoogle Scholar
  6. Hughes MP (2002) Strategies for dielectrophoretic separation in laboratory-on-a-chip systems. Electrophoresis 23:2569–2582CrossRefGoogle Scholar
  7. Krupke R, Hennrich F, Löhneysen Hv, Kappes MM (2003) Separation of metallic from semiconducting single-walled carbon nanotubes. Science 301:344–347CrossRefGoogle Scholar
  8. Lagally ET, Lee S-H, Soh HT (2005) Integrated microsystem for dielectrophoretic cell concentration and genetic detection. Lab Chip 5:1053–1058CrossRefGoogle Scholar
  9. Lao AIK, Hsing I-M (2005) Flow-based and sieving matrix-free DNA differentiation by a miniaturized field flow fractionation device. Lab Chip 5:687–690CrossRefGoogle Scholar
  10. Li H, Bashir R (2002) Dielectrophoretic separation and manipulation of live and heat-treated cells of Listeria on microfabricated devices with intedigitated electrodes. Sens Actuators B, Chem 86:215–221CrossRefGoogle Scholar
  11. Lu Y-S, Huang Y-P, Yeh AJ, Lee C, Chang Y-H (2005) Controllability of non-contact cell manipulation by image dielectrophoresis (iDEP). Opt Quantum Electron 37:1385–1395CrossRefGoogle Scholar
  12. Manaresi N, Romani A, Medoro G, Altomare L, Leonardi A, Tartagni M, Guerrieri R (2003) A CMOS chip for individual cell manipulation and detection. IEEE J Solid-St Circ 38:2297–2305CrossRefGoogle Scholar
  13. Minerick AR, Zhou R, Takhistov P, Chang H-C (2003) Manipulation and characterization of red blood cells with alternating current fields in microdevices. Electrophoresis 24:703–3717CrossRefGoogle Scholar
  14. Perch-Nielsen IR, Bang DD, Poulsen CR, El-Ali J, Wolff A (2003) Removal of PCR inhibitors using dielectrophoresis as a selective filter in a microsystem. Lab Chip 3:212–216CrossRefGoogle Scholar
  15. Ryu JI, Won SH, Hur JH, Kim HJ, Jang J, Jang GJ, Lee CW, Jung ST, Moon BY (2001) A novel amorphous silicon photoconductor array. J Korean Phys Soc 39:S264-S267Google Scholar
  16. Verpoorte E (2003) Beads and chips: new recipes for analysis. Lab Chip 3:60N–68NCrossRefGoogle Scholar
  17. Wang X-B, Yang J, Huang Y, Vykoukal J, Becker FF, Gascoyne PRC (2000) Cell separation by dielectrophoretic field-flow-fractionation. Anal Chem 72:832–839CrossRefGoogle Scholar
  18. Washizu M, Suzuki S, Kurosawa O, Nishizaka T, Shinohara T (1994) Molecular dielectrophoresis of biopolymers. IEEE T Ind Appl 30:835–843CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Wonjae Choi
    • 1
  • Se-Hwan Kim
    • 2
  • Jin Jang
    • 2
  • Je-Kyun Park
    • 1
  1. 1.Department of BioSystemsKorea Advanced Institute of Science and Technology (KAIST)DaejeonRepublic of Korea
  2. 2.Department of Information DisplayKyung Hee UniversitySeoulRepublic of Korea

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