Journal of Medical Ultrasonics

, Volume 41, Issue 4, pp 431–437 | Cite as

In vitro experiment using porcine artery for evaluation of ultrasonic measurement of arterial luminal surface profile

  • Yoshifumi Nagai
  • Magnus Cinthio
  • Hideyuki Hasegawa
  • Martin Bengtsson
  • Mikael Evander
  • John Albinsson
  • Hiroshi KanaiEmail author
Original Article



In early-stage atherosclerosis, the luminal surface of the arterial wall becomes rough because of detachment of endothelial cells and degeneration of the internal elastic layer. Therefore, it would be useful if minute luminal surface roughness of the carotid arterial wall, which occurs in the early stage of atherosclerosis, could be measured noninvasively with ultrasound. The injured luminal surface is believed to have roughness of a few hundred micrometers. However, in conventional ultrasonography, the axial resolution of a B-mode image depends on the ultrasonic wavelength (150 μm at ultrasonic center frequency of 10 MHz) because a B-mode image is constructed using the amplitude of the RF echo signal. Therefore, such surface roughness cannot be measured accurately from a conventional B-mode image. Recently, we successfully measured such minute surface profile transcutaneously using the phase shift of an ultrasonic echo from the carotid arterial wall. In our previous validation experiment, a silicone phantom with minute surface roughness of 10–20 μm was measured. However, the feasibility of our proposed method has never been validated using biological tissues.

Materials and methods

In the present study, luminal surface roughness of a porcine artery was measured and the result was evaluated by comparing it with the result measured using a stylus profilometer.

Results and conclusion

The root mean squared difference between the surface roughness measured by ultrasound and the stylus profilometer was 10.5 μm. This result proves that our proposed method can be used to measure minute surface roughness of biological tissue.


Luminal surface of arterial wall Roughness Porcine artery Atherosclerosis 


Conflict of interest


Ethical standard

In this study, no laboratory animals were used; only an excised porcine artery obtained from slaughterhouse waste was used.


  1. 1.
    Ikeshita K, Hasegawa H, Kanai H. Ultrasonic measurement of transient change in the stress-strain property of radial arterial wall caused by endothelial-dependent vasodilation. Jpn J Appl Phys. 2008;47:4165–7.CrossRefGoogle Scholar
  2. 2.
    Ikeshita K, Hasegawa H, Kanai H. Flow-mediated change in viscoelastic property of radial arterial wall measured by 22 MHz ultrasound. Jpn J Appl Phys. 2009;48:07GJ10-1–5.Google Scholar
  3. 3.
    Persson J, Formgren J, Israelsson B, Berglund G. Ultrasound-determined intima-media thickness and atherosclerosis. Arterioscler Thromb Vasc Biol. 1994;14:261–4.Google Scholar
  4. 4.
    Celermajer DS, Sorensen KE, Gooch VM, Spiegelhalter DJ, Miller OI, Sullivan ID, Lloyd JK, Deanfield JE. Non-invasive detection of endothelial dysfunction in children and adults at risk of atherosclerosis. Lancet. 1992;340:1111–5.PubMedCrossRefGoogle Scholar
  5. 5.
    Eiketsu S, Mien S, Tej MS, Nanjo H, Komatu M, Chengpei X, Masuda H, Christpher KZ. Arterial enlargement in response to high flow requires early expression of matrix metalloproteinases to degrade extracellular matrix. Exp Mol Pathol. 2002;73:142–53.CrossRefGoogle Scholar
  6. 6.
    Russell R. Atherosclerosis—an inflammatory disease. N Engl J Med. 1999;340:115–26.CrossRefGoogle Scholar
  7. 7.
    Schmidt-Trucksäss A, Sandrock M, Cheng D, Müller HM, Baumstark MW, Rauramaa R, Berg A, Huonker M. Quantitative measurement of carotid intima-media roughness—effect of age and manifest coronary artery disease. Atherosclerosis. 2003;166:57–65.PubMedCrossRefGoogle Scholar
  8. 8.
    Sandrock M, Schulze C, Schmitz D, Dickhuth H–H, Schmidt-Trucksäss A. Physical activity throughout life reduces the atherosclerotic wall process in the carotid artery. Br J Sports Med. 2008;42:839.PubMedCrossRefGoogle Scholar
  9. 9.
    Lili N, Ming Q, Wei Y, Long M, Yang X, Kelvin KLW, Derek A, Xin L, Hairong Z. Surface roughness detection of arteries via texture analysis of ultrasound images for early diagnosis of atherosclerosis. PLoS ONE. 2013;8:1–6.Google Scholar
  10. 10.
    Arihara C, Hasegawa H, Kanai H. Accurate ultrasonic measurement of surface profile using phase shift of echo and inverse filtering. Jpn J Appl Phys. 2006;45:4727–31.CrossRefGoogle Scholar
  11. 11.
    Cinthio M, Ahlgren ÅR, Jansson T, Eriksson A, Persson HW, Lindström K. Evaluation of an ultrasonic echo-tracking method for measurements of arterial wall movements in two dimensions. IEEE Trans Ultrason Ferroelectr Freq Control. 2005;52:1300–11.PubMedCrossRefGoogle Scholar
  12. 12.
    Cinthio M, Hasegawa H, Kanai H. Initial phantom validation of minute roughness measurement using phase tracking for arterial wall diagnosis non-invasively in vivo. IEEE Trans Ultrason Ferroelectr Freq Control. 2011;58:853–7.Google Scholar
  13. 13.
    Cinthio M, Hasegawa H, Kanai H. 11C-2 minute roughness measurement using phase tracking for arterial wall diagnosis non-invasively in vivo. IEEE Ultrasonics Symposium, New York. 2007. pp. 997–1000.Google Scholar
  14. 14.
    Kitamura K, Hasegawa H, Kanai H. Accurate estimation of carotid luminal surface roughness using ultrasonic radio-frequency echo. Jpn J Appl Phys. 2012; 51:07GF08-1–10.Google Scholar
  15. 15.
    Kanai H, Sato M, Koiwa Y, Chubachi N. Transcutaneous measurement and spectrum analysis of heart wall vibrations. IEEE Trans Ultrason Ferroelectr Freq Control. 1996; 43:791–10.Google Scholar
  16. 16.
    Kanai H, Sugimura K, Koiwa Y, Tsukahara Y. Accuracy evaluation in ultrasonic-based measurement of microscopic change in thickness. Electron Lett. 1999;35:949–50.CrossRefGoogle Scholar
  17. 17.
    Kanai H, Koiwa Y, Jianping Z. Real-time measurements of local myocardium motion and arterial wall thickening. IEEE Trans Ultrason Ferroelectr Freq Control. 1999;46:1229–41.PubMedCrossRefGoogle Scholar
  18. 18.
    Spyretta G, Antonio S, M. John L, Anil AB, Surinder D, Andrew NN. Carotid artery wall motion estimated from b-mode ultrasound using region tracking and block matching. Ultrasound Med. Biol. 2003; 29:387–9.Google Scholar
  19. 19.
    Honjo Y, Hasegawa H, Kanai H. Two-dimensional tracking of heart wall for detailed analysis of heart function at high temporal and spatial resolutions. Jpn J Appl Phys. 2010; 49:07HF14-1–9.Google Scholar

Copyright information

© The Japan Society of Ultrasonics in Medicine 2014

Authors and Affiliations

  • Yoshifumi Nagai
    • 1
  • Magnus Cinthio
    • 3
  • Hideyuki Hasegawa
    • 1
    • 2
  • Martin Bengtsson
    • 3
  • Mikael Evander
    • 3
  • John Albinsson
    • 3
  • Hiroshi Kanai
    • 1
    • 2
    Email author
  1. 1.Graduate School of Biomedical EngineeringTohoku UniversitySendaiJapan
  2. 2.Graduate School of EngineeringTohoku UniversitySendaiJapan
  3. 3.Department of Biomedical Engineering, Faculty of EngineeringLund UniversityLundSweden

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