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Journal of Medical Ultrasonics

, Volume 41, Issue 4, pp 431–437 | Cite as

In vitro experiment using porcine artery for evaluation of ultrasonic measurement of arterial luminal surface profile

  • Yoshifumi Nagai
  • Magnus Cinthio
  • Hideyuki Hasegawa
  • Martin Bengtsson
  • Mikael Evander
  • John Albinsson
  • Hiroshi KanaiEmail author
Original Article

Abstract

Introduction

In early-stage atherosclerosis, the luminal surface of the arterial wall becomes rough because of detachment of endothelial cells and degeneration of the internal elastic layer. Therefore, it would be useful if minute luminal surface roughness of the carotid arterial wall, which occurs in the early stage of atherosclerosis, could be measured noninvasively with ultrasound. The injured luminal surface is believed to have roughness of a few hundred micrometers. However, in conventional ultrasonography, the axial resolution of a B-mode image depends on the ultrasonic wavelength (150 μm at ultrasonic center frequency of 10 MHz) because a B-mode image is constructed using the amplitude of the RF echo signal. Therefore, such surface roughness cannot be measured accurately from a conventional B-mode image. Recently, we successfully measured such minute surface profile transcutaneously using the phase shift of an ultrasonic echo from the carotid arterial wall. In our previous validation experiment, a silicone phantom with minute surface roughness of 10–20 μm was measured. However, the feasibility of our proposed method has never been validated using biological tissues.

Materials and methods

In the present study, luminal surface roughness of a porcine artery was measured and the result was evaluated by comparing it with the result measured using a stylus profilometer.

Results and conclusion

The root mean squared difference between the surface roughness measured by ultrasound and the stylus profilometer was 10.5 μm. This result proves that our proposed method can be used to measure minute surface roughness of biological tissue.

Keywords

Luminal surface of arterial wall Roughness Porcine artery Atherosclerosis 

Notes

Conflict of interest

None.

Ethical standard

In this study, no laboratory animals were used; only an excised porcine artery obtained from slaughterhouse waste was used.

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Copyright information

© The Japan Society of Ultrasonics in Medicine 2014

Authors and Affiliations

  • Yoshifumi Nagai
    • 1
  • Magnus Cinthio
    • 3
  • Hideyuki Hasegawa
    • 1
    • 2
  • Martin Bengtsson
    • 3
  • Mikael Evander
    • 3
  • John Albinsson
    • 3
  • Hiroshi Kanai
    • 1
    • 2
    Email author
  1. 1.Graduate School of Biomedical EngineeringTohoku UniversitySendaiJapan
  2. 2.Graduate School of EngineeringTohoku UniversitySendaiJapan
  3. 3.Department of Biomedical Engineering, Faculty of EngineeringLund UniversityLundSweden

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