In vitro experiment using porcine artery for evaluation of ultrasonic measurement of arterial luminal surface profile
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In early-stage atherosclerosis, the luminal surface of the arterial wall becomes rough because of detachment of endothelial cells and degeneration of the internal elastic layer. Therefore, it would be useful if minute luminal surface roughness of the carotid arterial wall, which occurs in the early stage of atherosclerosis, could be measured noninvasively with ultrasound. The injured luminal surface is believed to have roughness of a few hundred micrometers. However, in conventional ultrasonography, the axial resolution of a B-mode image depends on the ultrasonic wavelength (150 μm at ultrasonic center frequency of 10 MHz) because a B-mode image is constructed using the amplitude of the RF echo signal. Therefore, such surface roughness cannot be measured accurately from a conventional B-mode image. Recently, we successfully measured such minute surface profile transcutaneously using the phase shift of an ultrasonic echo from the carotid arterial wall. In our previous validation experiment, a silicone phantom with minute surface roughness of 10–20 μm was measured. However, the feasibility of our proposed method has never been validated using biological tissues.
Materials and methods
In the present study, luminal surface roughness of a porcine artery was measured and the result was evaluated by comparing it with the result measured using a stylus profilometer.
Results and conclusion
The root mean squared difference between the surface roughness measured by ultrasound and the stylus profilometer was 10.5 μm. This result proves that our proposed method can be used to measure minute surface roughness of biological tissue.
KeywordsLuminal surface of arterial wall Roughness Porcine artery Atherosclerosis
Conflict of interest
In this study, no laboratory animals were used; only an excised porcine artery obtained from slaughterhouse waste was used.
- 2.Ikeshita K, Hasegawa H, Kanai H. Flow-mediated change in viscoelastic property of radial arterial wall measured by 22 MHz ultrasound. Jpn J Appl Phys. 2009;48:07GJ10-1–5.Google Scholar
- 3.Persson J, Formgren J, Israelsson B, Berglund G. Ultrasound-determined intima-media thickness and atherosclerosis. Arterioscler Thromb Vasc Biol. 1994;14:261–4.Google Scholar
- 9.Lili N, Ming Q, Wei Y, Long M, Yang X, Kelvin KLW, Derek A, Xin L, Hairong Z. Surface roughness detection of arteries via texture analysis of ultrasound images for early diagnosis of atherosclerosis. PLoS ONE. 2013;8:1–6.Google Scholar
- 12.Cinthio M, Hasegawa H, Kanai H. Initial phantom validation of minute roughness measurement using phase tracking for arterial wall diagnosis non-invasively in vivo. IEEE Trans Ultrason Ferroelectr Freq Control. 2011;58:853–7.Google Scholar
- 13.Cinthio M, Hasegawa H, Kanai H. 11C-2 minute roughness measurement using phase tracking for arterial wall diagnosis non-invasively in vivo. IEEE Ultrasonics Symposium, New York. 2007. pp. 997–1000.Google Scholar
- 14.Kitamura K, Hasegawa H, Kanai H. Accurate estimation of carotid luminal surface roughness using ultrasonic radio-frequency echo. Jpn J Appl Phys. 2012; 51:07GF08-1–10.Google Scholar
- 15.Kanai H, Sato M, Koiwa Y, Chubachi N. Transcutaneous measurement and spectrum analysis of heart wall vibrations. IEEE Trans Ultrason Ferroelectr Freq Control. 1996; 43:791–10.Google Scholar
- 18.Spyretta G, Antonio S, M. John L, Anil AB, Surinder D, Andrew NN. Carotid artery wall motion estimated from b-mode ultrasound using region tracking and block matching. Ultrasound Med. Biol. 2003; 29:387–9.Google Scholar
- 19.Honjo Y, Hasegawa H, Kanai H. Two-dimensional tracking of heart wall for detailed analysis of heart function at high temporal and spatial resolutions. Jpn J Appl Phys. 2010; 49:07HF14-1–9.Google Scholar