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Journal of Medical Ultrasonics

, Volume 41, Issue 1, pp 11–21 | Cite as

Ultrasound image-guided therapy enhances antitumor effect of cisplatin

  • Noboru Sasaki
  • Nobuki Kudo
  • Kensuke Nakamura
  • Sue Yee Lim
  • Masahiro Murakami
  • W. R. Bandula Kumara
  • Yu Tamura
  • Hiroshi Ohta
  • Masahiro Yamasaki
  • Mitsuyoshi TakiguchiEmail author
Original Article

Abstract

Purpose

The aim of this study was to clarify whether ultrasound image-guided cisplatin delivery with an intratumor microbubble injection enhances the antitumor effect in a xenograft mouse model.

Methods

Canine thyroid adenocarcinoma cells were used for all experiments. Before in vivo experiments, the cisplatin and microbubble concentration and ultrasound exposure time were optimized in vitro. For in vivo experiments, cells were implanted into the back of nude mice. Observed by a diagnostic ultrasound machine, a mixture of cisplatin and ultrasound contrast agent, Sonazoid, microbubbles was injected directly into tumors. The amount of injected cisplatin and microbubbles was 1 μg/tumor and 1.2 × 107 microbubbles/tumor, respectively, with a total injected volume of 20 μl. Using the same diagnostic machine, tumors were exposed to ultrasound for 15 s. The treatment was repeated four times.

Results

The combination of cisplatin, microbubbles, and ultrasound significantly delayed tumor growth as compared with no treatment (after 18 days, 157 ± 55 vs. 398 ± 49 mm3, P = 0.049). Neither cisplatin alone nor the combination of cisplatin and ultrasound delayed tumor growth. The treatment did not decrease the body weight of mice.

Conclusion

Ultrasound image-guided anticancer drug delivery may enhance the antitumor effects of drugs without obvious side effects.

Keywords

Diagnostic ultrasound Microbubble Image-guided drug delivery 

Notes

Acknowledgments

This study was partly supported by a Grant-in-Aid for Scientific Research (No. 23580436) from the Japan Society for the Promotion of Science (M.T.). The authors would like to thank Miss Nao Watanabe for her help in in vivo experiments.

Conflict of interest

None.

Supplementary material

Supplementary material 1 (MPG 19074 kb)

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Copyright information

© The Japan Society of Ultrasonics in Medicine 2013

Authors and Affiliations

  • Noboru Sasaki
    • 1
  • Nobuki Kudo
    • 2
  • Kensuke Nakamura
    • 3
  • Sue Yee Lim
    • 1
  • Masahiro Murakami
    • 1
  • W. R. Bandula Kumara
    • 1
  • Yu Tamura
    • 1
  • Hiroshi Ohta
    • 1
  • Masahiro Yamasaki
    • 1
  • Mitsuyoshi Takiguchi
    • 1
    Email author
  1. 1.Laboratory of Veterinary Internal Medicine, Graduate School of Veterinary MedicineHokkaido UniversitySapporoJapan
  2. 2.Laboratory of Biomedical Engineering, Graduate School of Information Science and TechnologyHokkaido UniversitySapporoJapan
  3. 3.Veterinary Teaching Hospital, Graduate School of Veterinary MedicineHokkaido UniversitySapporoJapan

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