EcoHealth

, Volume 11, Issue 2, pp 207–214

Attempted Detection of Toxoplasma gondii Oocysts in Environmental Waters Using a Simple Approach to Evaluate the Potential for Waterborne Transmission in the Galápagos Islands, Ecuador

  • Michelle L. Verant
  • Noemi d’Ozouville
  • Patricia G. Parker
  • Karen Shapiro
  • Elizabeth VanWormer
  • Sharon L. Deem
Short Communication

DOI: 10.1007/s10393-013-0888-5

Cite this article as:
Verant, M.L., d’Ozouville, N., Parker, P.G. et al. EcoHealth (2014) 11: 207. doi:10.1007/s10393-013-0888-5

Abstract

Toxoplasmosis is a health concern for wildlife and humans, particularly in island ecosystems. In the Galápagos Islands, exposure to Toxoplasma gondii has been found in marine avifauna on islands with and without domestic cats. To evaluate potential waterborne transmission of T. gondii, we attempted to use filtration and epifluorescent microscopy to detect autofluorescent T. gondii oocysts in fresh and estuarine surface water samples. T. gondii oocyst-like structures were microscopically visualized but were not confirmed by polymerase chain reaction and sequence analyses. Further research is needed to refine environmental pathogen screening techniques and to evaluate disease risk of waterborne zoonoses such as T. gondii for wildlife and humans, particularly in the Galápagos and other naive island ecosystems.

Keywords

Toxoplasma gondii waterborne zoonosis Galápagos epifluorescent microscopy 

Supplementary material

10393_2013_888_MOESM1_ESM.docx (17 kb)
Supplementary material 1 (DOCX 16 kb)

Copyright information

© International Association for Ecology and Health 2013

Authors and Affiliations

  • Michelle L. Verant
    • 1
  • Noemi d’Ozouville
    • 2
    • 3
  • Patricia G. Parker
    • 3
    • 4
    • 5
  • Karen Shapiro
    • 6
  • Elizabeth VanWormer
    • 6
  • Sharon L. Deem
    • 3
    • 4
    • 7
  1. 1.University of Wisconsin-Madison, School of Veterinary MedicineMadisonUSA
  2. 2.Galapagos Islands Integrated Water StudiesUPMC-Sorbonne Universités & CNRSSisypheFrance
  3. 3.Charles Darwin FoundationSanta CruzEcuador
  4. 4.University of Missouri–St. LouisSt. LouisUSA
  5. 5.WildCare InstituteSaint Louis ZooSt. LouisUSA
  6. 6.University of California-Davis, School of Veterinary Medicine, One Health InstituteDavisUSA
  7. 7.Institute for Conservation MedicineSaint Louis ZooSt. LouisUSA

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