EcoHealth

, Volume 8, Issue 4, pp 507–511 | Cite as

High Numbers of Vibrio vulnificus in Tar Balls Collected from Oiled Areas of the North-Central Gulf of Mexico Following the 2010 BP Deepwater Horizon Oil Spill

Short Communication

Abstract

The Deepwater Horizon Oil Spill was the largest oil spill in USA history releasing approximately 4.9 million barrels of crude oil into the Gulf of Mexico. Soon after the spill started, tar balls and other forms of weathered oil appeared in large numbers on beaches in Mississippi and Alabama. In this study, we analyzed tar balls for total aerobic bacterial (TAB) counts and also for the presence of Vibrio vulnificus, a human pathogen known to be abundant in the Gulf Coast environment and capable of causing severe wound infections by contact with contaminated surfaces. Our results showed that TAB counts were significantly higher in tar balls than in sand and seawater collected at the same location. In addition, V. vulnificus numbers were 10× higher in tar balls than in sand and up to 100× higher than in seawater. Densities of V. vulnificus were higher than 105 colony forming units/g of tar ball in all samples analyzed. Our data suggest that tar balls can act as reservoirs for bacteria including human pathogens.

Keywords

Vibrio vulnificus Tar balls Deepwater Horizon oil spill 

References

  1. Depaola A, Capers G M, Alexander D (1994) Densities of Vibrio vulnificus in the intestines of fish from the U.S. Gulf Coast. Applied and Environmental Microbiology 60:984–988.PubMedGoogle Scholar
  2. FDA (2002) MPN procedure for the enumeration of Vibrio vulnificus using gene probe for identification. http://Www.Fda.Gov/Food/Scienceresearch/Laboratorymethods/Bacteriologicalanalyticalmanualbam/Ucm072659.Htm
  3. Federal Interagency Solutions Group (FISG) (2010) Oil budget calculator: Deepwater Horizon. http://Www.Restorethegulf.Gov/Sites/Default/Files/Documents/Pdf/Oilbudgetcalc_Full_Hq-Print_111110.Pdf
  4. Goodman R (2003) Tar balls: the end state. Spill Science & Technology Bulletin 8:117–121.CrossRefGoogle Scholar
  5. Hazen T C, Dubinsky E A, Desantis T Z, Andersen G L, Piceno Y M, Singh N, Jansson J K, Probst A, Borglin S E, Fortney J L, Stringfellow W T, Bill M, Conrad M E, Tom L M, Chavarria K L, Alusi T R, Lamendella R, Joyner D C, Spier C, Baelum J, Auer M, Zelma M L, Chakraborty R, Sonnenthal E L, D’haeseleer P, Holman N, Osman S, Lu Z, Van Nostrand J, Deng Y, Zhou J, Mason O U (2010) Deep-sea oil plume enriches indigenous oil-degrading bacteria. Science 330:204–208.PubMedCrossRefGoogle Scholar
  6. Itah a Y, Essien J P (2005) Growth profile and hydrocarbonoclastic potential of microorganisms isolated from tarballs in the Bight of Bonny, Nigeria. World Journal of Microbiology & Biotechnology 21:1317-1322.CrossRefGoogle Scholar
  7. Mead P S, Slutsker L, Dietz V, Mcgaig L F, Bressee J S, Shapiro C, Griffin P M, Tauxe R V (1999) Food-related illness and death in the United States. Emergent Infectious Diseases 5:607–625.CrossRefGoogle Scholar
  8. Motes M L, Depaola A, Cook D W, Veazey J E, Hunsucker J C, Garthright W E, Blodgett R J, Chirtel S J (1998) Influence of water temperature and salinity on Vibrio vulnificus in Northern Gulf and Atlantic Coast oysters (Crassostrea virginica). Applied and Environmental Microbiology 64:1459–1465.PubMedGoogle Scholar
  9. NOAA (2000) Shoreline assessment manual (version 3.0). Hazardous Materials Response Division, National Oceanic and Atmospheric Administration, Seattle, WA.Google Scholar
  10. NOAA (2010) Understanding tar balls. NOAA’s oil spill response.Google Scholar
  11. Strom M S, Paranjpye R N (2000) Epidemiology and pathogenesis of Vibrio vulnificus. Microbes and infection 2:177–188.PubMedCrossRefGoogle Scholar
  12. Thompson F L, Iida T, Swings J (2004) Biodiversity of Vibrios. Microbiology and Molecular Biology Reviews 68:403–431.PubMedCrossRefGoogle Scholar
  13. Voosen P (2010) Will bacterial plague follow crude oil spill along Gulf coast? In: The New York Times, New York: Arthur Ochs Suizberger, Jr.Google Scholar
  14. Wright A C, Miceli G A, Landry W L, Christy J B, Watkins W D, Morris J G (1993) Rapid identification of Vibrio vulnificus on nonselective media with an alkaline phosphatase-labeled oligonucleotide probe. Applied and Environmental Microbiology 59:541–546.PubMedGoogle Scholar

Copyright information

© International Association for Ecology and Health 2011

Authors and Affiliations

  1. 1.Aquatic Microbiology LaboratoryDepartment of Fisheries and Allied Aquacultures, Auburn UniversityAuburnUSA
  2. 2.Aquatic Parasitology LaboratoryDepartment of Fisheries and Allied Aquacultures, Auburn UniversityAuburnUSA

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