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EcoHealth

, Volume 4, Issue 3, pp 338–345 | Cite as

The Immune Response of the Tasmanian Devil (Sarcophilus harrisii) and Devil Facial Tumour Disease

  • Gregory M. Woods
  • Alexandre Kreiss
  • Katherine Belov
  • Hannah V. Siddle
  • David L. Obendorf
  • H. Konrad Muller
Special Focus: Tasmanian Devil Declines

Abstract

One of the most remarkable aspects of Devil Facial Tumour Disease (DFTD) is its infectious nature, and for successful transmission it must avoid detection by the devil’s immune system. For this to occur, the devil either is severely immunosuppressed or factors produced by the tumor contribute to its avoidance of immune detection. An analysis of the devil’s immune system revealed the presence of normal-looking lymphoid organs and lymphoid cells. At a functional level the lymphocytes proliferated in response to mitogen stimulation. Subcutaneous injection of a cellular antigen produced a strong antibody response, providing compelling evidence that the devil has a competent immune system. Tumor cell analysis demonstrated that the tumor expresses the genes of the major histocompatibility complex; however, there was a limited diversity. Therefore, the most likely explanation for devil-to-devil transmission of DFTD is that the tumor is not recognized by the devil as “non-self” because of the limited genetic diversity. With its consistent morphology and relatively stable genome, this tumor would provide a reasonable target for a vaccine approach, provided the immune system can be coaxed into recognizing the tumor as “non-self.”

Keywords

Devil Facial Tumour Disease immune response vaccine lymphoid tissue major histocompatibility complex lymphocyte proliferation 

Notes

Acknowledgments

The authors gratefully acknowledge all members of the Devil Facial Tumour Disease Team. They also thank Dr. Barrie Wells and Dr. James Harris for veterinary assistance and Candice Clark for technical assistance. They also acknowledge the assistance provided by the Pathology Department, Royal Hobart Hospital. Financial support for this work came from the Department of Primary Industry and Water and research grants from the Eric Guiler Foundation, the Australian Research Council, and the Sarah and Anne Payten Canine Cancer Fund. Ethics was approved by the Animal Ethics Committee of Tasmanian’s Park and Wildlife Services, permit numbers 33/2004–5 and 32/2005–6

References

  1. Ada G (2005) Overview of vaccines and vaccination. Molecular Biotechnology 29:255–272CrossRefGoogle Scholar
  2. Algarra I, Cabrera T, Garrido F (2000) The HLA crossroad in tumor immunology. Human Immunology 61:65–73CrossRefGoogle Scholar
  3. Belov K, Deakin JE, Papenfuss AT, Baker ML, Melman SD, Siddle HV, et al. (2006) Reconstructing an ancestral mammalian immune supercomplex from a marsupial major histocompatibility complex. PLoS Biology 4:e46; DOI:10.1371/journal.pbio.0040046CrossRefGoogle Scholar
  4. Canfield PJ, Hemsley S (2000) The roles of histology and immunohistology in the investigation of marsupial disease and normal lymphoid tissue. Developmental and Comparative Immunology 24:455–471CrossRefGoogle Scholar
  5. Croix DA, Samples NK, Vandeberg JL, Stone WH (1989) Immune response of a marsupial (Monodelphis domestica) to sheep red blood cells. Developmental and Comparative Immunology 13:73–78CrossRefGoogle Scholar
  6. Das U, Das AK (2000) Review of canine transmissible venereal sarcoma. Veterinary Research Communications 24:545–556CrossRefGoogle Scholar
  7. Haydon DT, Randall DA, Matthews L, Knobel DL, Tallents LA, Gravenor MB, Williams SD, Pollinger JP, Cleaveland S, Woolhouse ME, Sillero-Zubiri C, Marino J, Macdonald DW, Laurenson MK (2006) Low-coverage vaccination strategies for the conservation of endangered species. Nature 443:692–695CrossRefGoogle Scholar
  8. Jones ME, Paetkau D, Geffen E, Moritz C (2004) Genetic diversity and population structure of Tasmanian devils, the largest marsupial carnivore. Molecular Ecology 13:2197–2209CrossRefGoogle Scholar
  9. Loh R, Bergfeld J, Hayes D, O’Hara A, Pyecroft S, Raidal S, Sharpe R (2006a) The pathology of devil facial tumor disease (DFTD) in Tasmanian Devils (Sarcophilus harrisii). Veterinary Pathology 43:890–895Google Scholar
  10. Loh R, Hayes D, Mahjoor A, O’Hara A, Pyecroft S, Raidal S (2006b) The immunohistochemical characterization of devil facial tumor disease (DFTD) in the Tasmanian Devil (Sarcophilus harrisii). Veterinary Pathology 43:896–903Google Scholar
  11. Menotti-Raymond MA, O’Brien SJ (1995) Evolutionary conservation of ten microsatellite loci in four species of Felidae. Journal of Heredity 86:319–322Google Scholar
  12. Montali RJ, Bush M, Cromie R, Holland SM, Maslow JN, Worley M, Witebsky FG, Phillips TM (1998) Primary Mycobacterium avium complex infections correlate with lowered cellular immune reactivity in Matschie’s tree kangaroos (Dendrolagus matschiei). Journal of Infectious Diseases 178:1719–1725CrossRefGoogle Scholar
  13. Murgia C, Pritchard JK, Kim SY, Fassati A, Weiss RA (2006) Clonal origin and evolution of a transmissible cancer. Cell 126:477–487CrossRefGoogle Scholar
  14. Pearse AM, Swift K (2006) Allograft theory: transmission of devil facial-tumour disease. Nature 439:549CrossRefGoogle Scholar
  15. Rosenberg SA (2004) Development of effective immunotherapy for the treatment of patients with cancer. Journal of the American College of Surgeons 198:685–696CrossRefGoogle Scholar
  16. Stone WH, Brunn DA, Foster EB, Manis GS, Hoffman ES, Saphire DG, VandenBerg JL, Infante AJ (1998) Absence of a significant mixed lymphocyte reaction in a marsupial (Monodelphis domestica). Laboratory Animal Science 48:184–189Google Scholar
  17. Wilkinson R, Kotlarski I, Barton M, Phillips P (1992) Isolation of koala lymphoid cells and their in vitro responses to mitogens. Veterinary Immunology and Immunopathology 31:21–33CrossRefGoogle Scholar
  18. Young LJ, Deane EM (2007) Culture and stimulation of tammar wallaby lymphocytes. Veterinary Research Communications 31:685–701CrossRefGoogle Scholar

Copyright information

© Ecohealth Journal Consortium 2007

Authors and Affiliations

  • Gregory M. Woods
    • 1
  • Alexandre Kreiss
    • 1
  • Katherine Belov
    • 2
  • Hannah V. Siddle
    • 2
  • David L. Obendorf
    • 3
  • H. Konrad Muller
    • 1
  1. 1.Menzies Research InstituteUniversity of TasmaniaTasmaniaAustralia
  2. 2.Faculty of Veterinary ScienceUniversity of SydneyNew South WalesAustralia
  3. 3.School of MedicineUniversity of TasmaniaTasmaniaAustralia

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