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Esophagus

, Volume 10, Issue 4, pp 193–198 | Cite as

Clathrin heavy chain is a useful immunohistochemical marker for esophageal squamous intraepithelial neoplasia

  • Kazuya Tokita
  • Masanori SeimiyaEmail author
  • Kazuyuki Matsushita
  • Takeshi Tomonaga
  • Kiyotaka Onodera
  • Syoji Ohki
  • Tohru Tanizawa
  • Masaya Uesato
  • Hideaki Shimada
  • Hisahiro Matsubara
  • Yukio Nakatani
  • Fumio Nomura
Original Article

Abstract

Background

Recent advances in the endoscopic diagnosis and treatment of esophageal cancer have facilitated the detection and treatment of minute tumors, necessitating the accurate histopathological diagnosis of early esophageal cancer or precancerous lesions. This study evaluated the usefulness of immunohistochemical analysis (IHC) of clathrin heavy chain (CHC) as a marker for early esophageal cancer.

Methods

The immunoreactivity of CHC was analyzed in 409 esophageal specimens using a tissue array. Immunoreactivities of CHC, p53, and Ki67 were then compared in 44 endoscopically resected specimens.

Results

CHC expression was significantly stronger in the cytoplasm of esophageal squamous cell carcinomas compared with non-tumor specimens in the tissue array. CHC expression in endoscopic specimens was significantly stronger in the cytoplasm of high-grade intraepithelial neoplasias and superficial carcinomas than in benign squamous epithelium and low-grade intraepithelial neoplasias. The sensitivity and specificity of CHC for the diagnosis of esophageal lesions were 75 and 96 %, respectively. These accuracies were comparable with those of p53 (43 and 98 %) and Ki67 (68 and 100 %). In addition, the sensitivity was increased by using a combination of markers as follows: 80 %, CHC + p53; 78 %, CHC + Ki67; 90 %, CHC + p53 + Ki67.

Conclusions

CHC detected by IHC may be a useful marker for the pathological diagnosis of esophageal squamous intraepithelial neoplasia.

Keywords

Clathrin heavy chain Endoscopy Esophageal cancer Immunohistochemistry Marker 

Notes

Acknowledgments

This study received financial support from a Grant-in-Aid for Scientific Research (C) from the Japan Society for the Promotion of Science (no. 22590518 and 25460675).

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical standard statement

The authors work conformed to the guidelines set forth in the Helsinki Declaration of 1975, as revised in 2000.

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Copyright information

© The Japan Esophageal Society and Springer Japan 2013

Authors and Affiliations

  • Kazuya Tokita
    • 1
    • 2
  • Masanori Seimiya
    • 2
    • 3
    Email author
  • Kazuyuki Matsushita
    • 2
    • 3
  • Takeshi Tomonaga
    • 4
  • Kiyotaka Onodera
    • 5
  • Syoji Ohki
    • 5
  • Tohru Tanizawa
    • 6
  • Masaya Uesato
    • 7
  • Hideaki Shimada
    • 8
  • Hisahiro Matsubara
    • 7
  • Yukio Nakatani
    • 5
    • 9
  • Fumio Nomura
    • 2
    • 3
  1. 1.Division of Laboratory MedicineNational Cancer Center Research InstituteTokyoJapan
  2. 2.Department of Molecular Diagnosis, Graduate School of MedicineChiba UniversityChibaJapan
  3. 3.Division of Laboratory MedicineChiba University HospitalChibaJapan
  4. 4.Laboratory of Proteome ResearchNational Institute of Biomedical InnovationOsakaJapan
  5. 5.Department of PathologyChiba University HospitalChibaJapan
  6. 6.Clinical LaboratoryTokyo Metropolitan Bokuto HospitalTokyoJapan
  7. 7.Department of Frontier Surgery, Graduate School of MedicineChiba UniversityChibaJapan
  8. 8.Department of SurgeryToho University School of MedicineTokyoJapan
  9. 9.Department of Diagnostic Pathology, Graduate School of MedicineChiba UniversityChibaJapan

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