Isolation, identification and culture of myenteric plexus cells from ovine esophagus
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The myenteric plexus of the esophagus consists of two major cell types, neurons and enteric glial cells, and functions to coordinate peristaltic waves. The aim of this study was to develop a protocol for the isolation of myenteric plexus and dissociation and culture of myenteric plexus cells.
The myenteric plexus was isolated from the ovine esophagus through treatment with collagenase, followed by dissociation of cells with trypsin/EDTA. Myenteric plexus cells were cultured in vitro and the different cell components were identified by immunohistochemical staining.
Isolated myenteric plexi expressed enteric glial cell markers S-100 and GFAP and enteric neuronal cell marker PGP 9.5. Furthermore, c-kit positive cells were also detected, which may represent the interstitial cells of Cajal. Despite the successful isolation of a wide range of myenteric plexus cells, dissociation of cells was poor and requires further optimization.
This study reports on the isolation and dissociation of the principle cells of the myenteric plexus from ovine esophagus. As the ovine model is a clinically relevant large animal model for esophageal disease, the isolation of ovine myenteric plexus cells is of significant importance for the understanding of pathologies of the enteric nervous system and application in gastrointestinal tissue engineering.
KeywordsEnteric nervous system GFAP PGP 9.5 Tissue engineering
This research is funded by the European Union within the 6th Framework Program (EuroSTEC; LSHC-CT-2006-037409).
Conflict of interest
The authors declare that they have no conflict of interest.
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