Japanese Journal of Ophthalmology

, Volume 62, Issue 5, pp 592–597 | Cite as

Perfluorocarbon liquid-assisted membrane staining and peeling technique for macular diseases

  • Yoshifumi OkamotoEmail author
  • Fumiki Okamoto
  • Tetsuro Oshika
Clinical Investigation



A surgical technique is described that facilitates membrane peeling in patients with macular diseases by using small amounts of perfluorocarbon liquid (PFCL) and brilliant blue G (BBG) dye.

Study Design

Retrospective cohort study.


After placing about 1.0-1.5 cc PFCL on the macular area, BBG was applied with a 27-gauge blunt needle at the interface of PFCL and retina. The membrane peeling was performed under PFCL.


The amount of BBG dye with PFCL-assisted staining technique has the potential to be smaller than in conventional staining method. Since the displacement angle of the membrane during peeling procedures is considerably increased by PFCL, combined with its high specific gravity and interfacial tension, the risk of tearing the membrane during manipulation is reduced to a great extent. The postoperative visual function in patients with PFCL-assisted peeling was comparable to that of patients without PFCL-assisted peeling.


The PFCL-assisted technique enables sufficient membrane staining with minimal amounts of BBG dye, with tearing of the epiretinal membrane or internal limiting membrane being less likely than with the conventional method.


Brilliant blue G Epiretinal membrane Inverted internal limiting membrane flap technique Macular hole Perfluorocarbon liquid 



Publication of this article was supported by the Department of Ophthalmology, Faculty of Medicine, University of Tsukuba, Ibaraki, Japan. Involved in design of study (Y.O., F.O., T.O.); conduct of study (Y.O., F.O.); data collection (Y.O.); management, analysis, and interpretation of the data (Y.O., F.O.); preparation of the manuscript (Y.O.); review of the manuscript (T.O.); and approval of the manuscript (Y.O., F.O., T.O.).

Conflicts of interest

Y Okamoto, None; F. Okamoto, None; T. Oshika, None.

Supplementary material

10384_2018_613_MOESM1_ESM.mp4 (18.9 mb)
Supplemental Digital Content 1. Video that demonstrates the procedure of membrane staining using a minimal amount of BBG dye. mp4 (MP4 19340 kb)
10384_2018_613_MOESM2_ESM.mp4 (10.8 mb)
Supplemental Digital Content 2. Video that demonstrates the procedure of the PFCL-assisted epiretinal membrane and ILM peeling technique which reduced risk of tearing. mp4 (MP4 11092 kb)


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Copyright information

© Japanese Ophthalmological Society 2018

Authors and Affiliations

  • Yoshifumi Okamoto
    • 1
    • 2
    Email author
  • Fumiki Okamoto
    • 1
  • Tetsuro Oshika
    • 1
  1. 1.Department of Ophthalmology, Faculty of MedicineUniversity of TsukubaTsukubaJapan
  2. 2.Department of OphthalmologyMito Kyodo General HospitalMitoJapan

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