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Japanese Journal of Ophthalmology

, Volume 61, Issue 5, pp 369–377 | Cite as

Pre-banking microbial contamination of donor conjunctiva and storage medium for penetrating keratoplasty

  • Takenori Inomata
  • Koichi Ono
  • Tsuyoshi Matsuba
  • Tina Shiang
  • Antonio Di Zazzo
  • Satoru Nakatani
  • Masahiro Yamaguchi
  • Nobuyuki Ebihara
  • Akira Murakami
Clinical Investigation

Abstract

Purpose

The aims of this study were to investigate the incidence of positive donor tissue cultures before transfer to preservation medium (Optisol™-GS) for penetrating keratoplasty, to verify the efficacy of antibiotics contained in Optisol™-GS by examining the drug susceptibility and to assess the relationship between the results of our microbial assessments as well as donor factors and the incidence of contamination.

Methods

We conducted a retrospective, cross-sectional study using Juntendo Eye Bank records for all corneal transplantations. Two hundred donor conjunctiva harvestings and storage medium (EP-II®) cultures were performed between July 2008 and June 2011. We analyzed the associations between donor factors (age, gender, history of cataract surgery, death-to-preservation interval, cause of death) and contamination rates using multivariate analysis by the generalized estimating equation model.

Results

We obtained positive bacterial cultures from 154 of the 200 eyes (77.0%). The isolated bacteria were indigenous, such as coagulase-negative Staphylococci, Corynebacterium sp., and methicillin-resistant Staphylococcus aureus (MRSA). There was significant resistance to levofloxacin (18 eyes, 9.0%) and gentamicin (12 eyes, 6.0%), and no vancomycin-resistant bacteria were detected. The donor factors did not correlate with the prevalence of bacterial contamination in our criteria.

Conclusions

Pre-banking microbial assessment allows for microbial detection, bacterial susceptibility and resistance testing. This is useful for developing preservation mediums containing effective spectrum antibiotic agents for high quality control of corneal banking.

Keywords

Eye banking Bacterial contamination Donor conjunctiva Corneoscleral rim Preservation medium 

Notes

Acknowledgements

We thank Juntendo Eye Bank, Japan Organ Transplant Network and Oyama Lions Club for their cornea donations. The authors thank Dr. Alireza Mashaghi, Dr. Jiaxu Hong and Dr. Masahiro Nakamura for critically reading and editing the manuscript, Dr. Ayako Nakamura for her microbiological insight and Peter Mallen for his assistance in creating the elegant figure.

Conflicts of interest

All authors declared that they have no conflict of interest.

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Copyright information

© Japanese Ophthalmological Society 2017

Authors and Affiliations

  • Takenori Inomata
    • 1
  • Koichi Ono
    • 2
  • Tsuyoshi Matsuba
    • 3
  • Tina Shiang
    • 4
  • Antonio Di Zazzo
    • 5
  • Satoru Nakatani
    • 1
  • Masahiro Yamaguchi
    • 1
  • Nobuyuki Ebihara
    • 6
  • Akira Murakami
    • 1
  1. 1.Department of OphthalmologyJuntendo University Faculty of MedicineTokyoJapan
  2. 2.Department of OphthalmologyJuntendo Tokyo Koto Geriatric Medical Center, Juntendo University School of MedicineTokyoJapan
  3. 3.Advanced Research Center for Human SciencesWaseda UniversityTokorozawaJapan
  4. 4.Department of Ophthalmology, Schepens Eye Research Institute, Massachusetts Eye and Ear InfirmaryHarvard Medical SchoolBostonUSA
  5. 5.BioMolecular and Cellular Laboratories in OphthalmologyIRCCS, G.B. Bietti FoundationRomeItaly
  6. 6.Department of OphthalmologyJuntendo University Urayasu HospitalTomiokaJapan

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