Japanese Journal of Ophthalmology

, Volume 55, Issue 5, pp 547–557 | Cite as

Efficacy of commercial soft contact lens disinfectant solutions against Acanthamoeba

  • Takeshi Kobayashi
  • Lindsay Gibbon
  • Tsuyoshi Mito
  • Atsushi Shiraishi
  • Toshihiko Uno
  • Yuichi Ohashi
Laboratory Investigation



To investigate the relative efficacy of Japanese commercial soft contact lens disinfectant solutions against Acanthamoeba trophozoites and cysts.

Materials and methods

Eight types of multipurpose solution (MPS), two types of hydrogen peroxide solution, and one povidone–iodine solution were evaluated to determine their effect against Acanthamoeba trophozoites and cysts (ATCC 50514). Acanthamoeba cysts were cultured in encystment medium for either 1 or 2 weeks (1 and 2-week-old cysts). The trophozoites and cysts were treated with each disinfectant solution for 0, 2, 4, 8, or 24 h. After performing four tenfold serial dilutions of each test solution, dilutions were cultured for 10 days. The number of surviving organisms was calculated using the trimmed Spearman–Karber method.


Among the MPS tested, only four were effective against trophozoites after treatment for 4 h, and none was effective against 2-week-old cysts. Hydrogen peroxide had a significant effect on trophozoites and 1-week-old cysts, but not on 2-week-old cysts. In contrast, povidone–iodine caused a 2.6 log reduction in 2-week-old cysts.


MPS were found to have limited efficacy against trophozoites and no efficacy against 2-week-old cysts. Only povidone–iodine had any efficacy against 2-week-old cysts.


Acanthamoeba Soft contact lenses (SCL) Disinfectant solution Multipurpose solution (MPS) Mature cysts 



This study was a collaborative effort made possible by assistance from the National Consumer Affairs Center of Japan and the Japan Contact Lens Society. The authors would like to thank Ai Onodera, Satoshi Fukuyama, Kazumi Hishida, Saori Sourin, and Tetsuo Yanagihashi of the National Consumer Affairs Center of Japan Product Testing Department for their contributions to this study. The authors would also like to thank Dr Yasuhisa Ishibashi for his critical suggestions.


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Copyright information

© Japanese Ophthalmological Society 2011

Authors and Affiliations

  • Takeshi Kobayashi
    • 1
  • Lindsay Gibbon
    • 2
  • Tsuyoshi Mito
    • 2
  • Atsushi Shiraishi
    • 1
    • 3
  • Toshihiko Uno
    • 2
  • Yuichi Ohashi
    • 2
    • 4
  1. 1.Department of Ophthalmology and Regenerative MedicineEhime University Graduate School of MedicineToonJapan
  2. 2.Department of OphthalmologyEhime University Graduate School of MedicineToonJapan
  3. 3.Department of Cell Growth and Tumor RegulationEhime University Graduate School of MedicineToonJapan
  4. 4.Department of Infectious DiseasesEhime University Graduate School of MedicineToonJapan

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