Japanese Journal of Ophthalmology

, Volume 52, Issue 2, pp 91–98 | Cite as

Inhibition of choroidal neovascularization by blocking vascular endothelial growth factor receptor tyrosine kinase

  • Junko Kami
  • Kimimasa Muranaka
  • Yasuo Yanagi
  • Ryo Obata
  • Yasuhiro Tamaki
  • Masabumi Shibuya
Laboratory Investigation

Abstract

Purpose

To investigate the role played by receptors of vascular endothelial growth factors, Flt-1 and KDR/Flk-1, on an experimental model of choroidal neovascularization (CNV).

Methods

The vascular endothelial growth factor-A (VEGF-A) receptor-specific tyrosine kinase inhibitor SU5416 was administered to a laser-induced mouse model of CNV. The formation of CNV and the degree of vascular permeability in Flt-1 tyrosine kinase domain-deficient mice were also investigated.

Results

SU5416 reduced vascularity and vascular endothelial cell proliferation, and promoted endothelial cell apoptosis within CNV. Furthermore, the formation of CNV and the degree of vascular permeability were significantly reduced in Flt-1 tyrosine kinase domain-deficient mice, and this effect was enhanced by the administration of SU5416.

Conclusions

Both Flt-1 and KDR/Flk-1 have a significant role in CNV formation. Suppression of apoptosis may be involved in the process.

Key Words

apoptosis choroidal neovascularization SU5416 tyrosine kinase receptor-1 vascular endothelial growth factor-A 

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Copyright information

© Japanese Ophthalmological Society (JOS) 2008

Authors and Affiliations

  • Junko Kami
    • 1
  • Kimimasa Muranaka
    • 1
  • Yasuo Yanagi
    • 1
  • Ryo Obata
    • 1
  • Yasuhiro Tamaki
    • 1
  • Masabumi Shibuya
    • 2
  1. 1.Department of Ophthalmology, Faculty of MedicineUniversity of TokyoTokyoJapan
  2. 2.Department of Genetics, Institute of Medical ScienceUniversity of TokyoTokyoJapan

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