Japanese Journal of Ophthalmology

, Volume 51, Issue 1, pp 10–13 | Cite as

Efficacy of α-Lipoic Acid Against Diabetic Cataract in Rat

  • Masami Kojima
  • Li Sun
  • Ikuho Hata
  • Yasuo Sakamoto
  • Hiroshi Sasaki
  • Kazuyuki Sasaki
LABORATORY INVESTIGATION

Abstract

Purpose

α-Lipoic acid (LA) is well known as a powerful antioxidant. The efficacy of dihydrolipoate-LA for oral administration against streptozotocin (STZ)-induced diabetic cataract in rat was investigated.

Methods

Rats were divided into three groups, control, diabetes mellitus (DM), and DM treated with LA (DM+LA). Diabetes was induced by intravenous injection of 50 mg/kg STZ. DM+LA rats were fed 30 mg/rat per day LA in their diet. Lens changes were assessed using Scheimpflug images (EAS-1000) and by measuring light-scattering intensity.

Results

Increase in lens light scattering was less (P < 0.05) in DM+LA rats than in DM rats 5 weeks after induction of diabetes. DM rats had the highest and control rats the lowest blood glucose levels at every measurement point up to 111 days (P < 0.05).

Conclusion

LA treatment delayed development and progression of cataract in rats with streptozotocin-induced diabetes. Jpn J Ophthalmol 2007;51:10–13 © Japanese Ophthalmological Society 2007

Key words

antioxidant diabetic cataract α-lipoic acid lipoic acid rat 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Packer, L, Witt, EH, Tritschler, HJ 1995α-Lipoic acid as a biological antioxidantFree Radic Biol Med19227250PubMedCrossRefGoogle Scholar
  2. 2.
    Packer, L, Kraemer, K, Rimbach, G 2001Molecular aspects of lipoic acid in the prevention of diabetes complicationsNutrition17888895PubMedCrossRefGoogle Scholar
  3. 3.
    Bron, AJ, Brown, NA, Harding, JJ, Ganea, E 1998The lens and cataract in diabetesInt Ophthalmol Clin383767PubMedCrossRefGoogle Scholar
  4. 4.
    Maitra, I, Serbinova, E, Trischler, H, Packer, L 1995α-Lipoic acid prevents buthionine sulfoximine-induced cataract formation in newborn ratsFree Radic Biol Med18823829PubMedCrossRefGoogle Scholar
  5. 5.
    Maitra, I, Serbinova, E, Tritschler, HJ, Packer, L 1996Stereospecific effects of R-lipoic acid on buthionine sulfoximine-induced cataract formation in newborn ratsBiochem Biophys Res Commun221422429PubMedCrossRefGoogle Scholar
  6. 6.
    Packer, L 1994Antioxidant properties of lipoic acid and its therapeutic effects in prevention of diabetes complications and cataractsAnn N Y Acad Sci738257264PubMedCrossRefGoogle Scholar
  7. 7.
    Kilic, F, Handelman, GJ, Traber, K,  et al. 1998Modelling cortical cataractogenesis XX. In vitro effect of α-lipoic acid on glutathione concentrations in lens in model diabetic cataractogenesisBiochem Mol Biol Int46585595PubMedGoogle Scholar
  8. 8.
    Kilic, F, Handelman, GJ, Serbinova, E, Packer, L, Trevithick, JR 1995Modelling cortical cataractogenesis 17: in vitro effect of α-lipoic acid on glucose-induced lens membrane damage, a model of diabetic cataractogenesisBiochem Mol Biol Int37361370PubMedGoogle Scholar
  9. 9.
    Borenshtein, D, Ofri, R, Werman, M,  et al. 2001Cataract development in diabetic sand rats treated with α-lipoic acid and its gamma-linolenic acid conjugateDiabetes/Metab Res Rev174450CrossRefGoogle Scholar
  10. 10.
    Kilic, F, Trevithick, JR 1995Modelling cortical cataractogenesis. 16. Leakage of lactate dehydrogenase: a new method for following cataract development in cultured lensesBiochem Mol Biol Int3511431152PubMedGoogle Scholar
  11. 11.
    Suzuki, YJ, Tsuchiya, M, Packer, L 1992Lipoate prevents glucose-induced protein modificationsFree Radic Res Commun17211217PubMedGoogle Scholar
  12. 12.
    Ou, P, Nourooz-Zadeh, J, Tritschler, HJ, Wolff, S 1996Activation of aldose reductase in rat lens and metal-ion chelation by aldose reductase inhibitors and lipoic acidFree Radic Res25337346PubMedCrossRefGoogle Scholar
  13. 13.
    Kojima, M, Sasaki, K 1992Application of a new Scheimpflug camera (EAS-1000) to animal cataract modelsOphthalmic Res2439PubMedGoogle Scholar
  14. 14.
    Obrosova, I, Cao, X, Greene, DA, Stevens, MJ 1998Diabetes-induced changes in lens antioxidant status, glucose utilization and energy metabolism: effect of dl-α-lipoic acidDiabetologia4114421450PubMedCrossRefGoogle Scholar
  15. 15.
    Swamy-Mruthinti, S, Green, K, Abraham, EC 1996Scheimpflug densitometric analysis of cataracts in diabetic rats: correlation with glycationOphthalmic Res28230236PubMedCrossRefGoogle Scholar
  16. 16.
    Khamaisi, M, Rudich, A, Potashnik, R,  et al. 1999Lipoic acid acutely induces hypoglycemia in fasting nondiabetic and diabetic ratsMetabolism48504510PubMedCrossRefGoogle Scholar
  17. 17.
    Coppey, LJ, Gellett, JS, Davidson, EP, Dunlap, JA, Lund, DD, Yorek, MA 2001Effect of antioxidant treatment of streptozotocin-induced diabetic rats on endoneurial blood flow, motor nerve conduction velocity, and vascular reactivity of epineurial arterioles of the sciatic nerveDiabetes5019271937PubMedGoogle Scholar
  18. 18.
    Cameron, NE, Cotter, MA, Horrobin, DH, Tritschler, HJ 1998Effects of α-lipoic acid on neurovascular function in diabetic rats: interaction with essential fatty acidsDiabetologia41390399PubMedCrossRefGoogle Scholar
  19. 19.
    Hounsom, L, Horrobin, DF, Tritschler, H, Corder, R, Tomlinson, DR 1998A lipoic acid-gamma linolenic acid conjugate is effective against multiple indices of experimental diabetic neuropathyDiabetologia41839843PubMedCrossRefGoogle Scholar
  20. 20.
    Nagamatsu, M, Nickander, KK, Schmelzer, JD,  et al. 1995Lipoic acid improves nerve blood flow, reduces oxidative stress, and improves distal nerve conduction in experimental diabetic neuropathyDiabetes Care1811601167PubMedGoogle Scholar

Copyright information

© Japanese Ophthalmological Society 2007

Authors and Affiliations

  • Masami Kojima
    • 1
    • 2
  • Li Sun
    • 2
  • Ikuho Hata
    • 2
  • Yasuo Sakamoto
    • 1
    • 2
  • Hiroshi Sasaki
    • 1
    • 2
  • Kazuyuki Sasaki
    • 2
  1. 1.Department of OphthalmologyKanazawa Medical UniversityIshikawaJapan
  2. 2.Division of Vision Research for Environmental Health, Medical Research InstituteKanazawa Medical UniversityIshikawaJapan

Personalised recommendations