Pinus sibirica and P. pumila have a vast hybrid zone, and its structure and maintenance model have not yet been considered. This study was carried out in the southern–western part of the hybrid zone. On the Khamar-Daban Ridge, there is a vigorous belt of mountain taiga forest, where the prevalent species is P. sibirica and where P. pumila produces a subalpine tree belt. The border between these belts is blurred, and a mixed zone of the species is formed, resulting in interspecies hybridization. Field observations, seed efficiency determination, and analysis of mt- and cpDNA markers were used to elucidate the pattern of this hybridization. Pinus sibirica and P. pumila hybridization was determined as bidirectional, and P. pumila was mainly the mother plant. Hybridization transforms to an introgression, and based on the life form, the studied hybrids were divided into two groups: intermediate and pumila-like cup-shaped forms. Both morphological types of hybrids had significantly fewer sound seeds per cone than the parental species. However, pumila-like hybrids had more sound seeds per cone compared to intermediate hybrids. We observed a positive correlation between hybrid seed efficiency and elevation. Summarizing the results of this study and those previously obtained enabled us to show different hybridization patterns in different parts of the hybrid zone, suggesting a mosaic model of hybrid zone maintenance.
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Abbott RJ, Brennan AC (2014) Altitudinal gradients, plant hybrid zones and evolutionary novelty. Philos Trans R Soc B 369:20130346. https://doi.org/10.1098/rstb.2013.0346
Abbott R, Albach D, Ansell S et al (2013) Hybridization and speciation. J Evol Biol 26:229–246. https://doi.org/10.1111/j.1420-9101.2012.02599.x
Arno SF, Hoff RJ (1989) Silvics of whitebark pine (Pinus albicaulis). Gen Tech Rep INT-253. UT: U.S. Department of Agriculture Forest Service Intermountain Research Station, Ogden
Arnold ML, Bulger MR, Burke JM, Hempel AL, Williams JH (1999) Natural hybridization: how low can you go and still be important? Ecology 80:371–381. https://doi.org/10.2307/176618
Barton NH, Hewitt GM (1985) Analysis of hybrid zones. Ann Rev Ecol Syst 16:113–148
Bobrov EG (1961) Introgressive hybridization in the flora of Baikal Siberia. Botan J 46(3):313–327 (in Russian)
Brown IR (1971) Flowering and seed production in grafted clones of Scots pine. Silvae Genet 20(4):121–132
De La Torre AR (2015) Genomic admixture and species delimitation in forest trees. In: Pontarotti P (ed) Evolutionary Biology: Biodiversification from Genotype to Phenotype. Springer, Cham. https://doi.org/10.1007/978-3-319-19932-0_15
De La Torre AR, Roberts DR, Aitken SN (2014a) Genome-wide admixture and ecological niche modelling reveal the maintenance of species boundaries despite long history of interspecific gene flow. Mol Ecol 23:2046–2059. https://doi.org/10.1111/mec.12710
De La Torre AR, Wang T, Jaquish B, Aitken SN (2014b) Adaptation and exogenous selection in a Picea glauca × Picea engelmannii hybrid zone: implications for forest management under climate change. New Phytol 201:687–699. https://doi.org/10.1111/nph.12540
Demesure B, Sodzi M, Petit RJ (1995) A set of universals primers for amplification of polymorphic non-coding regions of mitochondrial and chloroplast DNA in plants. Mol Ecol 4:129–131. https://doi.org/10.1111/j.1365-294x.1995.tb00201.x
Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:11–15
Fernando DD, Lazzaro MD, Owens JN (2005) Growth and development of conifer pollen tubes. Sex Plant Reprod 18:149–162. https://doi.org/10.1007/s00497-005-0008-y
Gebauer R, Volařík D, Funda T, Fundová I, Kohutka A, Klapetek V, Martinková M, Anenkhonov OA, Razuvaev A (2010) Pinus pumila growth at different altitudes in the Svyatoi Nos Peninsula (Russia). J For Sci 56(3):101–111
Goroshkevich SN (1999) On the possibility of natural hybridization between Pinus sibirica and Pinus pumila (Pinaceae) in the Baikal region. Bot J 84(9):48–57 (in Russian)
Goroshkevich SN (2004) Natural hybridization between Russian stone pine (Pinus sibirica) and Japanese stone pine (Pinus pumila). In: Sniezko RA et al. (ed) Breeding and genetic resources of five-needle pines: growth, adaptability, and pest resistance; 2001 July 23–27; Medford, OR, USA. IUFRO working party 2.02.15. U.S. Department of Agriculture, Forest Service, Rocky Mountain Research Station, Proceedings RMRS-P-32. Fort Collins, CO, pp 169–171
Goroshkevich SN, Khutornoy OV (1996) The intrapopulation diversity of cones and seeds of Pinus sibirica Du Tour. Note 1. Level and pattern of variability in the traits. Rastit Resur 32(3):1–12 (in Russian)
Goroshkevich SN, Kustova EA (2002) Morphogenesis of prostrate life form in Siberian stone pine at upper limit of distribution in the Western Sayan Mountains. Russ J Ecol 4:243–249 (in Russian)
Goroshkevich SN, Popov AG, Vasilieva GV (2008) Ecological and morphological studies in the hybrid zone between Pinus sibirica and Pinus pumila. Ann For Res 51:43–52
Gugerli F, Senn J, Anzidei M, Madaghiele A, Büchler U, Sperisen C, Vendramin JJ (2001) Chloroplast microsatellites and mitochondrial nad1 intron 2 sequences indicate congruent phylogenetic relationships among Swiss stone pine (Pinus cembra), Siberian stone pine (Pinus sibirica), and Siberian dwarf pine (Pinus pumila). Mol Ecol 10:1489–1497
Hagman M, Mikkola L (1963) Observations on cross-, self- and interspecific pollinations in Pinus peuce Griseb. Silvae Genet 12:73–79
Harrison RG, Rand DM (1989) Mosaic hybrid zones and the nature of species boundaries. In: Otte D, Endler JA (eds) Speciation and its consequences. Sinauer, Sunderland, pp 111–133
Kagawa K, Takimoto G (2018) Hybridization can promote adaptive radiation by means of transgressive segregation. Ecol Lett 21(2):264–274. https://doi.org/10.1111/ele.12891
Kriebel HB (1972) Embryo development and hybridity barriers in the white pines (Section Strobus). Silvae Genet 21:39–44
Krylov GV, Talantsev NK, Kozakova NF (1983) Siberian stone pine. Lesnaya promyshlenost, Moscow (in Russian)
Mallet J (2009) Rapid speciation, hybridization and adaptive radiation in the Heliconius melpomene group. In: Butlin R, Bridle J, Schluter D (eds) Speciation and patterns of diversity. Cambridge University Press, Cambridge, pp 177–194
Mirov NT (1967) The genus Pinus. Ronald Publications, New York
Mogensen HL (1996) The hows and whys of cytoplasmic inheritance in seed plants. Am J Bot 83:383–404. https://doi.org/10.2307/2446172
Moore WS (1977) An evaluation of narrow hybrid zones in vertebrates. Q Rev Biol 52:263–278. https://doi.org/10.1086/409995
Nechaev VA (2013) Bird biocenotic connections with the mountain pine (Pinus pumila). Bull North East Sci Cent 1:49–59 (in Russian)
Nekrasova TP (1972) Biological aspects of the seed production in Siberian stone pine. Nauka, Novosibirsk (in Russian)
Owens JN (2004) The reproductive biology of western white pine. Forest Genetics Council of B.C. Extension Note 04, April 2004, p 40
Owens JN, Thanong K, Mahalovich MF (2008) Whitebark pine (Pinus albicaulis Engelm) seed production in natural stands. For Ecol Manag 255:803–809. https://doi.org/10.1016/j.foreco.2007.09.067
Petrova EA, Goroshkevich SN, Belokon MM, Belokon YS, Politov DV (2008) Population genetic structure and mating system in the hybrid zone between Pinus sibirica Du Tour and P. pumila (Pall.) Regel at the eastern Baikal Lake shore. Ann For Res 51:19–30
Petrova EA, Zhuk EA, Popov AG, Bondar AA, Belokon MM, Goroshkevich SN, Vasilyeva GV (2018) Asymmetric introgression between Pinus sibirica and Pinus pumila in the Aldan plateau (Eastern Siberia). Silvae Genet 67:66–71. https://doi.org/10.2478/sg-2018-0009
Rauh W (1978) Die Wuchs- und Lebensformen der tropischen Hochgebirgs-Regionen und der Subantarktis, ein Vergleich. In: Troll C, Lauer W (eds) Geoecological relations between the southern temperate zone and the tropical mountains. Steiner, Wiesbaden, pp 62–92
Rieseberg LH, Carney SE (1998) Plant hybridization. New Phytol 140:599–624
Sarvas R (1962) Investigation on the flowering and seed crop of Pinus sylvestris. Commun Inst For Fenn 53(4):1–198
Shcherbakova MA (1965) Detection of quality conifer seed by X-ray analysis. Forest and Timber Institute SB of USSR Academy of Sciences, Krasnoyarsk (in Russian)
Soltis PS, Soltis DE (2009) The role of hybridization in plant speciation. Ann Rev Plant Biol 60:561–588. https://doi.org/10.1146/annurev.arplant.043008.092039
Starikov GF (1961) Siberian dwarf pine in the extreme northeast of its areal. Lesnoye khozaystvo 2:19–20 (in Russian)
Tranquillini W (1979) Physiological ecology of the alpine timberline. Springer, Heidelberg. https://doi.org/10.1007/978-3-642-67107-4
Tretyakova IN (1990) Embryology of conifers: physiological aspects. Nauka, Novosibirsk (in Russian)
Tretyakova IN, Lukina AV (2017) Embryological peculiarities of interspecific hybridization in Pinus sibirica. Russ J Dev Biol 48:340–346. https://doi.org/10.1134/S1062360417050083
Tyulina OV (1976) Moist vegetation belt in Baikal region. Nauka, Novosibirsk (in Russian)
Vasilyeva GV (2014) Seed efficiency of hybrids between Siberian stone pine and Siberian dwarf pine from northern slope of Khamar-Daban ridge. Vestn MGUL Lesn Vestn For Bull 1:85–89 (in Russian)
Vasilyeva GV, Goroshkevich SN (2013) Crossability of Pinus sibirica and P. pumila with their hybrids. Silvae Genet 62:61–68. https://doi.org/10.1515/sg-2013-0008
Vasilyeva GV, Semerikov VL (2014) Application of amplified fragment length polymorphisms markers to study the hybridization between Pinus sibirica and P. pumila. Ann For Res 57:175–180. https://doi.org/10.15287/afr.2014.219
Watano Y, Imazu M, Shimizu T (1996) Spatial distribution of cpDNA and mtDNA haplotypes in a hybrid zone between Pinus pumila and P. parviflora var. pentaphylla (Pinaceae). J Plant Res 109:403–408. https://doi.org/10.1007/BF02344555
Wilhelm R, Hilbish TJ (1998) Assessment of natural selection in a hybrid population of mussels: evaluation of exogenous versus endogenous selection models. Mar Biol 131:505–514. https://doi.org/10.1007/s002270050342
This work was supported by Russian Academy of Sciences (Program of Basic Research in State Academies, Theme 52.2.6) and Russian Foundation for Basic Research (Project No. 18-04-00833).
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Vasilyeva, G., Bondar, A. & Goroshkevich, S. What does a mixed population of Pinus sibirica and P. pumila from the southern Baikal region suggest about the structure of their hybrid zone?. Eur J Forest Res (2020). https://doi.org/10.1007/s10342-019-01254-7
- Subgenus Strobus
- Seed production
- Life form