Advertisement

European Journal of Forest Research

, Volume 138, Issue 6, pp 981–989 | Cite as

Variability of cone parameters and scale morphology in the black alder (Alnus glutinosa L.) in the context of seed extraction

  • Monika AniszewskaEmail author
  • Ewa Tulska
  • Katarzyna Żurawska
Open Access
Original Paper
  • 379 Downloads

Abstract

In this paper, the results of research on the variability of black alder cones (Alnus glutinosa L.) ware presented. The research was carried out for two, significantly different, batches of cones. Basic size parameters and mass were measured. The shape of the cone was determined, and it was described with the fourth-degree polynomial. The surface area and volume of the cone were calculated using the forming curve and formulas for solids: barrel and cylinder. The parameters of cones—shape surface area and volume—were analyzed. It was found that for alder cones (from the researched origins), the average volumes calculated from the barrel formula are 1701 mm3 and 1162 mm3, and the areas calculated from the cylinder formula are 807 mm2 and 597 mm2. The structure of the inner and outer sides of scales was examined using a scanning electron microscope. Using the MultiScanBase v. 18.03 program, the elements of husk structure that could affect the efficiency of seeds extraction were measured. The results of the research can be used to program the process of seeds extraction from alder cones in commercial installations.

Keywords

Cone Scale Surface area Volume Electron microscope 

Introduction

The alder (Alnus) naturally occurs in European forests, but small clusters of these trees may be also found in harsh environmental conditions in northwestern Africa, northern Turkey, the Caucasus, and some valleys of the Atlas Mountains (Claessens et al. 2010). Three alder species are widespread in Europe: two trees (black and grey alder) and one shrub (green alder). According to Murat (2002), Alnus trees may be planted as part of silvicultural treatments and phytomelioration, mostly in pine stands, as well as in the process of reconversion of degraded soils. Due to their constant demand for nutrients and a rapid growth rate, Alnus species are used as pioneer plants for the stabilization of river banks (Meyer et al. 2014) and the reclamation of wetland sites, many of which have dried up in recent years (Beatty et al. 2015). Of the three species mentioned above, the most highly valued one is the black alder as it can fix nitrogen from the atmosphere with the process of symbiosis with Frankia bacteria (Bond et al. 1954). Both aqueous and alcohol extracts from the seeds, bark, and leaves of Alnus glutinosa L. exhibit strong antibacterial activity (Abedini et al. 2016).

Black alder seeds may be collected already from 20-year-old trees, as long as they grow in open land with ample insolation. In dense stands, seeds appear later, at approximately 30 years. Individual alders may produce seeds annually, but more typically every 3 years (Suszka et al. 2000).

Black alder cones are picked manually from standing or lying (felled) trees and then stored in a well-ventilated area. In enclosed spaces, alder cones release seeds within 2–3 weeks. However, due to high requirements and an emphasis on repeatability, it should be noted that such conditions do not afford full control over seed quality. Thus, the process of cone opening should be explored in detail to determine the optimum temperature and humidity parameters with a view to obtaining the highest quality seeds.

The available literature provides information about black alder stands and their growth patterns(Aguinagalde et al. 2005; Lorenc-Plucińska et al. 2013; Vacek et al. 2016; Socha and Ochał 2017), individual trees, wood structure(Yaman 2009; Han et al. 2015), as well as the quality (Kaliniewicz et al. 2018), weight, size, and storage of seeds (Załęski 1995; Aniśko et al. 2006; Bodył 2007; Gosling et al. 2009; Załęski et al. 2009; Kaliniewicz and Trojanowski 2011; Plitta et al. 2013). There is not much information in the available literature regarding seed extraction using kilns or extraction chambers to accelerate scale opening and seed release. According to Murat (2002), black alder cones should be kept in a kiln at 27–38 °C for 6–10 h. Suszka et al. (2000) have reported the extraction process to last 20–25 days at 20 °C and only 1–2 days at 40–45 °C. In practice, the process parameters temperature and humidity and drying time are often set intuitively. After opening, the cones are placed in a rotating drum to obtain seeds. The final moisture content of the seeds after cleaning should be reduced to 3.5% (Aniśko et al. 2006) for storage purposes (Tylkowski 2014). Acquisition of 1 kg of seeds requires the collection and scaling of 15–20 kg of alder cones.

According to the present authors, the current state of knowledge on black alder seed extraction is insufficient, which motivated the study. In order to appropriately design the drying process, the physical parameters of the processed material should be examined, as well as the relationships between them. Thus, the objective of the work was to determine the basic size parameters of black alder cones, including their shape, surface area, volume, as well as the structure of the inner and outer sides of their “pseudo”-scales, which are necessary for developing seed extraction programs under controlled temperature and humidity conditions.

Methodology

Provenance of black alder cones

The study involved closed black alder cones collected in the Maskulińskie Forest District and obtained from a seed extraction facility in Ruciane Nida. The first cone lot was from the Ol40 region with an area of 7.79 ha, registered number MP/3/41009/05. The other lot was of the same provenance, but the cones were collected from a single clone (no. 5534). The cones were harvested in October 2016 and placed in cold storage at 2 °C until measurement.

Size parameters: shape, volume, surface area, and weight

The study material encompassed 100 cones selected at random from two lots (50 from each). Every cone was marked prior to measurements.

In the first step, each cone was weighed with an accuracy of 0.1 mg on a WPA40/160/C/1electronic balance (Radwag, Radom, Poland) designated for precision laboratory weighing. Next, the actual volume of black alder cones (Vact) was measured using a 10-mL NS10/19 graduated cylinder (Alchem, Poland) filled with distilled water, with an accuracy of 0.2 mL.

Cones were photographed with a Nikon D3000 (Nikon, Tokyo, Japan) camera with AF-S DX NIKKOR 18–105 mm f/3.5–5.6G ED VR lens. Marked cones were placed on table next to the manual caliper (Modeco MN 85-001, Poland) which was a measurement standard, used later for scaling. Pictures of cones were made in the resolution of 3888 × 2126 pixels, saved on computer hard disk in JPG and RAW format. They were made on a white background with one camera position. The distance between the lens and the cones was 350 mm. The length of the cone (h) from the top to the base, the maximum thickness (D), and the current diameter with the interval of 0.1 cm (dx) were measured in the cone pictures using the MultiScanBase v. 18.03 program (Computer Scanning System, Warsaw, Poland). The measuring points were determined manually after image scaling and application of grid with mesh size of 1 mm × 1 mm. No additional morphological transformations of the image were used, images were not filtered, and the option “automatically” was chosen.

These measurements were used to calculate the surface area of the examined cones. Each cone was treated as a solid of revolution, and a generating curve for the surface of the solid was defined. The point of origin of the coordinate system was adopted as the distance between the cross section and the base of the cone (Gawart and Mikłaszewicz 2000; Aniszewska 2012).

The cross-section (xj) and radius (rj) coordinates for each cone were used to approximate an equation defining the generating curve for the cone surface. Accuracy was evaluated by minimizing differences between squared function values and squared actual values \(x^{2}\).
$$x^{2} = \mathop \sum \limits_{j = 1}^{n} (y_{ij} - r_{j} )^{2}$$
(1)
where yij—value of ith function at jth cone cross section, rj—radius at jth cross section, n—number of cone cross sections (Gawart and Mikłaszewicz 2000)
Given that the shape function y = f(x) is continuous and nonnegative for the entire cone height (h), the surface area (Scal) was calculated using the following formula:
$$S_{\text{cal}} = 2 \cdot \pi \mathop \int \limits_{a}^{b} y\,{\text{d}}L = 2 \cdot \pi \mathop \int \limits_{0}^{h} y\sqrt {1 + \left( {\frac{{{\text{d}}y}}{{{\text{d}}x}}} \right)^{2} } {\text{d}}x$$
(2)
where dL is the differential of the generating curve.
For the sake of comparison, cone surface area was also calculated by means of another formula treating cones as cylinder-like solids (Sc).
$$S_{\text{c}} = \pi \cdot D \cdot h$$
(3)
Cone volume (Vcal) was calculated using the following formula:
$$V_{\text{cal}} = \pi \mathop \int \limits_{0}^{h} y^{2} \,{\text{d}}x$$
(4)
For comparison, volume was computed using a formula treating cones as barrels (Vb) with elliptical generating curves:
$$V_{\text{b}} = \frac{1}{12} \cdot \pi \cdot \left( {2 \cdot D^{2} + d^{2} } \right)$$
(5)
where D—midplane diameter (largest diameter of the cone), d—diameter of the base.

In this formula, d was the sum of the base (dI) and tip (dII) of the cone divided by two.

Structural studies of black alder scales

Structural studies of the inner and outer surfaces of black alder scales were carried out at the Analytical Center of the Warsaw University of Life Sciences. Selected parts of scales from the middle region of the cones were examined using an FEI Quanta 200 ESEM scanning electron microscope with an EDS EDAX analyzer (ThermoFisher Scientific, USA). The scales were placed on a tray that was inserted in the microscope sample chamber. After closing the chamber, the air was evacuated. Subsequently, each sample was photographed at 50 × , 500 × , and 1000 × magnifications. Images were recorded in.tiff format at 2048 × 1886 pixels and a resolution of 190 dpi.

Statistical analysis

Cone size parameters, weight, surface area, and volume were statistically analyzed using Statistica 13 software (Dell Inc. 2016). Normality of distribution of the variables was evaluated by means of the Shapiro–Wilk test. Selected parameters were compared using ANOVA. All analyses were performed at a significance level of 0.05.

Results

Size parameters: shape, volume, surface area, and weight

The height of cones from the first lot ranged from 13.40 to 26.70 mm (with a mean M = 19.24 mm), while their diameter ranged from 10.30 to 16.00 mm (M = 12.61 mm). In the second lot, cone height was 13.90–21.50 mm (M = 16.53 mm), with a diameter of 9.60–13.10 mm (M = 11.44 mm) (Table 1).
Table 1

Descriptive statistics for the size parameters of cones from two lots

Lot

Data

Mean

SD

Min

Max

Range

Coefficient of variation

1

Height h1 (mm)

19.24

3.87

13.40

26.70

13.30

20.12

 

Diameter D1 (mm)

12.61

1.63

10.30

16.00

5.70

12.93

 

Diameter d1 (mm)

2.21

0.84

0.70

4.50

3.80

38.01

 

Weight m1 (g)

0.298

0.135

0.155

0.749

0.593

45.269

 

Volume, Vcal1 (mm3)

1770.72

849.00

789.79

3945.28

3155.49

47.95

 

Volume Vb1 (mm3)

1701.14

799.27

760.57

3639.70

2879.13

46.98

 

Actual volume Vact1 (mm3)

1600.00

600.00

800.00

2800.00

2000.00

41.44

 

Surface area Scal1 (mm2)

682.73

325.45

358.78

1561.97

1203.19

47.67

 

Surface area Sc1 (mm2)

806.87

302.46

433.60

1733.20

1299.60

37.49

2

Height h2, mm

16.53

1.68

13.90

21.50

7.60

10.18

 

Diameter D2 (mm)

11.44

0.76

9.60

13.10

3.50

6.66

 

Diameter d2 (mm)

1.75

0.36

1.30

2.65

1.35

20.72

 

Weight m2 (g)

0.26

0.05

0.16

0.39

0.22

19.18

 

Volume Vcal2 (mm3)

1228.65

348.97

630.36

1913.06

1282.70

28.40

 

Volume Vb2 (mm3)

1161.58

253.40

701.24

1951.10

1249.86

21.82

 

Actual volume Vact2 (mm3)

1100.00

280.00

800.00

1800.00

1000.00

24.60

 

Surface area Scal2 (mm2)

492.39

119.12

315.68

918.95

603.27

24.19

 

Surface area Sc2 (mm2)

596.85

94.44

434.29

884.83

450.54

15.82

Analysis of relationships between the basic size parameters revealed that height and diameter were significantly correlated for the first lot of cones (0.866): a 1-mm increase in height corresponded to a 0.36-mm increase in diameter. These parameters were also significantly correlated in the second lot (0.701), at a ratio of 1–0.32 mm.

Cones from the first lot were characterized by weight (Table 1), with the extreme values being 0.156 g and 0.749 g (M = 0.298 g). The weight of cones from the second lot ranged from 0.162 to 0.387 g (M = 0.260 g).

The shape of black alder cones was best described by a fourth-degree polynomial as its correlation coefficient (R) was higher than that for second- and third-degree polynomials, and only insignificantly lower than that for a fifth-degree polynomial. Sample shapes of a large cone (no. 2.39) and a small cone (no. 2.14) from the second lot are given in Fig. 1.
Fig. 1

Sample plots of generating curves for the shapes of larger (no. 2.39—blue points) and smaller (no. 2.14—red points) black alder cones from the second lot and their mathematical equation. (Color figure online)

The proposed general equation for the generating curve of cone shape is as follows:
$$y = A \cdot x^{4} + B \cdot x^{3} + C \cdot x^{2} + D \cdot x + E$$
(6)
where x ranges from 0 to h, with y being the cone radius.
Furthermore, the relationship between the examined cone parameters (height and diameter) and the coefficients of the shape equation (A, B, C, D, E) was examined. Despite the high variability of the coefficients, in the first lot, significant correlations were found for all of them, and in particular between A, C, E and cone height, and between B, D and cone diameter. In the second lot, significant correlations were also found between coefficients A, C, E and cone height, while B and D did not reveal any correlations. Correlation equations and correlation coefficients for both lots are presented in Table 2.
Table 2

Correlation equations and correlation coefficients between the coefficients of cone shape generating curves for the first and second lot sand black alder cone height and diameter

Coefficient

Regression equation

Correlation coefficient

Lot 1

Lot 2

Lot 1

Lot 2

A

0.0001 h − 0.0028

0.0001 h − 0.0028

0.8541

0.6300

B

− 0.0054 D + 0.0915

0.6693

C

0.0256 h − 0.8049

0.0200 h − 0.7093

0.8193

0.4543

D

− 0.0949 D  + 3.1556

0.4362

E

0.0638 h + 0.2778

0.0716 h − 0.2006

0.6724

0.3888

Coefficients A, B, C, D, and E for both cone lots were subjected to ANOVA, which showed that the effects were significant depending on p, where p < 0.0500. According to ANOVA, coefficients B (F = 4.5151, p = 0.0376), C (F = 7.0285, p = 0.01017), D (F = 10.3675, p = 0.0020), and E (F = 33.0702, p = 0.0001) for the two lots were significantly different, in contrast to coefficient A, for which p > 0.0500.

The cone surface areas and volumes calculated from Formulas 2 and 4 are given in Table 1. The mean surface area was 6.82 (± SD 3.25) cm2 for the first lot and 4.92 (± SD 1.19) cm2 for the second lot, with the mean volume being 1.77 (SD ± 0.85) cm3 and 1.23 (SD ± 0.35) cm3 for the first and second lots, respectively.

The surface areas calculated from the formula for a cylinder (3) and volumes computed from the formula for a barrel (5) are presented in Table 1. The mean surface area was 8.06 (± SD 3.02) cm2 for the first lot and 5.96 (± SD 0.94) cm2 for the second lot, with the mean volume being 1.70 (SD ± 0.80) cm3 and 1.16 (SD ± 0.25) cm3 for the first and second lots, respectively.

When comparing the values of the actual volume Vact, for both batches, they have a lower value than the volume calculated from the forming curve Vcal by an average of about 0.170 cm3 and 0.130 cm3, and lower than Vb by 0.100 cm3 and 0.061 cm3, respectively (Table 1). A comparison of surface area computed for the two lots using the curve Scal shows that the results were on average lower relative to the values obtained for Sc by 0.124 cm2 and 0.104 cm2. Therefore, to arrive at cone volume and surface area based on height (h) and diameters (D and d), the volume calculated from the formula for a barrel (Vb) should be multiplied by 0.77 and 0.92 for the first and second lots, respectively, while the surface area calculated from the formula for a cylinder (Sc) should be multiplied by 0.81 for both lots.

Analysis of variance of the measured (actual) and calculated cone parameters showed that except for cone weight (F = 2.8180 and p = 0.0982), all size parameters differed significantly, with the results being F = 13.1755 at p = 0.0006 for height, F = 13.5740 at p = 0.0005 for maximum diameter, F = 8.0831 at p = 0.0060 for base diameter, and F = 5.0777 at p = 0.0278 for actual volume. In the case of calculated values, the ANOVA results were as follows F = 11.1600 at p = 0.0014 for volume calculated from the generating curve, F = 13.2508 at p = 0.0006 for volume calculated from the barrel formula, F = 9.6525 at p = 0.0029 for surface area calculated from the generating curve, and F = 14.0589 at p = 0.0004 for surface area calculated from the cylinder formula.

Characterization of black alder scale structure

Each scale consists of an inner and outer side (Aniszewska and Bluszkowska 2016; Aniszewska et al. 2017a). Figure 2 shows SEM images of the inner side of scales, which is inhomogeneous consisting of elements with different characteristics with longitudinal tissues and some projections. Figure 2a presents an entire scale under 50 × magnification, and Fig. 2c–d, e shows fragments under 500 × and 1000 × magnification. The image in Fig. 2c shows the lower part of a scale with a ribbed structure, with parallel bands of cells adjoining one another. The surface of the bottom part of the scale revealed an irregular pattern of narrow chains of cells.
Fig. 2

Inner side of black alder scale: a entire scale under × 50 magnification, b bottom part of the scale (× 1000), c, d middle part of the scale (× 500), e upper part of the scale (× 1000), I—bottom (at the axis), II—middle, III—top

These narrow cells are not found in fragments from the middle part of the scale, which bears the seeds. In that area, the structure of the scale appears smoother than in the bottom part (near the axis). Between rows of cells, there are round, thin-walled cells forming bundle-like structures (see outlined areas in Fig. 2c), which could be seed attachment points.

Figure 2e shows a fragment of the upper part of the scale, which differs significantly from the middle and bottom ones. It reveals a rough surface made of thick-walled cells with visible lumina. That part of the scale has a layered structure, which resembles that of an apophysis, as in pine cones (Gawart and Mikłaszewicz 2000; Aniszewska 2001; Aniszewska and Bluszkowska 2016).

Figure 3 presents photomicrographs of the outer part of the scale under 50 × magnification: its upper part (3a) and the middle and bottom parts (3b). Close-ups of selected areas were taken (as marked in the figure). As can be seen, the scale consists of several thickened layers, which are fused near the cone axis. Figure 3c presents the bottom part of the scale, which is structurally similar to the inner side of the base of the scale. There are also regularly arranged bands of thick-walled cells forming a uniform tissue.
Fig. 3

Outer side of black alder scale: a, b entire scale under × 50 magnification, c bottom part of the scale (× 500), d middle part of the scale (× 1000), e, f upper part of the scale (× 500), I—bottom (at the axis), II—middle, III—top

The middle part also reveals regular bands of cell, with additional transversely oriented thin chains of cells (Fig. 3d). The upper part of the scale (“pseudo-apophysis”) is rough and cauliflower-like; it reveals particles of what may be a resinous substance (Fig. 3e). The pseudo-apophysis is more developed and occupies a larger area on the outer side of the scale as compared to its inner side. In alder cones collected from a single clone, the pseudo-apophasis is clearly divided into two parts at the tip of the scale (Fig. 3a), which gives an impression that it is fused in the middle part of the scale.

Discussion

The studied black alder cones generally fell within the height range reported by other researchers, that is, 1.5–2.5 cm (Suszka et al. 2000). The surface area and volume of individual closed alder cones may be accurately calculated from their height and diameter using a fourth-degree polynomial. However, the proposed model cannot be generalized to other cones as the averaged equation coefficients (A, B, C, D, E) led to significantly overestimated values. The other proposed methods, that is, surface area calculation from a cylinder model and volume calculation from a barrel model, are more useful. They can be readily applied to cones from other lots by defining multipliers. (In the case of the studied cone lots, they amounted to 0.77 and 0.92 for volume and 0.81 for surface area.) The results obtained from these formulas did not differ significantly from actual cone size measurements.

Similar studies have been performed for coniferous species, including the silver fir, Scots pine, and Norway spruce. In those cases, surface area and volume were calculated using the formula for a geometric cone, in addition to a cylinder and a barrel (Gawart and Mikłaszewicz 2000; Aniszewska 2001; Aniszewska and Bluszkowska 2016).

The surface area and volume determined based on the measured size parameters may be helpful in describing changes in moisture content, drying rate, as well as heat and mass transfer, which may be used in designing and programming heat-assisted seed extraction processes in kilns, under commercial conditions, to obtain high-quality seeds for planting. In Poland, the germination capacity of black alder seeds ranges from 35 to 66% (Suszka et al. 2000), and in Ireland from 51 to 56% (De Atrip and O’Reilly 2007).

The described scale structure revealed certain characteristic elements on the inner (concave) side of the scale: bundles of cells which might affect seed release during extraction. The methodology used in this work was previously applied to examine the scales of Scots pine (Aniszewska 2012), European larch (Aniszewska et al. 2017b), and silver fir (Aniszewska et al. 2017a), as well as larch seeds and wings (Aniszewska 2014). It was noted that the outer side of scales differs significantly from the inner side, on which the seeds are borne. The outer side is typically made of evenly sized thick-walled cells, throughout its length. The upper part of pine and fir scales features an apophysis, which is absent in the larch and spruce. On the inner side of the scale, two distinct parts may be observed: one corresponding to the original location of the wings, and the other one outside that area. The former consists of frayed cells, probably formed in the process of detaching the wing and seed from this part of the scale. In other species, this part of scales exhibits cells arranged in chains (Scots pine), small processes or thick-walled cells (in silver fir and Norway spruce). Finally, in the black alder, the inner part of the scale outside the wing area is characterized by regularly arranged cells, similarly to the outer side of the scale.

The presented work describing black alder cones may be treated as a pilot study. The results should be corroborated by research involving a larger number of alder cones of different provenances and collected in different years.

Conclusions

  1. 1.

    Cones from the first batch were larger and had a larger mass compared to cones from the second party. The length was 14% larger, thickness 9% larger, and mass 13% greater.

     
  2. 2.

    The shape of black alder cones is sufficiently well described by a fourth-degree polynomial curve. However, due to substantial differences between the averaged values of the polynomial and the actual cone measurements, this function cannot be used for the calculation of the volume and surface area of other cones, despite the existence of a significant correlation between cone height and diameter.

     
  3. 3.

    The volume and surface area of black alder cones may be calculated using formulas for a barrel and a cylinder, respectively. The values calculated from the former should be multiplied by 0.77 and 0.92 for the first and second lots, respectively, while the values obtained from the latter should be multiplied by 0.81 for both lots. Some small deviations in these coefficients should be expected for other lots of alder cones.

     
  4. 4.

    Examination of the outer and inner sides of black alder scales from two cone lots led to the identification of their characteristic morphological elements with potential implications for cone opening and seed extraction; these include bundles of thin-walled cells on the inner side of the scale (where the seeds are found) and thick-walled cells forming parallel bands of tissues. Resin-like droplets were found on the scales, and especially on the pseudo-apophysis, similarly as in conifers.

     

Notes

References

  1. Abedini A, Chollet S, Angelis A et al (2016) Bioactivity-guided identification of antimicrobial metabolites in Alnus glutinosa bark and optimization of oregonin purification by centrifugal partition chromatography. J Chromatogr, B: Anal Technol Biomed Life Sci 1029–1030:121–127.  https://doi.org/10.1016/j.jchromb.2016.07.021 CrossRefGoogle Scholar
  2. Aguinagalde I, Hampe A, Mohanty A et al (2005) Effects of life history traits and species distribution on genetic structure at maternally inherited markers in European trees and shrubs. J Biogeogr 32(2):329–339.  https://doi.org/10.1111/j.1365-2699.2004.01178.x CrossRefGoogle Scholar
  3. Aniśko E, Witowska O, Załęski A (2006) Wpływ warunków suszenia nasion brzozy brodawkowatej, olszy czarnej, sosny zwyczajnej i świerka pospolitego na ich żywotność. (Effect of drying conditions on viability of common birch, black alder, Scots pine and Norway spruce seeds). For Res Pap 2:91–113Google Scholar
  4. Aniszewska M (2001) Zmienność podstawowych parametrów szyszki świerka. (Variability of major parameters spruce cones). Sylwan 145(6):87–93Google Scholar
  5. Aniszewska M (2012) Dynamika procesu pozyskania nasion w jedno-i dwuetapowych procesach łuszczenia szyszek sosny zwyczajnej (Pinus sylvestris L.). (Dynamics of the process of seed extraction from pine cones Pinus sylvestris L. performed in one or two-steps). Wydawnictwo SGGW. Warszawa ISBN 978-83-7583-393-5Google Scholar
  6. Aniszewska M (2014) Analiza budowy skrzydełek modrzewia europejskiego (Larix decidua Mill.) w aspekcie procesu odskrzydlania. (The analysis of the conformacion of the wings of Larix decidua Mill in terms of dewinging). Stud Mater Cent Eduk Przyr Leśnej Rogów 39(2B):13–19Google Scholar
  7. Aniszewska M, Błuszkowska U (2016) Variability of silver fir (Abies alba Mill.) cones—variability of cone parameters. For Res Pap 77(3):221–229.  https://doi.org/10.1515/frp-2016-0024 CrossRefGoogle Scholar
  8. Aniszewska M, Gendek A, Śliwińska J (2017a) Zmienność szyszek jodły pospolitej (Abies alba Mill.)—zróżnicowanie budowy powierzchni łusek nasiennych. (Variability of silver fir (Abies alba Mill.) cones—variability of scale surface area). For Res Pap 78(1):5–13Google Scholar
  9. Aniszewska M, Stadnik S, Gendek A (2017b) Zmienność budowy szyszek i powierzchni łusek nasiennych modrzewia europejskiego (Larix decidua Mill.). (Variability of cones and scale surface area of European larch (Larix decidua Mill.). For Res Pap 78(3):198–209.  https://doi.org/10.1515/frp-2017-0022 CrossRefGoogle Scholar
  10. Beatty GE, Montgomery WI, Tosh DG, Provan J (2015) Genetic provenance and best practice woodland management: a case study in native alder (Alnus glutinosa). Tree Genet Genomes 11(5):92.  https://doi.org/10.1007/s11295-015-0919-1 CrossRefGoogle Scholar
  11. Bodył M (2007) Mass and viability of European alder (Alnus glutinosa Gaertn.) seeds in the area of Poland during years 1995–2004. Sylwan 151(5):17–22.  https://doi.org/10.26202/sylwan.2006028 CrossRefGoogle Scholar
  12. Bond G, Fletcher W, Ferguson TP (1954) Rozwój i funkcja guzków korzeniowych Albusu. Myricy i Hippophaë. Plant Soil 5(4):309–323CrossRefGoogle Scholar
  13. Claessens H, Oosterbaan A, Savill P, Rondeux J (2010) A review of the characteristics of black alder (Alnus glutinosa (L.) Gaertn.) and their implications for silvicultural practices. Forestry (Lond) 83(2):163–175CrossRefGoogle Scholar
  14. De Atrip N, O’Reilly C (2007) Effect of seed coverings and seed pretreatments on the germination response of Alnus glutinosa and Betula pubescens seeds. Eur J For Res 126(2):271–278.  https://doi.org/10.1007/s10342-006-0146-2 CrossRefGoogle Scholar
  15. Dell Inc. (2016) Dell statistica (data analysis software system), version 13. software.dell.comGoogle Scholar
  16. Gawart B, Mikłaszewicz M (2000) Modele matematyczne kształtu szyszek sosny i świerka. Prz Tech Rol Leśnej 2:20–22Google Scholar
  17. Gosling PG, McCartan SA, Peace AJ (2009) Seed dormancy and germination characteristics of common alder (Alnus glutinosa L.) indicate some potential to adapt to climate change in Britain. For Int J For Res For 82(5):573–582.  https://doi.org/10.1093/forestry/cpp024 CrossRefGoogle Scholar
  18. Han J-H, Jeong J-H, Lee G-Y, Kim B-R (2015) Studies on wood quality and growth of Alnus glutinosa (L.) Gaertn. in Korea—physical properties. J Korean Wood Sci Technol 43(1):1–8.  https://doi.org/10.5658/wood.2015.43.1.1 CrossRefGoogle Scholar
  19. Kaliniewicz Z, Trojanowski A (2011) Variability analysis and correlation of selected physical properties of black alder seeds. Agric Eng 8(133):167–172Google Scholar
  20. Kaliniewicz Z, Markowski P, Anders A, Kaliniewicz Z, Markowski P, Anders A et al (2018) Correlations between germination capacity and selected properties of black alder (Alnus glutinosa Gaertn.) achenes. Balic For 24(1):68–76Google Scholar
  21. Lorenc-Plucińska G, Walentynowicz M, Niewiadomska A (2013) Capabilities of alders (Alnus incana and A. glutinosa) to grow in metal-contaminated soil. Ecol Eng 58:214–227CrossRefGoogle Scholar
  22. Meyer C, Lüscher P, Schulin R (2014) Enhancing the regeneration of compacted forest soils by planting black alder in skid lane tracks. Eur J For Res 133:453–465.  https://doi.org/10.1007/s10342-013-0776-0 CrossRefGoogle Scholar
  23. Murat E (2002) Szczegółowa hodowla lasu. Oficyna Edytorska Wydawnictwo Świat, WarszawaGoogle Scholar
  24. Plitta BP, Michalak M, Kotlarski S, Chmielarz P (2013) Kriogeniczne przechowywanie nasion. (Cryogenic storage of seeds.). Sylwan 157(10):723–729Google Scholar
  25. Socha J, Ochał W (2017) Dynamic site index model and trends in changes of site productivity for Alnus glutinosa (L.) Gaertn. in southern Poland. Dendrobiology 77:45–57.  https://doi.org/10.12657/denbio.077.004 CrossRefGoogle Scholar
  26. Suszka B, Claudine Muller, Bonnet-Masimbert M (2000) Nasiona leśnych drzew liściastych. Od zbioru do siewu. Wydawnictwo Naukowe PWNGoogle Scholar
  27. Tylkowski T (2014) Wpływ łuszczenia i czasu przechowywania nasion olszy czarnej (Alnus glutinosa (L.) Gaertner) na kiełkowanie, wschody i wzrost siewek. (Effect of seed extraction, seed lot, and storage duration on germination capacity and seedling emergence of Alnus glutinosa (L.) Gaertner). Sylwan 158(11):821–828Google Scholar
  28. Vacek Z, Vacek S, Podrázský V et al (2016) Structural diversity and production of alder stands on former agricultural land at high altitudes. Dendrobiology 75:31–44.  https://doi.org/10.12657/denbio.075.004 CrossRefGoogle Scholar
  29. Yaman B (2009) Wood anatomy of ivy-hosting black alder (Alnus glutinosa Gaertn.). Dendrobiology 62:41–45Google Scholar
  30. Załęski A (1995) Nasiennictwo leśnych drzew i krzewów iglastych. Wydawnictwo Świat Książki, WarszawaGoogle Scholar
  31. Załęski A, Aniśko E, Konratowicz W (2009) Zawartość wody w podsuszanych nasionach drzew leśnych a wilgotność względna suszącego powietrza. (Moisture content in dried forest tree seeds versus relative humidity of drying air.). For Res Pap 70(2):151–160Google Scholar

Copyright information

© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  1. 1.Department of Agricultural and Forestry Machinery, Faculty of Production EngineeringWarsaw University of Life Sciences – SGGWWarsawPoland

Personalised recommendations