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European Journal of Forest Research

, Volume 134, Issue 1, pp 53–60 | Cite as

Desiccation tolerance and cryopreservation of seeds of black poplar (Populus nigra L.), a disappearing tree species in Europe

  • Marcin MichalakEmail author
  • Beata P. Plitta
  • Tadeusz Tylkowski
  • Paweł Chmielarz
  • Jan Suszka
Open Access
Original Paper

Abstract

Black poplar (Populus nigra L.) is a widely distributed species that plays a crucial role in riparian forest ecosystems. Due to a reduction in its natural habitats and hybridization with introduced poplar species clones, its genetic pool is decreasing and efforts are required to preserve this species. Seeds of black poplar are short-lived and quickly lose viability during conventional storage in gene banks. Therefore, in order to preserve ex situ the genetic diversity of this species, the feasibility of seed cryopreservation in liquid nitrogen (at −196 °C, LN) for periods of 24 h and 2 years was investigated. Seeds were harvested from three individual trees (two provenances) and desiccated to different levels of water content (WC) in the range of 0.02–0.35 g g−1 (g H2O/g dry mass, g g−1) prior to immersion in LN. Seed germination was assessed after each treatment. P. nigra seeds tolerated desiccation to WC 0.07 g g−1 but after severe desiccation to WC <0.05 g g−1 exhibited a significant reduction in germination. Results indicated that all black poplar seeds, regardless of origin, year of harvesting and seed quality, could be cryopreserved for 24 h when their WC was in the range of 0.11–0.17 g g−1. Physiology of P. nigra seeds showed in this paper is consistent with attributes of intermediate seed storage behavior. This study provides a foundation for using cryopreservation for the ex situ conservation of P. nigra seeds.

Keywords

Black poplar Cryopreservation Rare species Seed desiccation Seed water content Seed storage 

Introduction

Black poplar (Populus nigra L.) has a wide geographic distribution, ranging from West Europe to Central Asia and North Africa (Vanden Broeck 2003). Populus nigra, similar to the majority of Populus species, is a deciduous tree whose seeds are disseminated by both wind and water. It can also propagate vegetatively from broken branches, cuttings, and root suckers. This species is economically important and is used for soil protection and reforestation of polluted industrial zones (Popivshchy et al. 1997). It is also sometimes planted for domestic use (Tunçtaner 1995). P. nigra plays also a central role as a parent pool in poplar breeding programs and has contributed to the breeding of many successful interspecific hybrids (Frison et al. 1995).

Populus nigra is a major species component of softwood floodplain forest ecosystems and plays a critical role in the initial phase of the development of riparian forests. Although some poplar species have fairly large geographic ranges, they are often restricted and exhibit their best development in riparian areas (Wyckoff and Zasada 2005). It has been estimated that up to 99 % of the individuals of this species have disappeared (Lefèvre et al. 1998; Hughes and Rood 2003), mostly as a result of human activities such as the control of river dynamics, wood cutting, and cattle grazing. In recent years, there has been increasing interest in restoring the riparian habitats of European rivers (Arens et al. 1998; Hughes et al. 2005). The genetic diversity of P. nigra, however, is threatened by the reduction of perturbed areas due to the management of river flows, the latter of which has impacted the regeneration of trees all over Western Europe. Massive introduction of P. x euramericana (or P. x canadensis) clones and P. nigra varieties that readily intercross with native P. nigra trees, has also led to a reduction in the genetic diversity of pure P. nigra (Frison et al. 1995) and now poplar is facing extinction (Storme et al. 2004). In Britain and Ireland, P. nigra is one of the most rare tree species (Cottrell 2004).

Wild populations can be a valuable source of new genetic material for plant breeding. The natural diversity of plants growing in their natural habitats means that at least some individuals may carry genes of commercial importance, such as those which confer resistance to diseases and insects or are useful in stressful environments (Acquaah 2007). Therefore, it is crucial to secure as much biodiversity as is possible. Due to its economic value and threatened species status, native populations of P. nigra were considered a high priority for international collaborative activities on forest genetic resources in Europe (Lefevré et al. 1998). Many attempts have been made to assess the genetic diversity of P. nigra populations (Arens et al. 1998; Rathmacher et al. 2010; Smulders et al. 2008). A project for the in situ conservation of this species was also started as part of the European Forest Genetic Resource Programme (Lefèvre et al. 2001). However, protection of high biodiversity of species is not possible on clone plantation, because it needs huge land area and cost of maintaining, moreover, clonal plantation can be affected by disease or pest.

Seed storage is the most efficient method of protection for many species because it enables the preservation and conservation of a huge amount of genetic diversity (Linington and Pritchard 2001). Additionally, ex situ seed conservation is 100 times cheaper that the in situ preservation of individual trees (Li and Pritchard 2009). Populus seeds, however, have a very short life-span and are sensitive to the combined stress of low moisture and temperature during storage (Hill et al. 2013, Popova et al. 2013). So far, storage of several Populus species and hybrids (P. alba, P. alba × P. glandulosa, P. deltoides, P. tremuluoides) have been studied previously (Pence 1996; Hill et al. 2013; Popova et al. 2013). All reports confirmed that standard temperatures of seed banking at 5 or −20 °C are not enough to preserve viability of Populus seeds for long term, as longevity for diverse species at such temperatures is relatively short (3–12 years), (Popova et al. 2013). The only large-scale long-term option for the ex situ conservation of species that have short-lived seeds, which are sensitive to desiccation, is cryopreservation. Storage at temperature below −160 °C is expected to prolong seed life for hundreds or even thousands of years due to very high reduction of metabolic activities (Walters et al. 2004). The most critical factor affecting cryopreservation of seeds is water content (WC), (Pritchard 2007). When dried to an optimal range of WC, the desiccation of seeds prior to cryopreservation in LN confers a significant increase in survival (Engelmann 2000). Several studies have demonstrated that desiccation sensitivity is the critical factor for the cryopreservation of seeds from forest tree species (Chmielarz 2009a, b; Popova et al. 2012).

Seeds depending on their desiccation tolerance and ability to storage were categorized to three groups: orthodox, intermediate, and recalcitrant (Roberts 1973; Ellis et al. 1990). Orthodox seeds are tolerant to very low desiccation (below 0.05 g g−1 of WC) and storage at subzero temperatures. Recalcitrant seeds do not tolerate drying to relative high moisture content (Roberts 1973) and intermediate seed characterized by the combined sensitivity to low moisture content and temperature (Ellis et al. 1990; Hill et al. 2013). Also hydration window—the range of WC, which allows seeds to tolerate cryogenic temperatures may vary between categories and be very narrow for intermediate seeds (Dussert and Engelmann 2006; Hor et al. 2005) or very wide for orthodox seeds (Chmielarz 2010a, b). Whole recalcitrant seeds did not tolerate cryoexposure and for successful cryopreservation embryonic axes or plumules need to be excised (Chmielarz et al. 2011; Plitta et al. 2014).

Tolerance to desiccation and LN exposure of over a dozen Populus and Salix species was investigated (Hong et al. 1998; Maroder et al. 2000; Popova et al. 2012, 2013; Pence 1996; Hill et al. 2013). Most of them (30 species) were considered as orthodox (Hong et al. 1998; Maroder et al. 2000), and some of them (P. tremuloides and P. deltoides) were characterized as intermediate (Pence 1996; Hill et al. 2013). All these reports showed that seed tolerance to WC manipulation and cryopreservation is very variable among species in Salicaceae family (Popova et al. 2013). Therefore, desiccation and storage behavior should be assessed for each species separately and it is difficult to predict in advance to which category investigated seeds should be classified. In the literature, there is information that P. nigra seeds, due to very rapid loss of viability during storage, should be classified as recalcitrant (Gosling 2007). However, neither desiccation tolerance nor tolerance to immersion in LN has been investigated for black poplar seeds, although Tylkowski (2010) stored P. nigra seeds at LN for one day without any negative impact on germination. Also in the literature, there is lack of data about severe drying to 0.05 g g−1 WC or lower of seeds of any Populus sp.

The present study was carried out to quantify the desiccation tolerance of P. nigra seeds and their response to cryostorage for 24 h. We applied to determine the safe range (SR) of water content at which black poplar seeds could be safely cryostored. Determining the optimal WC for the cryopreservation of P. nigra seeds will enable their long-term storage in gene banks tasked with preserving the genetic diversity of forest tree species.

Materials and methods

Plant material

Seeds were collected from three different 50- to 70-year old trees in May 2010 (Seed lot No. 1) and 2011 (Seed lot No. 2) in Czeszewo (middle-west Poland) near the Warta River (52°8′N and 17°30′E) and from one tree in May of 2002 (Seed lot No. 3) in Toruń (centre of Poland) near the Wisła River (53°02′N and 18°43′E). Trees in Czeszewo and Toruń were growing in populations of 80–100 individuals. The distance between two trees collected in Czeszewo was approximately 60 m, and the distance between Czeszewo and Torun is approximately 150 km. Due to the fact that seeds from Seed lot 1 and Seed lot 2 originated from the same population therefore at least some seeds could be half-siblings due to pollination from similar parental tree. There were no other poplar plantations or poplar species nearby, ensuring that the seeds used in the study were true seeds of P. nigra. Catkins were collected directly from the trees when they ripened and started to open. Catkins were placed in an environmental chamber which was maintained at a constant 15 °C for 72 h to allow the catkins to fully open. Seeds were manually separated from their cotton-like seed hairs using a sieve with 2.5 mm holes and then stored in hermetically sealed polyethylene bags at 3 °C for 7 days until the commencement of the experiments.

Adjusting the WC of seeds

Fresh seeds, after extraction from the catkins, had a WC of 0.11 g H2O/g−1 dry mass (g g−1) and were stored at 3 °C prior to further analysis. Seeds were dried or moistened to various WC levels prior to immersion in LN as previously described (Michalak et al. 2013). Seed were moisturized several times with water to obtain ≥0.11 g g−1 WC, and then left in tightly closed vials for 3 days (Seed lot No. 1 and 2) or 7 days (Seed lot No. 3) of conditioning at 3 °C. Seeds at WC of ≤0.11 g g−1 were obtained by desiccation over activated silica gel for about 4–6 h (0.07–0.08 g g−1 WC), 24 h (0.04–0.05 g g−1 WC) or 48 h (0.02 g g−1 WC). Seed lot No. 1 was adjusted to a range of 0.04–0.37 g g−1 WC, Seed lot No. 2 to a of range 0.02–0.35 g g−1 WC, and Seed lot No. 3 to a range of 0.04–0.35 g g−1 WC. Seed WC (3 replications of 50 seeds each) was determined by drying seeds at 103 ± 2 °C for 17 h each time WC was analyzed.

Assessment of a safe range of seed WC

The range of seed WC that is conducive to safe and successful storage in LN was determined by moistening or desiccating seeds to eight (Seed lot No. 1) or eleven (Seed lot No. 2 and No. 3) different levels of WC in the ranges stated previously. Seeds were placed in vials (Nunc 1.8 ml) and plunged directly into LN and stored for 24 h. After storage in LN, vials containing seeds were thawed at 40 °C in a water bath for 5 min. Seeds desiccated to analogous levels of WC were stored for 24 h at 3 °C as controls. As a safe range, we assume seed WC range were germinability of seeds were not affected by pre-treatment before cryostorage (desiccation or conditioning) or by immersion in LN after pre-treatment.

Germination

Germination tests were conducted using 50 seeds placed on moist filter paper (70 mm in diameter) in a Jacobsen apparatus and covered with a plastic lid. This served as one replicate and there were four replicates for each level of desiccation and form of storage. Temperature was maintained at 23 °C for 22 h and 27 °C for 2 h each day, and light was provided on a 12 h cycle (irradiance of 22 μmol m−2 s−1) with illumination being provided during the period with the highest daily temperature. All seeds with an emerging radical, collet hairs, and fully expanded cotyledons after 14 days were considered as germinated.

Statistical analysis

STATISTICA software was used (StatSoft Polska 1995–2005) for analysis of the data. Analysis of variance (ANOVA) was used to analyze for treatment effects and NIR tests were performed after arc-sin transformation of the data at a significance level of P = 0.05. Separate one-way ANOVAs were used for analyzing the effect of different WC and cryopreservation on seeds. Additionally, Tukey’s tests were performed after arc-sin transformation of the data at a significance level of P = 0.05 and a two-way ANOVA analyses for the effect of cryopreservation on seeds with different WC was conducted (Supplementary data).

Results

Desiccation sensitivity of seeds

Fresh, untreated, control seeds from Seed lot No. 1 with a WC of 0.11 g g−1 exhibited 47 % germination. Desiccation of seeds from WC 0.11–0.05 g g−1 reduced their germination to 16 %, while severe desiccation to 0.04 g g−1 WC resulted in a decline of germination to 5 %, (Fig. 1a). Fresh, control seeds from Seed lot No. 2 with a WC of 0.11 g g−1 exhibited a very high level of germination 94 % (Fig. 1b). Drying of these seeds to a WC of 0.08 or 0.07 g g−1 did not significantly reduce their levels of germination (94 and 92 % of germination, respectively). Further drying of these seeds to a WC of 0.04 g g−1 decreased germination to 82 %, while seeds desiccated to the lowest level of WC 0.02 g g−1 germinated at 67 % (Fig. 1b). Seeds that were collected in Toruń and desiccated to a WC of 0.07 g g−1 exhibited the same level of germination (89 %) as control seeds (0.10 g g−1) but desiccation to a WC of 0.4 g g−1 significantly reduced seeds germination to 78 % (Fig. 1c).
Fig. 1

Percentage of germination of P. nigra seeds collected from Seed lot No. 1 (a), Seed lot No. 2 (b), Seed lot No. 3 (c). Seeds desiccated and hydrated to WC levels ranging from 0.04 to 0.37 g g−1 (a), 0.02–0.35 g g−1 (b), and 0.04–0.35 g g−1 (c). Separate statistical analysis was made for each Seed lot. Values labeled with the same lower-case letter are not significantly different at P < 0.05, NIR test

Optimal WC for seed cryopreservation

The range of WC at which germination of cryopreserved seeds was the highest in Seed lot No. 1 was 0.11–0.23 g g−1 (Fig. 2a). In this safe range, the germination of cryostored seeds was 33–40 %, it was similar to the level of germination of seeds that were not subjected to cryostorage (32–52 %). Cryopreserved seeds desiccated to 0.05 or 0.04 g g−1 WC had germination percentage of 15 and 9 %, which were significantly lower than germination of control seeds (not cryopreserved) (Fig. 2 A). Cryopreserved seeds with a higher level of WC (0.29, 0.33 and 0.37 g g−1) than fresh seeds exhibited lower germinability (12, 26 and 23 %, respectively) than fresh seeds after cryopreservation (Fig. 2a). The hydration of seeds to high WCs 0.33 and 0.37 g g−1 and their conditioning for 3 days had no effect on seed germination, however, at WC 0.29 g g−1 seed germination was reduced to 13 % (Fig. 2a).
Fig. 2

Percentage of germination of P. nigra seeds collected from Seed lot No. 1 (a), Seed lot No. 2 (b), Seed lot No. 3 (c). Seeds desiccated and hydrated to WC levels ranging from 0.04 to 0.37 g∙g−1 (a), 0.02–0.35 g g−1 (b), and 0.04-0.35 g g−1 (c) and subjected to cryopreservation for 24 h. Separate statistical analysis was made for each Seed lot. Values labeled with the same lower-case letter are not significantly different at P < 0.05, NIR test. SR*—safe range

Cryopreservation of seeds from Seed lot No. 2 tolerated freezing in LN without any loss in viability when WC was in the range of 0.07–0.17 g g−1. In this safe range of WC, germination of cryopreserved seeds was 86–97 %. This was similar to the level of germination of non-frozen seeds which was 82–98 % (Fig. 2b). Cryopreservation of seeds desiccated to a WC of 0.04 or 0.02 g g−1 exhibited a germination rate of 74 and 64 %, respectively. This level of germination was significantly lower than either cryopreserved or non-frozen control seeds with a WC of 0.11 g g−1. Cryopreservation of seeds with a WC in the range of 0.19–0.35 g g−1 caused a significant reduction in germination from 90 to 38–57 %. Seeds at a high WC (0.19–0.35 g g−1) had a low percentage (22–58 %) of germination, regardless whether or not they were frozen in LN (Fig. 2b).

Seeds from the Toruń Seed lot No. 3 with a wide of WC (0.07–0.17 g g−1) readily survived cryopreservation (Fig. 2c). Highly hydrated seeds (0.31 and 0.35 g g−1) exhibited a significantly lower rate (71 and 56 %, respectively) of germination than control seeds which were approximately 90 %. Seeds moistened to 0.20 and 0.27 g g−1 WC, that were not cryopreserved but only conditioned, had a germination percent of only 55 or 53 %, which was significantly lower than the 90 % of germination observed with control seeds having a water content of 0.10-0.12 g g−1. Severe desiccation of the seeds from Toruń to 0.04 g g−1 WC caused a significant loss in the germinability (Fig. 1c). Based on these results, the safe range of WC for seeds from Toruń was considered as 0.07–0.17 g g−1. Germination of cryopreserved and non-cryopreserved seeds in this safe range was 89–95 % (Fig. 2c).

Discussion

Desiccation tolerance

Seeds of P. nigra from Seed lot No. 2, and Seed lot No. 3 had the highest initial levels of germination (>90 %). These seeds tolerated desiccation to 0.07 g g−1 WC, but significantly lost viability after desiccation to a WC of 0.04 g g−1 or lower. Seeds from Seed lot No. 1 (Fig. 1a), with a WC of 0.11 g g−1, had a much lower initial level of germination (47 %). This level was reduced even further when seeds were desiccated to a WC of 0.05 g g−1. Pence (1996) showed that Populus deltoides seeds survived drying to a WC of 0.08–0.11 g g−1. Similar to our results, short-lived seeds of Salix caprea desiccated from 0.08 to 0.05 g g−1 WC significantly decreased germination from 100 to 19 % (Popova et al. 2012). In S. caprea, desiccation tolerance of seeds was correlated with the initial viability of seeds (Popova et al. 2012). In our opinion, seeds of P. nigra should be classified as intermediate seeds rather than recalcitrant based on their response to desiccation. This classification contradicts a suggestion that was previously provided by Gosling (2007) that P. nigra seeds are recalcitrant, short-lived seeds that can only be stored for four weeks in nondrying conditions at 4 °C without losing viability (Gosling 2007). Recalcitrant seeds are killed by drying them to a WC as high as 0.25–0.43 g g−1 (Pritchard 2004), while intermediate seeds tolerate desiccation to a WC of approximately 0.11–0.13 g g−1 (Hong et al. 1998). Additionally, Populus nigra seeds should not be classified as orthodox because they did not tolerate desiccation to a WC ≤0.05 g g−1 without loss of viability (Roberts 1973). Intermediate seeds lose their viability in cold storage even when they are desiccated (Ellis et al. 1990). Therefore, seeds of P. nigra were subjected to cryostorage in order to prove that they fully fit the classification as intermediate seeds. Our data are in concordance with previous results (Pence 1996; Hill et al. 2013) showing that short-lived seeds of P. deltoides or P. tremuloides should be categorized as intermediate. On the other hand, they are in contradiction with results obtained by Maroder et al. (2000) and Hong et al. (1998) who classified Salix seeds as orthodox. Contrary to our results also P. alba x glandulosa seemed to have orthodox type of behavior (Popova et al. 2013). Our data support the claim that in Salicaceae family desiccation tolerance varies significantly between species (Popova et al. 2013). Therefore, for each species of Salicaceae desiccation, tolerance should be investigated separately.

Increasing the WC of seeds

Increasing the WC of seeds above the level of freshly collected seeds had different effects on germination depending on initial seed viability. Seeds from Seed lot No. 3 (Fig. 2c) and Seed lot No. 2 (Fig. 2b) exhibited a high initial viability (>90 %). Hydration of these seeds to a WC 0.12–0.17 g g−1 did not have any effect on seed germination. However, further hydration to a WC over 0.17 g g−1, resulted in a significant reduction in germination. Similarly negative effect of highly vigorous seed moisturizing was observed during the development of seedlings of S. caprea clones (Popova et al. 2012). Observed decrease in viability of P. nigra seeds after their moisturizing may be connected with aging process which runs faster in seeds at higher WC. Results show that if WC of P. nigra seeds is higher than 0.17 g g−1 they deteriorate very fast even during storage at 3 °C.

In contrast, the germination of seeds from Seed lot No. 1, which had a lower initial viability compared with Seed lot No. 2, was not affected by increasing their level of hydration, with the exception of a WC of 0.29 g g−1. These results were contrasted those obtained by Popova et al. (2012) and Maroder et al. (2000) who reported that lower germination of S. caprea, S. alba and S. matsudana seeds could be improved by moderate rehydration before sowing. The difference in response to rehydration of S. caprea, S. alba and S. matsudana and P. nigra seeds could result from different methodology of rehydration (24 h in case of Salix species and 3–7 day in case of P. nigra seeds) or this trait may be connected with family and different tree species even closely related like Salix and Populus could react at different way to moisturizing of seeds.

Cryopreservation of seeds

Seeds from all of the investigated provenances tolerated cryopreservation when seeds were desiccated prior to immersion in LN to a WC in the ranges defined in our study. Only a slight variation in the safe range of WC was observed for the individual Seed lot. Safe ranges were defined as a WC of 0.11–0.23 g g−1 for Seed lot No. 1 (Fig. 2a) and 0.07–0.17 g g−1 for Seed lot No. 2 (Fig. 2b), and 0.07–0.17 g g−1 for the Seed lot No. 3 (Fig. 2c). WCs in the range of 0.07–0.10 g g−1 were not investigated for Seed lot No. 1 due to an insufficient number of seeds. Our data indicate that in the range of 0.11–0.17 g g−1 WC, seeds tolerated cryopreservation regardless of their initial viability, reaction to water content adjustment, year of collection, or origin. Pence (1996) reported on cryopreservation of seeds of Populus deltoides. Her data indicate that seeds of this species can be successfully cryopreserved when they are desiccated to a WC in the range of 0.085–0.17 g g−1 prior to immersion in LN. Our data indicated that the efficiency of cryopreservation is dramatically reduced when seeds are desiccated to a WC lower than 0.07 g g−1. Thus, the results presented by us and Pence (1996) are in accordance. The safe range (0.11–0.17 g g−1) of WC for the successful cryopreservation of seeds obtained in the present study is narrow in comparison to the safe ranges observed for most of orthodox seeds of other tree species (Table 1) . Only Corylus avellana seeds which were considered as orthodox (Michalak et al. 2013) have more narrow safe range of WC.
Table 1

Safe ranges of seed water content (WC) and moisture content (MC) of forest tree species required for successful cryopreservation

Type of seeds

Species

Safe range WC (g g−1)

Safe range MC (%)

Literature

Orthodox

Black alder (Alnus glutinosa) (L.) Gaertn.

0.026–0.23

2.7–19.2

Chmielarz 2010a

European ash (Fraxinus excelsior) L.

0.075–0.24

7.2–19.5

Chmielarz 2009a

Silver birch (Betula pendula) Roth

0.02–0.31

2.0–23.2

Chmielarz 2010b

European hazelnut (Corylus avellana) L.

0.08–0.10

7.2–9.1

Michalak et al. 2013

Intermediate

Black polar (Populus nigra) L.

0.11–0.17

10–15

present study

Easter cottonwood (Populus deltoides) Bartr.

0.085–0.17

8–15

Pence 1996

Mazzard cherr (Prunus avium) L

0.1–0.2*

9.0–16.9*

Chmielarz 2009b

* After germination, seedling emergence from seeds stored in LN was lower than from non-frozen seed. Seed moisture content (MC) expressed in % (FW fresh weight basis)

Similar to P. nigra seeds narrow safe ranges were observed in investigations concerning cryopreservation of Prunus avium, and Populus deltoides seeds. All species are considered to produce seeds with intermediate type of behavior (Chmielarz 2009b; Pence 1996). This support our findings that even in terms of response to temperature of LN P. nigra react as seeds classified as intermediate.

Previous reports about viability of seeds after storage at subzero temperatures of P. nigra seeds showed that these short-lived seeds could not be long-term stored (Gosling 2007). Therefore, seed banking of P. nigra seeds has not been considered a feasible option. However, results of this study demonstrated that cryostorage of P. nigra seeds is possible, even though the routine use of cryopreservation is still limited. However, growing number of gene banks and botanical gardens use cryopreservation on a large scale for different types of material (Engelmann 2011); for example, the U.S. Department of Agriculture-Agricultural Research Service (USDA-ARS) National Center for Genetic Resources Preservation (NCGRP), Fort Collins, CO, USA (Walters et al. 2004), The National Bureau for Plant Genetic Resources (NBPGR; New Delhi, India), (Mandal 2000) and Center for Conservation and Research of Endangered Wildlife, CryoBioBank, Cincinnati, USA (http://cincinnatizoo.org/conservation/crew/). Cryopreservation is also applied to intermediate seeds, such as coffee seeds which are preserved in the Tropical Agricultural Research and Higher Education Center (CATIE, Cañas, Guanacaste, Costa Rica) and in the IRD (Montpellier, France) (Engelmann 2011). We proofed that cryostroage of P. nigra seeds can be used for long-term protection of the genetic biodiversity of this species in gene banks.

Conclusions

Summarizing P. nigra seeds can be desiccated to a WC of 0.07 g g−1 without a loss in viability. When seeds of P. nigra are exposed to lower levels of WC (≤0.05 g g−1), their viability is significantly decreased. Our data indicate that seeds of this species could be successfully cryopreserved to preserve its genetic diversity if seeds are desiccated to a WC in the safe range of 0.11–0.17 g g−1 prior to immersion in LN without any reduction in the germinability, regardless of their initial viability, origin and year of collection. Based on their desiccation tolerance, ability to withstand immersion in LN, we classified P. nigra seeds as intermediate.

Notes

Acknowledgments

We thank Elżbieta Drzewiecka-Pieniężna, Elżbieta Nogajewska, Danuta Szymańska and Magdalena Sobczak for providing technical assistance. This work was supported by a grant from the National Science Centre, Poland (No. NN309 429 138). We would like to thank Reviewers for their comments which allow us to improve the manuscript.

Supplementary material

10342_2014_832_MOESM1_ESM.doc (88 kb)
Supplementary material 1 (DOC 88 kb)

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© The Author(s) 2014

Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.

Authors and Affiliations

  • Marcin Michalak
    • 1
    Email author
  • Beata P. Plitta
    • 1
  • Tadeusz Tylkowski
    • 1
  • Paweł Chmielarz
    • 1
  • Jan Suszka
    • 1
  1. 1.Institute of DendrologyPolish Academy of SciencesKórnikPoland

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